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- W1521054785 abstract "The S-nitrosation of protein thiols is a redox-based posttranslational modification that modulates protein function and cell phenotype. Although the detection of S-nitrosated proteins is problematical because of the lability of S-nitrosothiols, an increasing range of proteins has been shown to undergo S-nitrosation with the improvement of molecular tools. This chapter describes the methodology used to identify potential targets of S-nitrosation in cultured primary human hepatocytes using proteomic approaches. This methodology is based on the biotin switch method, which labels S-nitrosated proteins with an affinity tag, allowing their selective detection and proteomic identification." @default.
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- W1521054785 date "2008-01-01" @default.
- W1521054785 modified "2023-09-26" @default.
- W1521054785 title "Detection and Proteomic Identification of S‐Nitrosated Proteins in Human Hepatocytes" @default.
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- W1521054785 doi "https://doi.org/10.1016/s0076-6879(07)00817-8" @default.
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