FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1523009458> ?p ?o ?g. }

• W1523009458 abstract "In this paper, we present a new and simple purification of the 36-kDa protein, a major substrate of both viral and growth factor-receptor associated tyrosine protein kinases, and its complex from normal and Schmidt-Ruppin strain Rous sarcoma virus-transformed chicken embryo fibroblasts that employs a DEAE-Sephacel column and introduces the calcium-dependent adsorption of 36-kDa protein. The use of EGTA step gradients differentially elutes the 36-kDa molecules from the DEAE-Sephacel column. An average total yield of the 36-kDa protein in all fractions approached 80% and represented 0.78% of the [35S] methionine-labeled cellular protein. A purity of 95-99% was obtained with a yield of 60% in the central elution fractions from normal or Rous sarcoma virus-transformed chicken embryo fibroblasts. Furthermore, 2 mM EGTA elutes poorly phosphorylated molecules while heavily phosphorylated 36-kDa protein requires 4 or 6 M EGTA; a small residual fraction is released at 8-10 mM EGTA. If the EGTA step gradients were neutralized with Ca2+ ion, elution of the 36-kDa protein is inhibited. The complex of the 36-kDa protein and the 6-10-kDa protein may not be dependent on the phosphorylation as the associated 6-10-kDa peptide is seen in all fractions containing the 36-kDa protein. Tyrosine phosphorylation of the 36-kDa protein is increased 2-3-fold following a short term incubation of whole cells with micromolar vanadate. The elution pattern (but not intensity) of the 36-kDa protein obtained from lysates of vanadate-treated cells was identical to untreated cell lysates. The additional phosphorylation appears to result from a recruitment of unphosphorylated 36-kDa protein as the position (but not intensity) of the phosphorylated tryptic peptides is unchanged. We conclude that the function of the 36-kDa protein may be calcium ion-dependent and may be influenced by the phosphorylation state of the protein." @default.
• W1523009458 created "2016-06-24" @default.
• W1523009458 creator A5052786886 @default.
• W1523009458 creator A5055149770 @default.
• W1523009458 date "1986-11-01" @default.
• W1523009458 modified "2023-09-28" @default.
• W1523009458 title "Calcium-dependent isolation of the 36-kilodalton substrate of pp60src-kinase. Fractionation of the phosphorylated and unphosphorylated species." @default.
• W1523009458 cites W144955152 @default.
• W1523009458 cites W1507444500 @default.
• W1523009458 cites W1565354947 @default.
• W1523009458 cites W1575818884 @default.
• W1523009458 cites W1579211087 @default.
• W1523009458 cites W1598104246 @default.
• W1523009458 cites W1616609814 @default.
• W1523009458 cites W1943889027 @default.
• W1523009458 cites W1970081885 @default.
• W1523009458 cites W1996900767 @default.
• W1523009458 cites W1999477520 @default.
• W1523009458 cites W2000185263 @default.
• W1523009458 cites W2011147958 @default.
• W1523009458 cites W2024504944 @default.
• W1523009458 cites W2037491928 @default.
• W1523009458 cites W2064872234 @default.
• W1523009458 cites W2069576619 @default.
• W1523009458 cites W2100837269 @default.
• W1523009458 cites W2151870592 @default.
• W1523009458 cites W2166691181 @default.
• W1523009458 cites W2988163198 @default.
• W1523009458 cites W4250278165 @default.
• W1523009458 cites W4293247451 @default.
• W1523009458 cites W84630131 @default.
• W1523009458 doi "https://doi.org/10.1016/s0021-9258(18)66896-6" @default.
• W1523009458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3021742" @default.
• W1523009458 hasPublicationYear "1986" @default.
• W1523009458 type Work @default.
• W1523009458 sameAs 1523009458 @default.
• W1523009458 citedByCount "9" @default.
• W1523009458 crossrefType "journal-article" @default.
