Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521640184> ?p ?o ?g. }
- W1521640184 endingPage "21273" @default.
- W1521640184 startingPage "21265" @default.
- W1521640184 abstract "We have generated and characterized three rabbit polyclonal antibodies that recognize different regions of the epidermal growth factor receptor (EGF-R) and used them to study the degradation of the receptor in the isolated perfused rat liver. Quantitative immunoblot analyses of rat liver homogenates prepared from tissue biopsies collected at various times after epidermal growth factor (EGF) addition showed that both the ectoplasmic and cytoplasmic domains of rat liver EGF-Rs were degraded with similar kinetics (t1/2 = 3.5-3.8 h at 25 degrees C with cycloheximide). No immunoreactive intermediate breakdown products were detected. EGF-stimulated degradation of both receptor domains was inhibited by the thiol protease inhibitor leupeptin, suggesting lysosome involvement in the hydrolysis of the whole molecule. To study this further, protease protection experiments were performed on endosome- and lysosome-enriched fractions isolated from leupeptin-treated livers. We found that the cytoplasmic domains of greater than 90% of the EGF-Rs in endosomal fractions were accessible to digestion when proteinase K was added to the intact vesicle populations, while the ectoplasmic domain was unaltered. In contrast, both the ectoplasmic and cytoplasmic domains of approximately 55% of the EGF-Rs present in lysosome-enriched fractions were inaccessible to proteinase K digestion in the absence of detergent. These findings suggest that movement of EGF-Rs from the limiting membrane of endosomes to the lumen of lysosomes permits the degradation of the entire EGF-R molecule within lysosomes." @default.
- W1521640184 created "2016-06-24" @default.
- W1521640184 creator A5045164176 @default.
- W1521640184 creator A5066037259 @default.
- W1521640184 date "1991-11-01" @default.
- W1521640184 modified "2023-10-09" @default.
- W1521640184 title "Degradation of epidermal growth factor receptor in rat liver. Membrane topology through the lysosomal pathway." @default.
- W1521640184 cites W1471178508 @default.
- W1521640184 cites W1497356015 @default.
- W1521640184 cites W1498071142 @default.
- W1521640184 cites W1512047600 @default.
- W1521640184 cites W1518877053 @default.
- W1521640184 cites W1529786888 @default.
- W1521640184 cites W1535903873 @default.
- W1521640184 cites W1541458650 @default.
- W1521640184 cites W1556750259 @default.
- W1521640184 cites W1569697157 @default.
- W1521640184 cites W1573437712 @default.
- W1521640184 cites W1577788712 @default.
- W1521640184 cites W1581595804 @default.
- W1521640184 cites W1584694243 @default.
- W1521640184 cites W1775749144 @default.
- W1521640184 cites W1780481074 @default.
- W1521640184 cites W1893651774 @default.
- W1521640184 cites W1969438 @default.
- W1521640184 cites W1981345384 @default.
- W1521640184 cites W1984351623 @default.
- W1521640184 cites W1986807124 @default.
- W1521640184 cites W1995135269 @default.
- W1521640184 cites W1995337883 @default.
- W1521640184 cites W1995574514 @default.
- W1521640184 cites W2013073732 @default.
- W1521640184 cites W2017641062 @default.
- W1521640184 cites W2020072455 @default.
- W1521640184 cites W2021752338 @default.
- W1521640184 cites W2032359283 @default.
- W1521640184 cites W2032422012 @default.
- W1521640184 cites W2032559513 @default.
- W1521640184 cites W2042262755 @default.
- W1521640184 cites W2044727795 @default.
- W1521640184 cites W2068443270 @default.
- W1521640184 cites W2069314886 @default.
- W1521640184 cites W2075846958 @default.
- W1521640184 cites W2078494160 @default.
- W1521640184 cites W2080964580 @default.
- W1521640184 cites W2086271423 @default.
- W1521640184 cites W2090784961 @default.
- W1521640184 cites W2103174300 @default.
- W1521640184 cites W2106098936 @default.
- W1521640184 cites W2140093238 @default.
- W1521640184 cites W2146447599 @default.
- W1521640184 cites W2159153404 @default.
- W1521640184 cites W2161163260 @default.
- W1521640184 cites W2400404181 @default.
- W1521640184 cites W2922246927 @default.
- W1521640184 cites W76272849 @default.
- W1521640184 cites W79821297 @default.
- W1521640184 doi "https://doi.org/10.1016/s0021-9258(18)54850-x" @default.
- W1521640184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1682317" @default.
- W1521640184 hasPublicationYear "1991" @default.
- W1521640184 type Work @default.
- W1521640184 sameAs 1521640184 @default.
- W1521640184 citedByCount "54" @default.
- W1521640184 countsByYear W15216401842015 @default.
- W1521640184 countsByYear W15216401842016 @default.
- W1521640184 countsByYear W15216401842022 @default.
- W1521640184 crossrefType "journal-article" @default.
- W1521640184 hasAuthorship W1521640184A5045164176 @default.
- W1521640184 hasAuthorship W1521640184A5066037259 @default.
- W1521640184 hasBestOaLocation W15216401841 @default.
- W1521640184 hasConcept C114614502 @default.
- W1521640184 hasConcept C12554922 @default.
- W1521640184 hasConcept C170493617 @default.
- W1521640184 hasConcept C184720557 @default.
- W1521640184 hasConcept C185592680 @default.
- W1521640184 hasConcept C2776362946 @default.
- W1521640184 hasConcept C2779438470 @default.
- W1521640184 hasConcept C2779679103 @default.
- W1521640184 hasConcept C33923547 @default.
- W1521640184 hasConcept C41008148 @default.
- W1521640184 hasConcept C55493867 @default.
- W1521640184 hasConcept C76155785 @default.
- W1521640184 hasConcept C86803240 @default.
- W1521640184 hasConcept C95444343 @default.
- W1521640184 hasConceptScore W1521640184C114614502 @default.
- W1521640184 hasConceptScore W1521640184C12554922 @default.
- W1521640184 hasConceptScore W1521640184C170493617 @default.
- W1521640184 hasConceptScore W1521640184C184720557 @default.
- W1521640184 hasConceptScore W1521640184C185592680 @default.
- W1521640184 hasConceptScore W1521640184C2776362946 @default.
- W1521640184 hasConceptScore W1521640184C2779438470 @default.
- W1521640184 hasConceptScore W1521640184C2779679103 @default.
- W1521640184 hasConceptScore W1521640184C33923547 @default.
- W1521640184 hasConceptScore W1521640184C41008148 @default.
- W1521640184 hasConceptScore W1521640184C55493867 @default.
- W1521640184 hasConceptScore W1521640184C76155785 @default.
- W1521640184 hasConceptScore W1521640184C86803240 @default.
- W1521640184 hasConceptScore W1521640184C95444343 @default.