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- W152303254 abstract "In the early eighties, a series of papers were published to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallographic studies. Specific methods were developed to demonstrate endogenous zinc pools of zinc ions in synaptic and secretory vesicles; exogenous mercury, silver bound as sulphide or selenide crystals, in lysosomes; and tissue-bound gold ions resulting from medicamented aurothiocompounds. It was demonstrated that gold ions chemically bound in the lysosomes had to be reduced to metallic gold before they could be silver-amplified. This understanding made it feasible to apply autometallographic amplification for magnifying colloidal-gold particles in thick, semithin and ultrathin sections. As a result, the highly sensitive in situ colloidal gold-labeled detection of peptides, proteins and amines by immunocytochemistry (immuno gold-silver staining [IGSS]), carbohydrates by lectin histochemistry; and DNA and RNA by in situ hybridization, in situ PCR and in situ 3SR techniques were born. Here, we present an overview of the literature on IGSS techniques used for light and electron microscopic studies and also give guidelines for using IGSS based on our own experience." @default.
- W152303254 created "2016-06-24" @default.
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- W152303254 date "1996-01-01" @default.
- W152303254 modified "2023-09-27" @default.
- W152303254 title "Immunogold-Silver Staining — Autometallography: Recent Developments and Protocols" @default.
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- W152303254 doi "https://doi.org/10.1007/978-1-4612-4166-9_2" @default.
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