FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521182579> ?p ?o ?g. }

• W1521182579 endingPage "317" @default.
• W1521182579 startingPage "308" @default.
• W1521182579 abstract "Catalysis by Escherichia coli inorganic pyrophosphatase (E-PPase) was found to be strongly modulated by Tris and similar aminoalcoholic buffers used in previous studies of this enzyme. By measuring ligand-binding and catalytic properties of E-PPase in zwitterionic buffers, we found that the previous data markedly underestimate Mg2+-binding affinity for two of the three sites present in E-PPase (3.5- to 16-fold) and the rate constant for substrate (dimagnesium pyrophosphate) binding to monomagnesium enzyme (20- to 40-fold). By contrast, Mg2+-binding and substrate conversion in the enzyme-substrate complex are unaffected by buffer. These data indicate that E-PPase requires in total only three Mg2+ ions per active site for best performance, rather than four, as previously believed. As measured by equilibrium dialysis, Mg2+ binds to 2.5 sites per monomer, supporting the notion that one of the tightly binding sites is located at the trimer–trimer interface. Mg2+ binding to the subunit interface site results in increased hexamer stability with only minor consequences for catalytic activity measured in the zwitterionic buffers, whereas Mg2+ binding to this site accelerates substrate binding up to 16-fold in the presence of Tris. Structural considerations favor the notion that the aminoalcohols bind to the E-PPase active site." @default.
• W1521182579 created "2016-06-24" @default.
• W1521182579 creator A5000742587 @default.
• W1521182579 creator A5010590439 @default.
• W1521182579 creator A5016178530 @default.
• W1521182579 creator A5043413256 @default.
• W1521182579 creator A5044235486 @default.
• W1521182579 creator A5064180492 @default.
• W1521182579 creator A5064957885 @default.
• W1521182579 creator A5074933688 @default.
• W1521182579 date "1999-03-01" @default.
• W1521182579 modified "2023-09-27" @default.
• W1521182579 title "Functional characterization ofEscherichia coliinorganic pyrophosphatase in zwitterionic buffers" @default.
• W1521182579 cites W1519899019 @default.
• W1521182579 cites W1522299751 @default.
• W1521182579 cites W1534879222 @default.
• W1521182579 cites W1559797399 @default.
• W1521182579 cites W1565345387 @default.
• W1521182579 cites W1877733339 @default.
• W1521182579 cites W1970174862 @default.
• W1521182579 cites W1973347443 @default.
• W1521182579 cites W1973521366 @default.
• W1521182579 cites W1979488636 @default.
• W1521182579 cites W1980252132 @default.
• W1521182579 cites W1984010593 @default.
• W1521182579 cites W1987559419 @default.
• W1521182579 cites W2001525539 @default.
• W1521182579 cites W2002772423 @default.
• W1521182579 cites W2005453049 @default.
• W1521182579 cites W2006640025 @default.
• W1521182579 cites W2021664722 @default.
• W1521182579 cites W2023653414 @default.
• W1521182579 cites W2024845076 @default.
• W1521182579 cites W2025070070 @default.
• W1521182579 cites W2025596570 @default.
• W1521182579 cites W2029634771 @default.
• W1521182579 cites W2039388758 @default.
• W1521182579 cites W2048363281 @default.
• W1521182579 cites W2050755167 @default.
• W1521182579 cites W2051224673 @default.
• W1521182579 cites W2057007659 @default.
• W1521182579 cites W2062769121 @default.
• W1521182579 cites W2068527810 @default.
• W1521182579 cites W2070852795 @default.
• W1521182579 cites W2072252524 @default.
• W1521182579 cites W2072343730 @default.
• W1521182579 cites W2072918486 @default.
• W1521182579 cites W2079368887 @default.
• W1521182579 cites W2085453602 @default.
• W1521182579 cites W2113914845 @default.
• W1521182579 cites W2119195950 @default.
• W1521182579 cites W2402108709 @default.
• W1521182579 doi "https://doi.org/10.1046/j.1432-1327.1999.00181.x" @default.
• W1521182579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10095764" @default.
• W1521182579 hasPublicationYear "1999" @default.
• W1521182579 type Work @default.
• W1521182579 sameAs 1521182579 @default.
• W1521182579 citedByCount "11" @default.
• W1521182579 countsByYear W15211825792013 @default.
• W1521182579 countsByYear W15211825792015 @default.
• W1521182579 countsByYear W15211825792021 @default.
• W1521182579 crossrefType "journal-article" @default.
• W1521182579 hasAuthorship W1521182579A5000742587 @default.
• W1521182579 hasAuthorship W1521182579A5010590439 @default.
• W1521182579 hasAuthorship W1521182579A5016178530 @default.
• W1521182579 hasAuthorship W1521182579A5043413256 @default.
• W1521182579 hasAuthorship W1521182579A5044235486 @default.
• W1521182579 hasAuthorship W1521182579A5064180492 @default.
• W1521182579 hasAuthorship W1521182579A5064957885 @default.
• W1521182579 hasAuthorship W1521182579A5074933688 @default.
• W1521182579 hasBestOaLocation W15211825791 @default.
• W1521182579 hasConcept C104292427 @default.
• W1521182579 hasConcept C104317684 @default.
• W1521182579 hasConcept C107824862 @default.
• W1521182579 hasConcept C116569031 @default.
• W1521182579 hasConcept C161790260 @default.
• W1521182579 hasConcept C170493617 @default.
• W1521182579 hasConcept C178790620 @default.
• W1521182579 hasConcept C181199279 @default.
• W1521182579 hasConcept C185592680 @default.
• W1521182579 hasConcept C18903297 @default.
• W1521182579 hasConcept C189754071 @default.
• W1521182579 hasConcept C2776043855 @default.
• W1521182579 hasConcept C2776403692 @default.
• W1521182579 hasConcept C2777008805 @default.
• W1521182579 hasConcept C2777289219 @default.
• W1521182579 hasConcept C2779546866 @default.
• W1521182579 hasConcept C2780091537 @default.
• W1521182579 hasConcept C2781014771 @default.
• W1521182579 hasConcept C41183919 @default.
• W1521182579 hasConcept C55493867 @default.
• W1521182579 hasConcept C71240020 @default.
• W1521182579 hasConcept C8010536 @default.
• W1521182579 hasConcept C86803240 @default.
• W1521182579 hasConceptScore W1521182579C104292427 @default.
• W1521182579 hasConceptScore W1521182579C104317684 @default.
• W1521182579 hasConceptScore W1521182579C107824862 @default.
• W1521182579 hasConceptScore W1521182579C116569031 @default.

• next