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• W1521090160 abstract "We automate the manipulation of genomic-length DNA in a nanofluidic device based on real-time analysis of fluorescence images. In our protocol, individual molecules are picked from a microchannel and stretched with pN forces using pressure driven flows. The millimeter-long DNA fragments free flowing in micro- and nanofluidics emit low fluorescence and change shape, thus challenging the image analysis for machine vision. We demonstrate a set of image processing steps that increase the intrinsically low signal-to-noise ratio associated with single-molecule fluorescence microscopy. Furthermore, we demonstrate how to estimate the length of molecules by continuous real-time image stitching and how to increase the effective resolution of a pressure controller by pulse width modulation. The sequence of image-processing steps addresses the challenges of genomic-length DNA visualization; however, they should also be general to other applications of fluorescence-based microfluidics." @default.
• W1521090160 created "2016-06-24" @default.
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• W1521090160 date "2015-06-01" @default.
• W1521090160 modified "2023-09-23" @default.
• W1521090160 title "Automation of a single-DNA molecule stretching device" @default.
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• W1521090160 doi "https://doi.org/10.1063/1.4922068" @default.
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