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- W1522333116 abstract "The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction ( PCR ) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA), adapter ligation with fragments of DNA and amplification of the fragments obtained in the earlier steps using combinations of selective primers and detection of polymorphism using radioisotopes, fluorescent dyes or silver staining. It is widely used in the evaluation of genetic diversity, analysis of genetic distance, DNA fingerprint, germoplasm collections analysis, genetic maps construction and markers diagnostic monitoring. In order to characterize molecularly two species of crops with agricultural interests (sugar cane and banana) were generated AFLP markers. In this study was demonstrated the ability to identify variations in the genome of crops studied. Key words: Musa , ‘Navolean’, Saccharum officinarum, sugarcane" @default.
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- W1522333116 date "2007-04-08" @default.
- W1522333116 modified "2023-10-18" @default.
- W1522333116 title "Empleo de los marcadores AFLP para la caracterización molecular de dos cultivos con interés agrícola" @default.
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