Matches in SemOpenAlex for { <https://semopenalex.org/work/W1521897480> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1521897480 endingPage "8487" @default.
- W1521897480 startingPage "8483" @default.
- W1521897480 abstract "The receptor for human interferon-gamma (IFN-gamma) was purified from foreskin fibroblasts. Triton X-100 extracts obtained from either intact cells or membrane preparations were passed through an immobilized interferon-gamma column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of eluted fractions revealed a major band of Mr = 95,000 and minor bands of Mr = 80,000 and 60,000. Further purification was obtained by steric exclusion and by lectin chromatography. The purified receptor retained the ability to bind 125I-IFN-gamma with a Kd of 2.2 X 10(-10) M, a value close to that obtained with intact fibroblasts (5 X 10(-10) M). A complex of Mr = 105,000-125,000 was visualized by immunoprecipitation of 125I-IFN-gamma cross-linked to the purified receptor followed by SDS-PAGE and autoradiography. A similar complex was obtained when 125I-IFN-gamma was cross-linked to intact cells. Immunization of mice with the excised SDS-PAGE band of Mr = 95,000 elicited antibodies that blocked the antiviral activity of IFN-gamma and immunoprecipitated the cross-linked complex of 125I-IFN-gamma and its receptor." @default.
- W1521897480 created "2016-06-24" @default.
- W1521897480 creator A5004423788 @default.
- W1521897480 creator A5004647809 @default.
- W1521897480 creator A5010061511 @default.
- W1521897480 creator A5074793894 @default.
- W1521897480 date "1987-06-01" @default.
- W1521897480 modified "2023-10-18" @default.
- W1521897480 title "The human interferon-gamma receptor. Purification, characterization, and preparation of antibodies." @default.
- W1521897480 cites W1422881997 @default.
- W1521897480 cites W1483645952 @default.
- W1521897480 cites W1490800426 @default.
- W1521897480 cites W1542531015 @default.
- W1521897480 cites W1550576044 @default.
- W1521897480 cites W1550814250 @default.
- W1521897480 cites W1558985378 @default.
- W1521897480 cites W1567059382 @default.
- W1521897480 cites W1589391550 @default.
- W1521897480 cites W1975676125 @default.
- W1521897480 cites W1982093100 @default.
- W1521897480 cites W2016540239 @default.
- W1521897480 cites W2022961230 @default.
- W1521897480 cites W2043813981 @default.
- W1521897480 cites W2061817244 @default.
- W1521897480 cites W2061883989 @default.
- W1521897480 cites W2068472838 @default.
- W1521897480 cites W2072522791 @default.
- W1521897480 cites W2100837269 @default.
- W1521897480 cites W258376682 @default.
- W1521897480 doi "https://doi.org/10.1016/s0021-9258(18)47439-x" @default.
- W1521897480 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2954953" @default.
- W1521897480 hasPublicationYear "1987" @default.
- W1521897480 type Work @default.
- W1521897480 sameAs 1521897480 @default.
- W1521897480 citedByCount "75" @default.
- W1521897480 countsByYear W15218974802013 @default.
- W1521897480 countsByYear W15218974802014 @default.
- W1521897480 countsByYear W15218974802015 @default.
- W1521897480 countsByYear W15218974802017 @default.
- W1521897480 countsByYear W15218974802019 @default.
- W1521897480 countsByYear W15218974802022 @default.
- W1521897480 countsByYear W15218974802023 @default.
- W1521897480 crossrefType "journal-article" @default.
- W1521897480 hasAuthorship W1521897480A5004423788 @default.
- W1521897480 hasAuthorship W1521897480A5004647809 @default.
- W1521897480 hasAuthorship W1521897480A5010061511 @default.
- W1521897480 hasAuthorship W1521897480A5074793894 @default.
- W1521897480 hasBestOaLocation W15218974801 @default.
- W1521897480 hasConcept C153911025 @default.
- W1521897480 hasConcept C159654299 @default.
- W1521897480 hasConcept C170493617 @default.
- W1521897480 hasConcept C185592680 @default.
- W1521897480 hasConcept C202751555 @default.
- W1521897480 hasConcept C203014093 @default.
- W1521897480 hasConcept C2780841837 @default.
- W1521897480 hasConcept C55493867 @default.
- W1521897480 hasConcept C86803240 @default.
- W1521897480 hasConceptScore W1521897480C153911025 @default.
- W1521897480 hasConceptScore W1521897480C159654299 @default.
- W1521897480 hasConceptScore W1521897480C170493617 @default.
- W1521897480 hasConceptScore W1521897480C185592680 @default.
- W1521897480 hasConceptScore W1521897480C202751555 @default.
- W1521897480 hasConceptScore W1521897480C203014093 @default.
- W1521897480 hasConceptScore W1521897480C2780841837 @default.
- W1521897480 hasConceptScore W1521897480C55493867 @default.
- W1521897480 hasConceptScore W1521897480C86803240 @default.
- W1521897480 hasIssue "18" @default.
- W1521897480 hasLocation W15218974801 @default.
- W1521897480 hasOpenAccess W1521897480 @default.
- W1521897480 hasPrimaryLocation W15218974801 @default.
- W1521897480 hasRelatedWork W15021325 @default.
- W1521897480 hasRelatedWork W1933900219 @default.
- W1521897480 hasRelatedWork W1985432028 @default.
- W1521897480 hasRelatedWork W1986249076 @default.
- W1521897480 hasRelatedWork W2010808173 @default.
- W1521897480 hasRelatedWork W2059391185 @default.
- W1521897480 hasRelatedWork W2074785916 @default.
- W1521897480 hasRelatedWork W2108895381 @default.
- W1521897480 hasRelatedWork W2188270127 @default.
- W1521897480 hasRelatedWork W2985048223 @default.
- W1521897480 hasVolume "262" @default.
- W1521897480 isParatext "false" @default.
- W1521897480 isRetracted "false" @default.
- W1521897480 magId "1521897480" @default.
- W1521897480 workType "article" @default.