Matches in SemOpenAlex for { <https://semopenalex.org/work/W1522589810> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1522589810 abstract "Abstract Evidence has been presented in this investigation to show that the synthesis of aspartate kinase in Escherichia coli B is regulated by the ratio of the intracellular concentrations of the end products, methionine, threonine, and lysine. l-Lysine and l-threonine at a concentration of 5 mm repress the enzyme to the extent of 60 to 70% and 15 to 25%, respectively. On the other hand, methionine at a concentration of 5 mm stimulates the synthesis of aspartate kinase to the extent of 2- to 4-fold. The increased aspartate kinase activity observed when the bacteria is grown in the presence of methionine is not due to the presence of an activator of the enzyme in the cell-free extract. The increased enzyme activity is sensitive to the presence of chloramphenicol in the growth media, along with methionine. This suggests that the observed increased aspartate kinase activity in extracts obtained from cells grown in the presence of methionine is due to an additional synthesis of enzyme protein. It also appears that, under conditions in which protein synthesis is completely abolished by chloramphenicol, there is a methionine-dependent formation of a substance which subsequently promotes the synthesis of aspartate kinase upon removal of both chloramphenicol and methionine. From the heat stability data and from the results with respect to sensitivity toward lysine, it seems that the newly synthesized enzyme is a lysine-sensitive aspartate kinase. When added in vitro, methionine does not inhibit or stimulate aspartate kinase activity in the cell-free extract obtained from E. coli B grown either in minimal media or in the presence of methionine. When amino acids are used in combinations in the growth media, they seem to have an effect on the synthesis of aspartate kinase which depends upon the ratio of the intracellular concentration of the amino acids. Increased specific activity of aspartate kinase in the cell-free extract has been observed when the ratio of methionine to lysine and threonine concentrations increases beyond a certain value; decreased specific activity of the enzyme has been observed when this ratio goes below a certain value." @default.
- W1522589810 created "2016-06-24" @default.
- W1522589810 creator A5007834243 @default.
- W1522589810 creator A5059429469 @default.
- W1522589810 creator A5075087913 @default.
- W1522589810 date "1968-07-01" @default.
- W1522589810 modified "2023-09-29" @default.
- W1522589810 title "Regulation of Synthesis of Aspartate Kinase by Methionine, Threonine, and Lysine in Escherichia coli Strain B" @default.
- W1522589810 cites W101455241 @default.
- W1522589810 cites W1547207533 @default.
- W1522589810 cites W1583063357 @default.
- W1522589810 cites W1775749144 @default.
- W1522589810 cites W1849997836 @default.
- W1522589810 cites W1992166885 @default.
- W1522589810 cites W1992722939 @default.
- W1522589810 cites W2014638127 @default.
- W1522589810 cites W2056381947 @default.
- W1522589810 cites W2130274976 @default.
- W1522589810 cites W3164424637 @default.
- W1522589810 doi "https://doi.org/10.1016/s0021-9258(19)34189-4" @default.
- W1522589810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4872727" @default.
- W1522589810 hasPublicationYear "1968" @default.
- W1522589810 type Work @default.
- W1522589810 sameAs 1522589810 @default.
- W1522589810 citedByCount "7" @default.
- W1522589810 crossrefType "journal-article" @default.
- W1522589810 hasAuthorship W1522589810A5007834243 @default.
- W1522589810 hasAuthorship W1522589810A5059429469 @default.
- W1522589810 hasAuthorship W1522589810A5075087913 @default.
- W1522589810 hasBestOaLocation W15225898101 @default.
- W1522589810 hasConcept C104317684 @default.
- W1522589810 hasConcept C181199279 @default.
- W1522589810 hasConcept C184235292 @default.
- W1522589810 hasConcept C185592680 @default.
- W1522589810 hasConcept C2775880066 @default.
- W1522589810 hasConcept C2776016237 @default.
- W1522589810 hasConcept C2776414213 @default.
- W1522589810 hasConcept C2778473333 @default.
- W1522589810 hasConcept C2780912031 @default.
- W1522589810 hasConcept C501593827 @default.
- W1522589810 hasConcept C515207424 @default.
- W1522589810 hasConcept C547475151 @default.
- W1522589810 hasConcept C55493867 @default.
- W1522589810 hasConcept C86803240 @default.
- W1522589810 hasConcept C97029542 @default.
- W1522589810 hasConceptScore W1522589810C104317684 @default.
- W1522589810 hasConceptScore W1522589810C181199279 @default.
- W1522589810 hasConceptScore W1522589810C184235292 @default.
- W1522589810 hasConceptScore W1522589810C185592680 @default.
- W1522589810 hasConceptScore W1522589810C2775880066 @default.
- W1522589810 hasConceptScore W1522589810C2776016237 @default.
- W1522589810 hasConceptScore W1522589810C2776414213 @default.
- W1522589810 hasConceptScore W1522589810C2778473333 @default.
- W1522589810 hasConceptScore W1522589810C2780912031 @default.
- W1522589810 hasConceptScore W1522589810C501593827 @default.
- W1522589810 hasConceptScore W1522589810C515207424 @default.
- W1522589810 hasConceptScore W1522589810C547475151 @default.
- W1522589810 hasConceptScore W1522589810C55493867 @default.
- W1522589810 hasConceptScore W1522589810C86803240 @default.
- W1522589810 hasConceptScore W1522589810C97029542 @default.
- W1522589810 hasLocation W15225898101 @default.
- W1522589810 hasOpenAccess W1522589810 @default.
- W1522589810 hasPrimaryLocation W15225898101 @default.
- W1522589810 hasRelatedWork W101455241 @default.
- W1522589810 hasRelatedWork W1492167474 @default.
- W1522589810 hasRelatedWork W1514854062 @default.
- W1522589810 hasRelatedWork W1541632951 @default.
- W1522589810 hasRelatedWork W1894211050 @default.
- W1522589810 hasRelatedWork W190834390 @default.
- W1522589810 hasRelatedWork W1970891965 @default.
- W1522589810 hasRelatedWork W1974344060 @default.
- W1522589810 hasRelatedWork W1983648680 @default.
- W1522589810 hasRelatedWork W1990116991 @default.
- W1522589810 hasRelatedWork W1993543608 @default.
- W1522589810 hasRelatedWork W1995468523 @default.
- W1522589810 hasRelatedWork W2004519796 @default.
- W1522589810 hasRelatedWork W2045420941 @default.
- W1522589810 hasRelatedWork W2085576009 @default.
- W1522589810 hasRelatedWork W2096838157 @default.
- W1522589810 hasRelatedWork W2122106817 @default.
- W1522589810 hasRelatedWork W2336896751 @default.
- W1522589810 hasRelatedWork W2408881971 @default.
- W1522589810 hasRelatedWork W1510926707 @default.
- W1522589810 isParatext "false" @default.
- W1522589810 isRetracted "false" @default.
- W1522589810 magId "1522589810" @default.
- W1522589810 workType "article" @default.