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• W1520921883 abstract "To see what effect the lesion had on the endocytic pathway, we measured the accumulation and recycling of the fluid-phase marker horseradish peroxidase. Accumulation was inhibited by 50% while recycling was barely affected, suggesting that the rate of fluid-phase endocytosis was reduced. We previously showed that the clathrin-coated pit pathway of endocytosis was not affected in the mutant, indicated by a normal transferrin cycle (Colbaugh, P. A., Stookey, M., and Draper, R. K. (1989) J. Cell Biol. 108,2211-2219). Thus, the secretory lesion correlates with reduced fluid-phase endocytosis without impairing the clathrin-dependent pathway of receptor-mediated endocytosis. We also investigated the delivery of endocytosed material to lysosomes and found that delivery was partially, but not completely, impaired in the mutant. This suggests that endocytosed material can enter lysosomes, although slowly, in the absence of a functional secretory pathway." @default.
• W1520921883 created "2016-06-24" @default.
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• W1520921883 date "1990-11-01" @default.
• W1520921883 modified "2023-09-26" @default.
• W1520921883 title "Impaired secretion and fluid-phase endocytosis in the End4 mutant of Chinese hamster ovary cells" @default.
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• W1520921883 doi "https://doi.org/10.1016/s0021-9258(17)30487-8" @default.
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