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• W1523048290 abstract "Abstract In this study, the X‐ray crystal structure of the complex between Escherichia coli thioredoxin reductase (EC TrxR) and its substrate thioredoxin (Trx) was used as a guide to design a Deinococcus radiodurans TrxR (DR TrxR) mutant with altered Trx specificity. Previous studies have shown that TrxRs have higher affinity for cognate Trxs (same species) than that for Trxs from different species. Computational alanine scanning mutagenesis and visual inspection of the EC TrxR–Trx interface suggested that only four residues (F81, R130, F141, and F142) account for the majority of the EC TrxR–Trx interface stability. Individual replacement of equivalent residues in DR TrxR (M84, K137, F148, and F149) with alanine resulted in drastic changes in binding affinity, confirming that the four residues account for most of TrxR–Trx interface stability. When M84 and K137 were changed to match equivalent EC TrxR residues (K137R and M84F), the DR TrxR substrate specificity was altered from its own Trx to that of EC Trx. The results suggest that a small subset of the TrxR–Trx interface residues is responsible for the majority of Trx binding affinity and species‐specific recognition." @default.
• W1523048290 created "2016-06-24" @default.
• W1523048290 creator A5027778013 @default.
• W1523048290 creator A5066697312 @default.
• W1523048290 date "2011-05-03" @default.
• W1523048290 modified "2023-10-11" @default.
• W1523048290 title "Design of <i>Deinococcus radiodurans</i> thioredoxin reductase with altered thioredoxin specificity using computational alanine mutagenesis" @default.
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• W1523048290 doi "https://doi.org/10.1002/pro.635" @default.
• W1523048290 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3104231" @default.
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