Matches in SemOpenAlex for { <https://semopenalex.org/work/W1522761271> ?p ?o ?g. }
- W1522761271 endingPage "26617" @default.
- W1522761271 startingPage "26613" @default.
- W1522761271 abstract "Aldosterone treatment of A6 cultured renal epithelial cells methylates the apical membrane, and we examined the aldosterone-induced carboxymethylation of the apical membrane of these cells to determine the targeted polypeptides. Methionine-deprived A6 cells were incubated with aldosterone and [3H]methionine. Homogenates and apical membranes were solubilized and analyzed by SDS-polyacrylamide gel electrophoresis. Label incorporation in a 90-kDa polypeptide was more intense (4-fold) in membranes after aldosterone compared to control. For in vitro methylation, membranes were isolated, incubated with S-adenosyl-L-[methyl-3H]methionine, and analyzed for 3H-methyl uptake. Label incorporation was low in control membranes but markedly stimulated (4-fold) in membrane preparations from aldosterone-treated cells. Guanosine 5'-O-(3-thiotriphosphate) increased in vitro methylation of a 90-kDa polypeptide 5-fold in control membranes but after aldosterone, where methylation was already stimulated, little change was observed. We conclude that aldosterone induces methylation of an apical membrane 90-kDa polypeptide, possibly a subunit of the epithelial Na+ channel, in a GTP-dependent manner, and this may be one of the final steps in a cascade of reactions leading to the natriferic action of this hormone." @default.
- W1522761271 created "2016-06-24" @default.
- W1522761271 creator A5030504095 @default.
- W1522761271 creator A5053953732 @default.
- W1522761271 creator A5063369311 @default.
- W1522761271 date "1993-12-01" @default.
- W1522761271 modified "2023-10-15" @default.
- W1522761271 title "Aldosterone-induced and GTP-stimulated methylation of a 90-kDa polypeptide in the apical membrane of A6 epithelia." @default.
- W1522761271 cites W1489559813 @default.
- W1522761271 cites W1506512444 @default.
- W1522761271 cites W1508018835 @default.
- W1522761271 cites W1515287375 @default.
- W1522761271 cites W1532322015 @default.
- W1522761271 cites W1533306569 @default.
- W1522761271 cites W1533750627 @default.
- W1522761271 cites W1539027745 @default.
- W1522761271 cites W1558046708 @default.
- W1522761271 cites W1606227954 @default.
- W1522761271 cites W1926935438 @default.
- W1522761271 cites W1968123845 @default.
- W1522761271 cites W2004250443 @default.
- W1522761271 cites W2013498385 @default.
- W1522761271 cites W2023587184 @default.
- W1522761271 cites W2034801578 @default.
- W1522761271 cites W2039451581 @default.
- W1522761271 cites W2044415802 @default.
- W1522761271 cites W2057867770 @default.
- W1522761271 cites W2058045896 @default.
- W1522761271 cites W2058449239 @default.
- W1522761271 cites W2078023613 @default.
- W1522761271 cites W2079700930 @default.
- W1522761271 cites W2080941550 @default.
- W1522761271 cites W2083003235 @default.
- W1522761271 cites W2090117730 @default.
- W1522761271 cites W2100837269 @default.
- W1522761271 cites W2104529835 @default.
- W1522761271 cites W2134460161 @default.
- W1522761271 cites W2176699929 @default.
- W1522761271 cites W2180133926 @default.
- W1522761271 cites W2239181400 @default.
- W1522761271 cites W2279422544 @default.
- W1522761271 cites W2410369455 @default.
- W1522761271 cites W2411129358 @default.
- W1522761271 cites W2413111528 @default.
- W1522761271 cites W2438816601 @default.
- W1522761271 cites W51263613 @default.
- W1522761271 doi "https://doi.org/10.1016/s0021-9258(19)74356-7" @default.
- W1522761271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8253792" @default.
- W1522761271 hasPublicationYear "1993" @default.
- W1522761271 type Work @default.
- W1522761271 sameAs 1522761271 @default.
- W1522761271 citedByCount "32" @default.
- W1522761271 countsByYear W15227612712015 @default.
- W1522761271 countsByYear W15227612712016 @default.
- W1522761271 countsByYear W15227612712023 @default.
- W1522761271 crossrefType "journal-article" @default.
- W1522761271 hasAuthorship W1522761271A5030504095 @default.
- W1522761271 hasAuthorship W1522761271A5053953732 @default.
- W1522761271 hasAuthorship W1522761271A5063369311 @default.
- W1522761271 hasBestOaLocation W15227612711 @default.
- W1522761271 hasConcept C104317684 @default.
- W1522761271 hasConcept C118716 @default.
- W1522761271 hasConcept C126322002 @default.
- W1522761271 hasConcept C134018914 @default.
- W1522761271 hasConcept C181199279 @default.
- W1522761271 hasConcept C185592680 @default.
- W1522761271 hasConcept C2778525890 @default.
- W1522761271 hasConcept C3018436504 @default.
- W1522761271 hasConcept C33288867 @default.
- W1522761271 hasConcept C41625074 @default.
- W1522761271 hasConcept C55493867 @default.
- W1522761271 hasConcept C71924100 @default.
- W1522761271 hasConcept C86803240 @default.
- W1522761271 hasConcept C95444343 @default.
- W1522761271 hasConceptScore W1522761271C104317684 @default.
- W1522761271 hasConceptScore W1522761271C118716 @default.
- W1522761271 hasConceptScore W1522761271C126322002 @default.
- W1522761271 hasConceptScore W1522761271C134018914 @default.
- W1522761271 hasConceptScore W1522761271C181199279 @default.
- W1522761271 hasConceptScore W1522761271C185592680 @default.
- W1522761271 hasConceptScore W1522761271C2778525890 @default.
- W1522761271 hasConceptScore W1522761271C3018436504 @default.
- W1522761271 hasConceptScore W1522761271C33288867 @default.
- W1522761271 hasConceptScore W1522761271C41625074 @default.
- W1522761271 hasConceptScore W1522761271C55493867 @default.
- W1522761271 hasConceptScore W1522761271C71924100 @default.
- W1522761271 hasConceptScore W1522761271C86803240 @default.
- W1522761271 hasConceptScore W1522761271C95444343 @default.
- W1522761271 hasIssue "35" @default.
- W1522761271 hasLocation W15227612711 @default.
- W1522761271 hasOpenAccess W1522761271 @default.
- W1522761271 hasPrimaryLocation W15227612711 @default.
- W1522761271 hasRelatedWork W1578550702 @default.
- W1522761271 hasRelatedWork W1981040892 @default.
- W1522761271 hasRelatedWork W2094513741 @default.
- W1522761271 hasRelatedWork W2096111588 @default.
- W1522761271 hasRelatedWork W2313324861 @default.
- W1522761271 hasRelatedWork W2337581113 @default.