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- W152194847 abstract "A feasibility study was planned to determine the efficiency of immobilized algal cells growing in a packed bed for removing Cd from commercial effluents. To select appropriate material for an immobilized cell system, twenty-five strains of algae isolated from heavy-metal contaminated environments of known water chemistry were tested for their ability to accumulate Cd. Before accumulation experiments were initiated, ion exchange resin was employed to demonstrate that EDTA in the medium did not complex Cd to a significant degree. Svnechococcus D562 cells subcultured in Cd accumulated the most metal; little was bound to the cell wall. A continuous culture of steady- state Svnechococcus D562 cells tolerated a lower maximum concentration of metal (3.4 mg 1(^-1) Cd) than batch- cultured cells (5 mg 1(^-1) Cd), indicating that metabolic status influences the toxicity of Cd. When flasks of calcium-alginate beads were challenged with Cd, up to 60 % of the added metal was bound within 16 h; however, further incubation did not reduce the pollutant concentration. Two axenic strains which accumulated the metal to a high concentration were then immobilized and tested for their capacity to remove Cd from the circulating medium. A packed-bed reactor containing Mougeotia D536 cells proved more effective at metal removal than Svnechococcus D562, but both species grew to a lower cell density at the effluent end of the column. The medium was then aerated to overcome such growth-limiting conditions, but this treatment inhibited Cd accumulation. Column-immobilized cells reduced Cd levels more effectively than inoculated, alginate beads in stationary flasks or free cells. Energy dispersive X-ray microanalysis located Cd only in particular Svnechococcus D562 polyphosphate bodies (those with a high Ca to K ratio); peaks for Zn, Pb, Fe, Mn and Ba were also detected in algae isolated from the field. Scanning proton microanalysis provided information on the distribution of macro- and micro-elements throughout the two strains of cyanobacteria and two strains of algae selected from the Durham Culture Collection and demonstrated the presence of Cd in Klebsormidium rivulare D537.Detergent-sensitive spheroplasts of Svnechococcus D562 were produced by lysozyme and protease digestion, but were not viable for growth. To observe the extracellular mucilage of this strain by EM, lysozyme digestion proved imperative for effective ruthenium red staining to convert the material into an electron opaque material. From cultures of Svnechococcus D562 grown with or without Cd a 14 kD plasmid was isolated, which contained two Eco RI, two Bam HI and five Hind III restriction sites. A radiolabelled oligonucleotide probe based on part of the nucleotide sequence of a metallothionein from Svnechococcus FCC 6301 did not bind to a genomic and plasmid blot of Svnechococcus D562 DNA. The putative Cd-binding peptides ((yEC)(_n)G's) that were discovered only bound significant quantities of the metal when cells were exposed to 6.17 mg 1(^-1) Cd for 2 days at the end of their log-growth phase. Indigenous peptides failed to bind substantial amounts of the metal and the presence of Cd throughout growth did not influence the quantity of chelated Cd, except for Mougeotia D536. The pH of half displacement for (yEQjp's from this strain is comparable with that of other species. Reversed-phase HPLC of the peptides from Mougeotia D536 generated a thiol profile similar to that recorded for the Cd-binding peptides of Datura innoxia. The Cd-induced ultrastructural distortions that were recorded include potential Ca / P / Cd precipitates in Mougeotia D536, the loss of polyglucoside granules from Calothrix D184 together with a relaxation of its thylakoid packing and a lack of plastoglobuli in Cd-exposed Klebsormidium D537. The space between an immobilized cell and the matrix either represents shrinkage of the matrix during dehydration or mucilage which does not bind electron dense stains. Release of alkaline phosphatase into the medium by Svnechococcus D562, provided suitable material to study the inhibitory effects of Cd upon P hydrolysis. Ultrafiltration membranes proved effective as initial step towards enzyme purification and for the determination of activity under sub-optimal pH conditions. At pH 7.0, the activity of an enzyme concentrate was inhibited when 1 and 10 mg 1(^-1) Cd were added to the assay medium, but the presence of this metal in the growth medium did not reduce activity. One-dimensional SDS PAGE revealed only one protein difference between strains grown with or without Cd; a reduction in the staining intensity of a 17 kD band of Calothrix D184." @default.
- W152194847 created "2016-06-24" @default.
- W152194847 creator A5012718620 @default.
- W152194847 date "1990-01-01" @default.
