Matches in SemOpenAlex for { <https://semopenalex.org/work/W1522514867> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1522514867 endingPage "8692" @default.
- W1522514867 startingPage "8689" @default.
- W1522514867 abstract "Surface-exposed regions of membrane-bound myelin basic protein--the major extrinsic membrane protein of central nervous system myelin--have been implicated as possible antigenic sites in diseased myelin. With the goal of determining the extent and nature of these regions, we have prepared basic protein modified with 13CH3-enriched acetyl groups at 7 of its 13 lysine residues. The resulting protein was placed in a membrane environment and studied by NMR spectroscopy to determine the location and rates of molecular motion of the labeled side chains with respect to lipid bilayers of the membrane. When 13C NMR spectra were obtained of the acetylated protein bound to multilamellar vesicles prepared from dimyristoylphosphatidic acid in the gel state (T = 33 degrees C), conditions under which reduced motion in the lipid bilayer broadens methylene and methyl 13C resonances of the membrane beyond detection (i.e. greater than 75-100 Hz), line widths of membrane-bound protein were measured to be 7.8 Hz, an increase of 4 Hz versus free protein. A reduction of 25-30% in integrated intensity observed in protein acetyl resonances upon membrane interaction was shown to be attributable to a population of protein-aggregated liposomes whose resonances were similarly too broad to be observed. Thus, the epsilon-acetyllysyl probes distributed throughout the protein do not penetrate the dimyristoylphosphatidic acid bilayer, but must reside in the interstitial aqueous spaces at or between membrane surfaces. These findings suggest an overall surface accessibility of membrane-bound myelin basic protein and are therefore incompatible with a model for the protein involving membrane-embedded loops or regions of functional significance." @default.
- W1522514867 created "2016-06-24" @default.
- W1522514867 creator A5007554690 @default.
- W1522514867 creator A5041060172 @default.
- W1522514867 date "1984-07-01" @default.
- W1522514867 modified "2023-09-29" @default.
- W1522514867 title "Surface accessibility of 13C-labeled lysine residues in membrane-bound myelin basic protein." @default.
- W1522514867 cites W1482784897 @default.
- W1522514867 cites W1489952277 @default.
- W1522514867 cites W1516608985 @default.
- W1522514867 cites W1775749144 @default.
- W1522514867 cites W1969878628 @default.
- W1522514867 cites W1971704611 @default.
- W1522514867 cites W1983964199 @default.
- W1522514867 cites W1994219576 @default.
- W1522514867 cites W1997717664 @default.
- W1522514867 cites W1998221940 @default.
- W1522514867 cites W2003163418 @default.
- W1522514867 cites W2005314722 @default.
- W1522514867 cites W2015989566 @default.
- W1522514867 cites W2016686018 @default.
- W1522514867 cites W2019487464 @default.
- W1522514867 cites W2028648434 @default.
- W1522514867 cites W2031687175 @default.
- W1522514867 cites W2034885184 @default.
- W1522514867 cites W2036905183 @default.
- W1522514867 cites W2049520795 @default.
- W1522514867 cites W2050331924 @default.
- W1522514867 cites W2053370655 @default.
- W1522514867 cites W2056495435 @default.
- W1522514867 cites W2061773226 @default.
- W1522514867 cites W2062794850 @default.
- W1522514867 cites W2064109346 @default.
- W1522514867 cites W2080484385 @default.
- W1522514867 cites W2094170996 @default.
- W1522514867 cites W2094693379 @default.
- W1522514867 cites W2095363290 @default.
- W1522514867 cites W2095556254 @default.
- W1522514867 cites W2114879066 @default.
- W1522514867 cites W2209930309 @default.
- W1522514867 cites W4302083851 @default.
- W1522514867 doi "https://doi.org/10.1016/s0021-9258(17)47206-1" @default.
- W1522514867 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6204974" @default.
- W1522514867 hasPublicationYear "1984" @default.
- W1522514867 type Work @default.
- W1522514867 sameAs 1522514867 @default.
- W1522514867 citedByCount "11" @default.
- W1522514867 countsByYear W15225148672015 @default.
- W1522514867 countsByYear W15225148672021 @default.
- W1522514867 crossrefType "journal-article" @default.
- W1522514867 hasAuthorship W1522514867A5007554690 @default.
- W1522514867 hasAuthorship W1522514867A5041060172 @default.
- W1522514867 hasBestOaLocation W15225148671 @default.
- W1522514867 hasConcept C144647389 @default.
- W1522514867 hasConcept C169760540 @default.
- W1522514867 hasConcept C185592680 @default.
- W1522514867 hasConcept C2776016237 @default.
- W1522514867 hasConcept C2778609137 @default.
- W1522514867 hasConcept C2780876595 @default.
- W1522514867 hasConcept C41625074 @default.
- W1522514867 hasConcept C515207424 @default.
- W1522514867 hasConcept C529278444 @default.
- W1522514867 hasConcept C55493867 @default.
- W1522514867 hasConcept C86803240 @default.
- W1522514867 hasConceptScore W1522514867C144647389 @default.
- W1522514867 hasConceptScore W1522514867C169760540 @default.
- W1522514867 hasConceptScore W1522514867C185592680 @default.
- W1522514867 hasConceptScore W1522514867C2776016237 @default.
- W1522514867 hasConceptScore W1522514867C2778609137 @default.
- W1522514867 hasConceptScore W1522514867C2780876595 @default.
- W1522514867 hasConceptScore W1522514867C41625074 @default.
- W1522514867 hasConceptScore W1522514867C515207424 @default.
- W1522514867 hasConceptScore W1522514867C529278444 @default.
- W1522514867 hasConceptScore W1522514867C55493867 @default.
- W1522514867 hasConceptScore W1522514867C86803240 @default.
- W1522514867 hasIssue "14" @default.
- W1522514867 hasLocation W15225148671 @default.
- W1522514867 hasOpenAccess W1522514867 @default.
- W1522514867 hasPrimaryLocation W15225148671 @default.
- W1522514867 hasRelatedWork W2021352331 @default.
- W1522514867 hasRelatedWork W2041316641 @default.
- W1522514867 hasRelatedWork W2093235125 @default.
- W1522514867 hasRelatedWork W2123272770 @default.
- W1522514867 hasRelatedWork W2127354448 @default.
- W1522514867 hasRelatedWork W2154157043 @default.
- W1522514867 hasRelatedWork W2199721082 @default.
- W1522514867 hasRelatedWork W2308603078 @default.
- W1522514867 hasRelatedWork W2728207433 @default.
- W1522514867 hasRelatedWork W2122680488 @default.
- W1522514867 hasVolume "259" @default.
- W1522514867 isParatext "false" @default.
- W1522514867 isRetracted "false" @default.
- W1522514867 magId "1522514867" @default.
- W1522514867 workType "article" @default.