Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521291249> ?p ?o ?g. }
- W1521291249 endingPage "518" @default.
- W1521291249 startingPage "509" @default.
- W1521291249 abstract "Equilibrium dialysis and gel filtration studies show that tryptophanyl-tRNA synthetase from beef pancreas binds two molecules of l-tryptophan per dimer in an anticooperative way. The binding of tryptophan ellicits a series of spectroscopic changes in the protein as seen by absorbance, fluorescence and circular dichroism. The molar absorption change of the protein-tryp- tophan system upon formation of the complex is Δɛ292= 10400 ± 1000 M−1 cm−1 per dimer. Taking an initial symmetrical dimeric protein the two dissociation constants for tryptophan at pH 8, 25 C are respectively K1= 2.0 ± 0.5 μM and K2= 10 ± 4 μM. They are respectively K1= 1 ± 0.25 μM and K2= 20 ± 8 μM if one considers a sequenced binding of the two tryptophan molecules. The dichroic band at 290 nm of the free protein disappears when tryptophan is bound. All observed changes are characteristic of tryptophan perturbation and none of tyrosine perturbation. They all exceed the effect that can be expected from the change in environment of the bound tryptophan molecules and modifications of the tertiary structure of the protein have to be taken into account to explain the observed spectroscopic data." @default.
- W1521291249 created "2016-06-24" @default.
- W1521291249 creator A5027243343 @default.
- W1521291249 creator A5055712923 @default.
- W1521291249 creator A5058879598 @default.
- W1521291249 creator A5082614868 @default.
- W1521291249 creator A5086021159 @default.
- W1521291249 date "1979-06-01" @default.
- W1521291249 modified "2023-10-15" @default.
- W1521291249 title "Anticooperative Binding of l-Tryptophan to Tryptophanyl-tRNA Synthetase from Beef Pancreas. Study at Equilibrium by Dialysis and Changes in Spectroscopic Properties" @default.
- W1521291249 cites W1484289460 @default.
- W1521291249 cites W1494984572 @default.
- W1521291249 cites W1499805402 @default.
- W1521291249 cites W1513843435 @default.
- W1521291249 cites W1514860801 @default.
- W1521291249 cites W1524954922 @default.
- W1521291249 cites W1556499851 @default.
- W1521291249 cites W1562594164 @default.
- W1521291249 cites W1583519957 @default.
- W1521291249 cites W1590627095 @default.
- W1521291249 cites W1659843151 @default.
- W1521291249 cites W1773429629 @default.
- W1521291249 cites W1969491840 @default.
- W1521291249 cites W1972165019 @default.
- W1521291249 cites W1985045851 @default.
- W1521291249 cites W1985490709 @default.
- W1521291249 cites W1993442337 @default.
- W1521291249 cites W1997375770 @default.
- W1521291249 cites W2002712489 @default.
- W1521291249 cites W2003869975 @default.
- W1521291249 cites W2005700051 @default.
- W1521291249 cites W2012045759 @default.
- W1521291249 cites W2012354655 @default.
- W1521291249 cites W2014550271 @default.
- W1521291249 cites W2015287678 @default.
- W1521291249 cites W2016579798 @default.
- W1521291249 cites W2020270026 @default.
- W1521291249 cites W2023079428 @default.
- W1521291249 cites W2026912546 @default.
- W1521291249 cites W2041184225 @default.
- W1521291249 cites W2050511169 @default.
- W1521291249 cites W2055380830 @default.
- W1521291249 cites W2061734875 @default.
- W1521291249 cites W2061901997 @default.
- W1521291249 cites W2062153188 @default.
- W1521291249 cites W2076982321 @default.
- W1521291249 cites W2084712516 @default.
- W1521291249 cites W2087422806 @default.
- W1521291249 cites W2112143239 @default.
- W1521291249 cites W2209250776 @default.
- W1521291249 cites W2315207049 @default.
- W1521291249 cites W595624220 @default.
- W1521291249 doi "https://doi.org/10.1111/j.1432-1033.1979.tb13064.x" @default.
- W1521291249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/467418" @default.
- W1521291249 hasPublicationYear "1979" @default.
- W1521291249 type Work @default.
- W1521291249 sameAs 1521291249 @default.
- W1521291249 citedByCount "16" @default.
- W1521291249 countsByYear W15212912492018 @default.
- W1521291249 countsByYear W15212912492019 @default.
- W1521291249 countsByYear W15212912492021 @default.
- W1521291249 crossrefType "journal-article" @default.
- W1521291249 hasAuthorship W1521291249A5027243343 @default.
- W1521291249 hasAuthorship W1521291249A5055712923 @default.
- W1521291249 hasAuthorship W1521291249A5058879598 @default.
- W1521291249 hasAuthorship W1521291249A5082614868 @default.
- W1521291249 hasAuthorship W1521291249A5086021159 @default.
- W1521291249 hasConcept C121332964 @default.
- W1521291249 hasConcept C133571119 @default.
- W1521291249 hasConcept C170493617 @default.
- W1521291249 hasConcept C178790620 @default.
- W1521291249 hasConcept C185592680 @default.
- W1521291249 hasConcept C2776165026 @default.
- W1521291249 hasConcept C2776706248 @default.
- W1521291249 hasConcept C2777503454 @default.
- W1521291249 hasConcept C2779546866 @default.
- W1521291249 hasConcept C32909587 @default.
- W1521291249 hasConcept C43617362 @default.
- W1521291249 hasConcept C515207424 @default.
- W1521291249 hasConcept C55493867 @default.
- W1521291249 hasConcept C62520636 @default.
- W1521291249 hasConcept C71240020 @default.
- W1521291249 hasConcept C74998103 @default.
- W1521291249 hasConcept C8010536 @default.
- W1521291249 hasConcept C91881484 @default.
- W1521291249 hasConcept C98015330 @default.
- W1521291249 hasConceptScore W1521291249C121332964 @default.
- W1521291249 hasConceptScore W1521291249C133571119 @default.
- W1521291249 hasConceptScore W1521291249C170493617 @default.
- W1521291249 hasConceptScore W1521291249C178790620 @default.
- W1521291249 hasConceptScore W1521291249C185592680 @default.
- W1521291249 hasConceptScore W1521291249C2776165026 @default.
- W1521291249 hasConceptScore W1521291249C2776706248 @default.
- W1521291249 hasConceptScore W1521291249C2777503454 @default.
- W1521291249 hasConceptScore W1521291249C2779546866 @default.
- W1521291249 hasConceptScore W1521291249C32909587 @default.
- W1521291249 hasConceptScore W1521291249C43617362 @default.
- W1521291249 hasConceptScore W1521291249C515207424 @default.