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- W1521148011 abstract "An α- l -arabinofuranosidase (EC 3.2.1.55) was purified from the cytoplasm of Butyrivibrio fibrisolvens GS113. The native enzyme had an apparent molecular mass of 240 kDa and was composed of eight polypeptide subunits of 31 kDa. The enzyme displayed an isoelectric point of 6.0, a pH optimum of 6.0 to 6.5, a pH stability of 4.0 to 8.0, and a temperature optimum of 45°C and was stable to 55°C. The K m and V max for p -nitrophenyl-α- l -arabinofuranoside were 0.7 mM and 109 μmol/min/mg of protein, respectively. The enzyme was specific for the furanoside configuration and also readily cleaved methylumbelliferyl-α- l -arabinofuranoside but had no activity on a variety of other nitrophenyl- or methylumbelliferyl glycosides. When the enzyme was incubated with cellulose, carboxymethyl cellulose, or arabinogalactan, no release of sugars was found. Arabinose was found as the hydrolysis product of oatspelt xylan, corn endosperm xylan, or beet arabinan. No activity was detected when either coumaric or ferulic acid ester linked to arabinoxylobiose was used as substrates, but arabinoxylobiose was degraded to arabinose and xylobiose. Since B. fibrisolvens GS113 possesses essentially no extracellular arabinofuranosidase activity, the major role of the purified enzyme is apparently in the assimilation of arabinose-containing xylooligosaccharides generated from xylosidase, phenolic esterase, xylanase, and other enzymatic activities on xylans." @default.
- W1521148011 created "2016-06-24" @default.
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- W1521148011 date "1992-04-01" @default.
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- W1521148011 title "Purification and Characterization of an α- <scp>l</scp> -Arabinofuranosidase from <i>Butyrivibrio fibrisolvens</i> GS113" @default.
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- W1521148011 doi "https://doi.org/10.1128/aem.58.4.1082-1088.1992" @default.
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