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• W1520904485 abstract "The specificity of JHM virus (JHMV) tropism for rat oligodendrocytes, as one of the primary host cells in the central nervous system, is maintained after explanation (S. Beushausen and S. Dales, Virology 141:89-101, 1985). The temporal correlation between onset of resistance to JHMV infection in vivo, completion of myelination, and maturation of the central nervous system can be simulated in vitro by inducers of oligodendrocyte differentiation (Beushausen and Dales, Virology, 1985). Stimulation of differentiation through the elevation of intracellular cyclic AMP (cAMP) levels suggests a possible connection between activation of the adenylate cyclase system and coronavirus expression. Chromatographic analysis of cAMP-dependent protein kinase activity in cytosol extracts prepared from astrocytes or oligodendrocytes revealed that both glial cell types were deficient in protein kinase I, indicating that expression of coronavirus in differentiated cells was not contingent upon the presence of protein kinase I. However, treatment with N6,2'-O-dibutyryladenosine-3',5'-cyclic monophosphate (dbcAMP) resulted in a severalfold enhancement of the free regulatory subunit (RI) in oligodendrocytes but not in astrocytes. The RII subunit in both neural cell types was relatively unaffected. Rapid increase in RI due to dbcAMP treatment was correlated with inhibition of JHMV expression. Other differentiation inducers, including 8-Br cAMP and forskolin which, by contrast, caused a decrease in detectable RI, also blocked JHMV expression. This apparent anomaly can be attributed to an increased turnover of RI due to destabilization of the molecule which occurs upon site-specific binding of the cyclic nucleotides. On the basis of these observations, we conclude that the state of oligodendrocyte differentiation manifested with the modulation of RI regulates JHMV expression. The differentiation process did not affect either virus adsorption or sequestration but appeared to inhibit the expression of viral RNA and proteins, implying that replication was inhibited at some step between penetration and initiation of genomic functions, perhaps at the stage of uncoating. We therefore examined the possibility that protein kinases and phosphatases, which influence cellular regulation during cAMP-induced differentiation, may be responsible for the phenomenon of coronavirus suppression in oligodendrocytes. Evidence was obtained indicating that normal processing of the phosphorylated nucleocapsid protein is inhibited in differentiated oligodendrocytes, consistent with the notion that JHMV replication might be arrested during uncoating." @default.
• W1520904485 created "2016-06-24" @default.
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• W1520904485 date "1987-12-01" @default.
• W1520904485 modified "2023-09-27" @default.
• W1520904485 title "In vivo and in vitro models of demyelinating disease: activation of the adenylate cyclase system influences JHM virus expression in explanted rat oligodendrocytes" @default.
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• W1520904485 cites W1512424776 @default.
• W1520904485 cites W1533548516 @default.
• W1520904485 cites W1540912300 @default.
• W1520904485 cites W1542995590 @default.
• W1520904485 cites W1557962201 @default.
• W1520904485 cites W1573309488 @default.
• W1520904485 cites W1580216379 @default.
• W1520904485 cites W1606423922 @default.
• W1520904485 cites W1684900679 @default.
• W1520904485 cites W1775749144 @default.
• W1520904485 cites W1868144737 @default.
• W1520904485 cites W1871643653 @default.
• W1520904485 cites W1964585388 @default.
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• W1520904485 cites W1972749317 @default.
• W1520904485 cites W1972961195 @default.
• W1520904485 cites W1978127297 @default.
• W1520904485 cites W1988832534 @default.
• W1520904485 cites W1993238289 @default.
• W1520904485 cites W1995922530 @default.
• W1520904485 cites W1996144696 @default.
• W1520904485 cites W1996626295 @default.
• W1520904485 cites W2001358864 @default.
• W1520904485 cites W2010820226 @default.
• W1520904485 cites W2018275786 @default.
• W1520904485 cites W2018289835 @default.
• W1520904485 cites W2022377170 @default.
• W1520904485 cites W2024158105 @default.
• W1520904485 cites W2029010432 @default.
• W1520904485 cites W2030903558 @default.
• W1520904485 cites W2035059006 @default.
• W1520904485 cites W2036750551 @default.
• W1520904485 cites W2037582931 @default.
• W1520904485 cites W2038152355 @default.
• W1520904485 cites W2043543206 @default.
• W1520904485 cites W2050621883 @default.
• W1520904485 cites W2051881197 @default.
• W1520904485 cites W2054057759 @default.
• W1520904485 cites W2058545848 @default.
• W1520904485 cites W2059994807 @default.
• W1520904485 cites W2069225289 @default.
• W1520904485 cites W2069919073 @default.
• W1520904485 cites W2082552542 @default.
• W1520904485 cites W2083323266 @default.
• W1520904485 cites W2087355971 @default.
• W1520904485 cites W2087476745 @default.
• W1520904485 cites W2088191598 @default.
• W1520904485 cites W2090501753 @default.
• W1520904485 cites W2091255490 @default.
• W1520904485 cites W2092685741 @default.
• W1520904485 cites W2096504789 @default.
• W1520904485 cites W2098919352 @default.
• W1520904485 cites W2100837269 @default.
• W1520904485 cites W2111290630 @default.
• W1520904485 cites W2122781121 @default.
• W1520904485 cites W2126907710 @default.
• W1520904485 cites W2130286046 @default.
• W1520904485 cites W2131022909 @default.
• W1520904485 cites W2131908157 @default.
• W1520904485 cites W2141202074 @default.
• W1520904485 cites W2142286320 @default.
• W1520904485 cites W2163078273 @default.
• W1520904485 cites W2176157290 @default.
• W1520904485 cites W2206884908 @default.
• W1520904485 cites W2251920431 @default.
• W1520904485 cites W2418791397 @default.
• W1520904485 cites W41162502 @default.
• W1520904485 cites W53423959 @default.
• W1520904485 cites W79754167 @default.
• W1520904485 cites W82941884 @default.
• W1520904485 cites W931622 @default.
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• W1520904485 doi "https://doi.org/10.1128/jvi.61.12.3795-3803.1987" @default.
• W1520904485 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/255995" @default.
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