FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521822265> ?p ?o ?g. }

• W1521822265 endingPage "7556" @default.
• W1521822265 startingPage "7542" @default.
• W1521822265 abstract "Group B Streptococcus (GBS) has developed several strategies to evade immune defenses. We show that GBS induces macrophage (Mphi) membrane permeability defects and apoptosis, prevented by inhibition of calcium influx but not caspases. We analyze the molecular mechanisms of GBS-induced murine Mphi apoptosis. GBS causes a massive intracellular calcium increase, strictly correlated to membrane permeability defects and apoptosis onset. Calcium increase was associated with activation of calcium-dependent protease calpain, demonstrated by casein zymography, alpha-spectrin cleavage to a calpain-specific fragment, fluorogenic calpain-substrate cleavage, and inhibition of these proteolyses by calpain inhibitors targeting the calcium-binding, 3-(4-Iodophenyl)-2-mercapto-(Z)-2-propenoic acid, or active site (four different inhibitors), by calpain small-interfering-RNA (siRNA) and EGTA. GBS-induced Mphi apoptosis was inhibited by all micro- and m-calpain inhibitors used and m-calpain siRNA, but not 3-(5-Fluoro-3-indolyl)-2-mercapto-(Z)-2-propenoic acid (micro-calpain inhibitor) and micro-calpain siRNA indicating that m-calpain plays a central role in apoptosis. Calpain activation is followed by Bax and Bid cleavage, cytochrome c, apoptosis-inducing factor, and endonuclease G release from mitochondria. In GBS-induced apoptosis, cytochrome c did not induce caspase-3 and -7 activation because they and APAF-1 were degraded by calpains. Therefore, apoptosis-inducing factor and endonuclease G seem the main mediators of the calpain-dependent but caspase-independent pathway of GBS-induced apoptosis. Proapoptotic mediator degradations do not occur with nonhemolytic GBS, not inducing Mphi apoptosis. Apoptosis was reduced by Bax siRNA and Bid siRNA suggesting Bax and Bid degradation is apoptosis correlated. This signaling pathway, different from that of most pathogens, could represent a GBS strategy to evade immune defenses." @default.
• W1521822265 created "2016-06-24" @default.
• W1521822265 creator A5000627591 @default.
• W1521822265 creator A5007263818 @default.
• W1521822265 creator A5039677890 @default.
• W1521822265 creator A5043901809 @default.
• W1521822265 creator A5061990903 @default.
• W1521822265 creator A5076906237 @default.
• W1521822265 creator A5091170737 @default.
• W1521822265 date "2006-06-15" @default.
• W1521822265 modified "2023-10-16" @default.
• W1521822265 title "Group B <i>Streptococcus</i> Induces Macrophage Apoptosis by Calpain Activation" @default.
• W1521822265 cites W1524835356 @default.
• W1521822265 cites W1544689824 @default.
• W1521822265 cites W1544768334 @default.
• W1521822265 cites W1602075904 @default.
• W1521822265 cites W1611419331 @default.
• W1521822265 cites W1790840706 @default.
• W1521822265 cites W1904882776 @default.
• W1521822265 cites W1959169074 @default.
• W1521822265 cites W1972636570 @default.
• W1521822265 cites W1977644290 @default.
• W1521822265 cites W1981579162 @default.
• W1521822265 cites W1984369710 @default.
• W1521822265 cites W1985260732 @default.
• W1521822265 cites W1987945908 @default.
• W1521822265 cites W1988433390 @default.
• W1521822265 cites W1988728998 @default.
• W1521822265 cites W1990019612 @default.
• W1521822265 cites W1993331127 @default.
• W1521822265 cites W2002208801 @default.
• W1521822265 cites W2002926498 @default.
• W1521822265 cites W2014101818 @default.
• W1521822265 cites W2016133409 @default.
• W1521822265 cites W2021658884 @default.
• W1521822265 cites W2025134285 @default.
• W1521822265 cites W2029797799 @default.
• W1521822265 cites W2034081389 @default.
• W1521822265 cites W2038933175 @default.
• W1521822265 cites W2039924353 @default.
• W1521822265 cites W2045267492 @default.
• W1521822265 cites W2049427398 @default.
• W1521822265 cites W2054319300 @default.
• W1521822265 cites W2060184827 @default.
• W1521822265 cites W2060725618 @default.
• W1521822265 cites W2061690334 @default.
• W1521822265 cites W2064315633 @default.
• W1521822265 cites W2064866491 @default.
• W1521822265 cites W2065416929 @default.
• W1521822265 cites W2066204502 @default.
• W1521822265 cites W2072180708 @default.
• W1521822265 cites W2085414692 @default.
• W1521822265 cites W2085942153 @default.
• W1521822265 cites W2087261949 @default.
• W1521822265 cites W2090137644 @default.
• W1521822265 cites W2099276474 @default.
• W1521822265 cites W2106289607 @default.
• W1521822265 cites W2107235611 @default.
• W1521822265 cites W2120181693 @default.
• W1521822265 cites W2121698356 @default.
• W1521822265 cites W2123455365 @default.
• W1521822265 cites W2124077788 @default.
• W1521822265 cites W2126456566 @default.
• W1521822265 cites W2134026439 @default.
• W1521822265 cites W2134158036 @default.
• W1521822265 cites W2135298969 @default.
• W1521822265 cites W2136765431 @default.
• W1521822265 cites W2146582952 @default.
• W1521822265 cites W2149023741 @default.
• W1521822265 cites W2149757052 @default.
• W1521822265 cites W2151501496 @default.
• W1521822265 cites W2153362338 @default.
• W1521822265 cites W2155756489 @default.
• W1521822265 cites W2155775702 @default.
• W1521822265 cites W2164578725 @default.
• W1521822265 cites W2169349825 @default.
• W1521822265 cites W2170107782 @default.
• W1521822265 cites W2190670127 @default.
• W1521822265 cites W2398657384 @default.
• W1521822265 cites W4239718055 @default.
• W1521822265 cites W4244332054 @default.
• W1521822265 cites W7933831 @default.
• W1521822265 doi "https://doi.org/10.4049/jimmunol.176.12.7542" @default.
• W1521822265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16751401" @default.
• W1521822265 hasPublicationYear "2006" @default.
• W1521822265 type Work @default.
• W1521822265 sameAs 1521822265 @default.
• W1521822265 citedByCount "68" @default.
• W1521822265 countsByYear W15218222652012 @default.
• W1521822265 countsByYear W15218222652013 @default.
• W1521822265 countsByYear W15218222652014 @default.
• W1521822265 countsByYear W15218222652015 @default.
• W1521822265 countsByYear W15218222652016 @default.
• W1521822265 countsByYear W15218222652017 @default.
• W1521822265 countsByYear W15218222652018 @default.
• W1521822265 countsByYear W15218222652022 @default.
• W1521822265 countsByYear W15218222652023 @default.
• W1521822265 crossrefType "journal-article" @default.

• next