Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521585242> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1521585242 endingPage "8873" @default.
- W1521585242 startingPage "8869" @default.
- W1521585242 abstract "Synaptophysin, a major membrane protein of synaptic vesicles, contains four transmembrane regions and two intravesicular loops. Synaptophysin monomers associate into homopolymers that have the potential to form channels in the synaptic vesicle membrane. Here we show that in native synaptophysin, homopolymers are linked by noncovalent forces. The molecule contains unstable intramolecular disulfide bonds that undergo disulfide exchange during solubilization, thereby covalently cross-linking neighboring synaptophysin molecules. The locations of the intramolecular disulfide bonds in synaptophysin were determined, revealing that each of the two intravesicular loops of synaptophysin is circularized by a single disulfide bond. Cross-linking of synaptophysin by disulfide bonds can be triggered in synaptic vesicles and in intact cells by a cycle of reduction and oxidation, suggesting that native synaptophysin is a homomultimer in situ. In addition, chemical cross-linking of native synaptophysin demonstrates that a low molecular weight protein is specifically associated with synaptophysin complexes and is lost upon reduction of the intramolecular disulfide bonds. These data suggest that native synaptophysin forms a noncovalent homomultimeric complex whose structure and interaction with other proteins are dependent on the integrity of its intramolecular disulfide bonds and phospholipid environment." @default.
- W1521585242 created "2016-06-24" @default.
- W1521585242 creator A5061921730 @default.
- W1521585242 creator A5071284319 @default.
- W1521585242 date "1990-05-01" @default.
- W1521585242 modified "2023-09-28" @default.
- W1521585242 title "The multisubunit structure of synaptophysin. Relationship between disulfide bonding and homo-oligomerization." @default.
- W1521585242 cites W11429024 @default.
- W1521585242 cites W1213121153 @default.
- W1521585242 cites W1535011143 @default.
- W1521585242 cites W1846181097 @default.
- W1521585242 cites W1987357993 @default.
- W1521585242 cites W1996241166 @default.
- W1521585242 cites W1996573332 @default.
- W1521585242 cites W2000404687 @default.
- W1521585242 cites W2010313687 @default.
- W1521585242 cites W2014045979 @default.
- W1521585242 cites W2019156595 @default.
- W1521585242 cites W2021269348 @default.
- W1521585242 cites W2034886207 @default.
- W1521585242 cites W2042714786 @default.
- W1521585242 cites W2048019766 @default.
- W1521585242 cites W2064148637 @default.
- W1521585242 cites W2089333920 @default.
- W1521585242 cites W2100837269 @default.
- W1521585242 cites W2101108802 @default.
- W1521585242 cites W2145819351 @default.
- W1521585242 cites W2164220708 @default.
- W1521585242 cites W2237496182 @default.
- W1521585242 cites W4293247451 @default.
- W1521585242 doi "https://doi.org/10.1016/s0021-9258(19)38968-9" @default.
- W1521585242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2111326" @default.
- W1521585242 hasPublicationYear "1990" @default.
- W1521585242 type Work @default.
- W1521585242 sameAs 1521585242 @default.
- W1521585242 citedByCount "95" @default.
- W1521585242 countsByYear W15215852422013 @default.
- W1521585242 countsByYear W15215852422014 @default.
- W1521585242 countsByYear W15215852422016 @default.
- W1521585242 countsByYear W15215852422017 @default.
- W1521585242 countsByYear W15215852422020 @default.
- W1521585242 countsByYear W15215852422021 @default.
- W1521585242 countsByYear W15215852422022 @default.
- W1521585242 crossrefType "journal-article" @default.
- W1521585242 hasAuthorship W1521585242A5061921730 @default.
- W1521585242 hasAuthorship W1521585242A5071284319 @default.
- W1521585242 hasBestOaLocation W15215852421 @default.
- W1521585242 hasConcept C12554922 @default.
- W1521585242 hasConcept C126322002 @default.
- W1521585242 hasConcept C185592680 @default.
- W1521585242 hasConcept C204232928 @default.
- W1521585242 hasConcept C27256138 @default.
- W1521585242 hasConcept C2777209535 @default.
- W1521585242 hasConcept C55493867 @default.
- W1521585242 hasConcept C71924100 @default.
- W1521585242 hasConcept C86803240 @default.
- W1521585242 hasConceptScore W1521585242C12554922 @default.
- W1521585242 hasConceptScore W1521585242C126322002 @default.
- W1521585242 hasConceptScore W1521585242C185592680 @default.
- W1521585242 hasConceptScore W1521585242C204232928 @default.
- W1521585242 hasConceptScore W1521585242C27256138 @default.
- W1521585242 hasConceptScore W1521585242C2777209535 @default.
- W1521585242 hasConceptScore W1521585242C55493867 @default.
- W1521585242 hasConceptScore W1521585242C71924100 @default.
- W1521585242 hasConceptScore W1521585242C86803240 @default.
- W1521585242 hasIssue "15" @default.
- W1521585242 hasLocation W15215852421 @default.
- W1521585242 hasOpenAccess W1521585242 @default.
- W1521585242 hasPrimaryLocation W15215852421 @default.
- W1521585242 hasRelatedWork W1521585242 @default.
- W1521585242 hasRelatedWork W1982843405 @default.
- W1521585242 hasRelatedWork W2045355728 @default.
- W1521585242 hasRelatedWork W2060268288 @default.
- W1521585242 hasRelatedWork W2329655268 @default.
- W1521585242 hasRelatedWork W2333726361 @default.
- W1521585242 hasRelatedWork W2341499342 @default.
- W1521585242 hasRelatedWork W2805287293 @default.
- W1521585242 hasRelatedWork W2950785785 @default.
- W1521585242 hasRelatedWork W2951715315 @default.
- W1521585242 hasVolume "265" @default.
- W1521585242 isParatext "false" @default.
- W1521585242 isRetracted "false" @default.
- W1521585242 magId "1521585242" @default.
- W1521585242 workType "article" @default.