- W152194847 modified "2023-09-28" @default.
- W152194847 title "Removal of cadmium from polluted water by immobilized algae" @default.
- W152194847 cites W13368843 @default.
- W152194847 cites W139886774 @default.
- W152194847 cites W1490983938 @default.
- W152194847 cites W1492210600 @default.
- W152194847 cites W1500830246 @default.
- W152194847 cites W1552759476 @default.
- W152194847 cites W1559244050 @default.
- W152194847 cites W1560709946 @default.
- W152194847 cites W1562302722 @default.
- W152194847 cites W1563698749 @default.
- W152194847 cites W1564619302 @default.
- W152194847 cites W1646495957 @default.
- W152194847 cites W1679286551 @default.
- W152194847 cites W1711178672 @default.
- W152194847 cites W1775077782 @default.
- W152194847 cites W1791848758 @default.
- W152194847 cites W1814790092 @default.
- W152194847 cites W1888692686 @default.
- W152194847 cites W1896918741 @default.
- W152194847 cites W191321353 @default.
- W152194847 cites W1965774837 @default.
- W152194847 cites W1967024955 @default.
- W152194847 cites W1970394801 @default.
- W152194847 cites W1974382441 @default.
- W152194847 cites W1974648961 @default.
- W152194847 cites W1974707342 @default.
- W152194847 cites W1975885409 @default.
- W152194847 cites W1980834452 @default.
- W152194847 cites W1981256927 @default.
- W152194847 cites W1981639885 @default.
- W152194847 cites W1982399036 @default.
- W152194847 cites W1982632503 @default.
- W152194847 cites W1984582011 @default.
- W152194847 cites W1985735718 @default.
- W152194847 cites W1986319213 @default.
- W152194847 cites W1986796571 @default.
- W152194847 cites W1987774904 @default.
- W152194847 cites W1988087083 @default.
- W152194847 cites W1988101526 @default.
- W152194847 cites W1989724894 @default.
- W152194847 cites W1992867047 @default.
- W152194847 cites W1998770312 @default.
- W152194847 cites W1999257590 @default.
- W152194847 cites W2000141775 @default.
- W152194847 cites W2000589267 @default.
- W152194847 cites W2000625981 @default.
- W152194847 cites W2002404337 @default.
- W152194847 cites W2003202698 @default.
- W152194847 cites W2003967355 @default.
- W152194847 cites W2004037332 @default.
- W152194847 cites W2005596964 @default.
- W152194847 cites W2006370393 @default.
- W152194847 cites W2006580156 @default.
- W152194847 cites W2006590379 @default.
- W152194847 cites W2015134115 @default.
- W152194847 cites W2015951770 @default.
- W152194847 cites W2018150931 @default.
- W152194847 cites W2018666462 @default.
- W152194847 cites W2020684948 @default.
- W152194847 cites W2023777540 @default.
- W152194847 cites W2025379350 @default.
- W152194847 cites W2025697141 @default.
- W152194847 cites W2036955578 @default.
- W152194847 cites W2039583000 @default.
- W152194847 cites W2043181363 @default.
- W152194847 cites W2044499660 @default.
- W152194847 cites W2045093773 @default.
- W152194847 cites W2049081932 @default.
- W152194847 cites W2049946635 @default.
- W152194847 cites W2051828819 @default.
- W152194847 cites W2055217546 @default.
- W152194847 cites W2056451482 @default.
- W152194847 cites W2056821381 @default.
- W152194847 cites W2057064153 @default.
- W152194847 cites W2060544783 @default.
- W152194847 cites W2061055398 @default.
- W152194847 cites W2062270991 @default.
- W152194847 cites W2063031440 @default.
- W152194847 cites W2063677595 @default.
- W152194847 cites W2066330235 @default.
- W152194847 cites W2069927131 @default.
- W152194847 cites W2073905425 @default.
- W152194847 cites W2074795830 @default.
- W152194847 cites W2078430381 @default.
- W152194847 cites W2079642606 @default.
- W152194847 cites W2081859185 @default.
- W152194847 cites W2082891823 @default.
- W152194847 cites W2088620347 @default.
- W152194847 cites W2089703781 @default.
- W152194847 cites W2090401069 @default.
- W152194847 cites W2090547978 @default.
- W152194847 cites W2091099656 @default.
- W152194847 cites W2092049868 @default.
- W152194847 cites W2094499571 @default.