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- W1522682906 abstract "For the selection of a suitable surface sterilization procedure for in-vitro establishment of seeds, 0.1 % Mercuric Chloride was used with five different exposure times (5, 10, 15, 20, and 25 min). Pods with their highest external diameter of 5 cm were used to examine the effect of pod size on in-vitro germination and pods harvested at light brown stage and, stored at ambient temperature (28 ±2 °C) for 1, 2, 3, and 4 weeks, were used to examine the effect of storage time on in-vitro germination. To study the effect of different culture conditions on in-vitro germination, seeds were cultured on hormone free Murashige & Skoog (1962), Anderson (1980), Vitis (1987), and Woody Plant Medium (1980) with (1 g/l) and without activated charcoal. To study the in-vitro growth performance of red sandalwood seedlings, germinated seeds were cultured on hormone free MS, Anderson, and Woody Plant Medium at their full strength incorporated with 1 g/l of activated charcoal. Dipping the pods in 0.1 % HgCl2 with 2-3 drops of Tween 20 for fifteen minutes was suitable for surface sterilization. Pods with 4 cm in diameter showed a significantly higher germination (90 %) ability, while time taken for germination was not affected by pod diameter. Success of seed germination was reduced with increasing storage time. Seeds cultured within one week of harvest showed the highest germination (96 %) and short germination time (6 days), while seeds cultured after 4 weeks showed relatively less germination (61 %) and longer germination time (10 days). Germination percentage was significantly higher (92%) in Anderson medium without charcoal, and low germination percentages of 62, 62 and 61 % were recorded on seeds cultured on woody plant medium (with charcoal), MS and Vitis medium (without charcoal), respectively. Seeds cultured on Anderson, Vitis and woody plant medium took almost similar time (6-8 days) for germination. Seeds cultured on Vitis medium (with charcoal) showed the longest hypocotyls length, 13.8 mm. The highest plant height of 9.9 cm and highest number of nodes per seedling (7 nodes/shoot)" @default.
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- W1522682906 date "2006-01-01" @default.
- W1522682906 modified "2023-09-22" @default.
- W1522682906 title "In vitro establishment germination and growth performance of red sandalwood (Pterocarpus santalinus L.)" @default.
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