• W1523009458 hasAuthorship W1523009458A5052786886 @default.
• W1523009458 hasAuthorship W1523009458A5055149770 @default.
• W1523009458 hasBestOaLocation W15230094581 @default.
• W1523009458 hasConcept C104317684 @default.
• W1523009458 hasConcept C11960822 @default.
• W1523009458 hasConcept C123894998 @default.
• W1523009458 hasConcept C153911025 @default.
• W1523009458 hasConcept C178790620 @default.
• W1523009458 hasConcept C181199279 @default.
• W1523009458 hasConcept C185592680 @default.
• W1523009458 hasConcept C2776165026 @default.
• W1523009458 hasConcept C2777441520 @default.
• W1523009458 hasConcept C2777658833 @default.
• W1523009458 hasConcept C29688787 @default.
• W1523009458 hasConcept C40767141 @default.
• W1523009458 hasConcept C519063684 @default.
• W1523009458 hasConcept C55493867 @default.
• W1523009458 hasConcept C86803240 @default.
• W1523009458 hasConcept C87325107 @default.
• W1523009458 hasConcept C88532467 @default.
• W1523009458 hasConcept C97029542 @default.
• W1523009458 hasConceptScore W1523009458C104317684 @default.
• W1523009458 hasConceptScore W1523009458C11960822 @default.
• W1523009458 hasConceptScore W1523009458C123894998 @default.
• W1523009458 hasConceptScore W1523009458C153911025 @default.
• W1523009458 hasConceptScore W1523009458C178790620 @default.
• W1523009458 hasConceptScore W1523009458C181199279 @default.
• W1523009458 hasConceptScore W1523009458C185592680 @default.
• W1523009458 hasConceptScore W1523009458C2776165026 @default.
• W1523009458 hasConceptScore W1523009458C2777441520 @default.
• W1523009458 hasConceptScore W1523009458C2777658833 @default.
• W1523009458 hasConceptScore W1523009458C29688787 @default.
• W1523009458 hasConceptScore W1523009458C40767141 @default.
• W1523009458 hasConceptScore W1523009458C519063684 @default.
• W1523009458 hasConceptScore W1523009458C55493867 @default.
• W1523009458 hasConceptScore W1523009458C86803240 @default.
• W1523009458 hasConceptScore W1523009458C87325107 @default.
• W1523009458 hasConceptScore W1523009458C88532467 @default.
• W1523009458 hasConceptScore W1523009458C97029542 @default.
• W1523009458 hasLocation W15230094581 @default.
• W1523009458 hasOpenAccess W1523009458 @default.
• W1523009458 hasPrimaryLocation W15230094581 @default.
• W1523009458 hasRelatedWork W144955152 @default.
• W1523009458 hasRelatedWork W1507444500 @default.
• W1523009458 hasRelatedWork W1510923466 @default.
• W1523009458 hasRelatedWork W1605429420 @default.
• W1523009458 hasRelatedWork W184226509 @default.
• W1523009458 hasRelatedWork W1969422658 @default.
• W1523009458 hasRelatedWork W1997988665 @default.
• W1523009458 hasRelatedWork W1999477520 @default.
• W1523009458 hasRelatedWork W2021131127 @default.
• W1523009458 hasRelatedWork W2037491928 @default.
• W1523009458 hasRelatedWork W2085292543 @default.
• W1523009458 hasRelatedWork W2113225489 @default.
• W1523009458 hasRelatedWork W2120654193 @default.
• W1523009458 hasRelatedWork W2124483822 @default.
• W1523009458 hasRelatedWork W2139127386 @default.
• W1523009458 hasRelatedWork W259489311 @default.
• W1523009458 hasRelatedWork W260602016 @default.
• W1523009458 hasRelatedWork W301379775 @default.
• W1523009458 hasRelatedWork W309529471 @default.
• W1523009458 hasRelatedWork W2032725955 @default.

• next