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• W1522011791 abstract "Particulate methane monooxygenase (pMMO) is a multisubunit metalloenzyme complex used by methanotrophic bacteria to oxidize methane in the first step of carbon assimilation and energy production. In this chapter, we detail methods to prepare metal free (apo) membrane-bound pMMO and to reconstitute apo pMMO with metal ions. We also describe protocols to clone, express, and refold metal-loaded soluble domain constructs of the pmoB subunit. These approaches were used to address fundamental questions concerning the metal content and location of the pMMO active site." @default.
• W1522011791 created "2016-06-24" @default.
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• W1522011791 date "2011-01-01" @default.
• W1522011791 modified "2023-09-26" @default.
• W1522011791 title "Metal Reconstitution of Particulate Methane Monooxygenase and Heterologous Expression of the pmoB Subunit" @default.
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• W1522011791 doi "https://doi.org/10.1016/b978-0-12-386905-0.00013-9" @default.
• W1522011791 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3361753" @default.
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