FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1523362802> ?p ?o ?g. }

• W1523362802 abstract "Vanadate is a phosphate analogue that inhibits enzymes involved in phosphate release and transfer reactions (Simons, T. J. B. (1979) Nature 281, 337-338). Since such reactions may play important roles in endocytosis, we studied the effects of vanadate on various steps in receptor-mediated endocytosis of asialoorosomucoid labeled with 125I-tyramine-cellobiose (125I-TC-AOM). The labeled degradation products formed from 125I-TC-AOM are trapped in the lysosomes and may therefore serve as lysosomal markers in subcellular fractionation studies. Vanadate reduced the amount of active surface asialoglycoprotein receptors approximately 70%, but had no effect on the rate of internalization and retroendocytosis of ligand. The amount of surface asialoglycoprotein receptors can be reduced by lowering the incubation temperature gradually from 37 to 15 degrees C (Weigel, P. H., and Oka, J. A. (1983) J. Biol. Chem. 258, 5089-5094); vanadate affected only the temperature--sensitive receptors. Vanadate inhibited degradation of 125I-TC-AOM 70-80%. Degradation was much more sensitive to vanadate than binding; half-maximal effects were seen at approximately 1 mM vanadate for binding and approximately 0.1 mM vanadate for degradation. By subcellular fractionation in sucrose and Nycodenz gradients, it was shown that vanadate completely prevented the transfer of 125I-TC-AOM from endosomes to lysosomes. Therefore, the inhibition of degradation by vanadate was indirect; in the presence of vanadate, ligand did not gain access to the lysosomes. The limited degradation in the presence of vanadate took place in a prelysosomal compartment. Vanadate did not affect cell viability and ATP content." @default.
• W1523362802 created "2016-06-24" @default.
• W1523362802 creator A5052866754 @default.
• W1523362802 creator A5065502172 @default.
• W1523362802 creator A5085173343 @default.
• W1523362802 date "1990-06-01" @default.
• W1523362802 modified "2023-09-28" @default.
• W1523362802 title "The effect of vanadate on receptor-mediated endocytosis of asialoorosomucoid in rat liver parenchymal cells." @default.
• W1523362802 cites W1490154674 @default.
• W1523362802 cites W1495205460 @default.
• W1523362802 cites W1502498409 @default.
• W1523362802 cites W1507615631 @default.
• W1523362802 cites W1522075655 @default.
• W1523362802 cites W1527390585 @default.
• W1523362802 cites W1529494662 @default.
• W1523362802 cites W1533957803 @default.
• W1523362802 cites W1537290167 @default.
• W1523362802 cites W1547729980 @default.
• W1523362802 cites W1553159886 @default.
• W1523362802 cites W1554036089 @default.
• W1523362802 cites W1562951114 @default.
• W1523362802 cites W1574593230 @default.
• W1523362802 cites W1579174150 @default.
• W1523362802 cites W1585703295 @default.
• W1523362802 cites W1599507177 @default.
• W1523362802 cites W1848295934 @default.
• W1523362802 cites W1878414703 @default.
• W1523362802 cites W1982200763 @default.
• W1523362802 cites W1994419917 @default.
• W1523362802 cites W1995634551 @default.
• W1523362802 cites W1997687891 @default.
• W1523362802 cites W2001578819 @default.
• W1523362802 cites W2003167913 @default.
• W1523362802 cites W2005669461 @default.
• W1523362802 cites W2026876894 @default.
• W1523362802 cites W2030971353 @default.
• W1523362802 cites W2037152050 @default.
• W1523362802 cites W2040116591 @default.
• W1523362802 cites W2044140136 @default.
• W1523362802 cites W2044593160 @default.
• W1523362802 cites W2047307597 @default.
• W1523362802 cites W205180606 @default.
• W1523362802 cites W2057975341 @default.
• W1523362802 cites W2060803650 @default.
• W1523362802 cites W2084520638 @default.
• W1523362802 cites W2085344344 @default.
• W1523362802 cites W2087124122 @default.
• W1523362802 cites W2153151822 @default.
• W1523362802 cites W2168385874 @default.
• W1523362802 cites W2189293602 @default.
• W1523362802 cites W2307563242 @default.
• W1523362802 cites W2315342741 @default.
• W1523362802 cites W44464786 @default.
• W1523362802 doi "https://doi.org/10.1016/s0021-9258(19)38802-7" @default.
• W1523362802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2345164" @default.
• W1523362802 hasPublicationYear "1990" @default.
• W1523362802 type Work @default.
• W1523362802 sameAs 1523362802 @default.
• W1523362802 citedByCount "26" @default.
• W1523362802 countsByYear W15233628022021 @default.
• W1523362802 crossrefType "journal-article" @default.
• W1523362802 hasAuthorship W1523362802A5052866754 @default.
• W1523362802 hasAuthorship W1523362802A5065502172 @default.
• W1523362802 hasAuthorship W1523362802A5085173343 @default.
• W1523362802 hasBestOaLocation W15233628021 @default.
• W1523362802 hasConcept C102747710 @default.
• W1523362802 hasConcept C11569505 @default.
• W1523362802 hasConcept C12554922 @default.
• W1523362802 hasConcept C170493617 @default.
• W1523362802 hasConcept C181199279 @default.
• W1523362802 hasConcept C185592680 @default.
• W1523362802 hasConcept C202751555 @default.
• W1523362802 hasConcept C27239569 @default.
• W1523362802 hasConcept C2776200302 @default.
• W1523362802 hasConcept C2781116636 @default.
• W1523362802 hasConcept C28005876 @default.
• W1523362802 hasConcept C55493867 @default.
• W1523362802 hasConcept C86803240 @default.
• W1523362802 hasConcept C95444343 @default.
• W1523362802 hasConceptScore W1523362802C102747710 @default.
• W1523362802 hasConceptScore W1523362802C11569505 @default.
• W1523362802 hasConceptScore W1523362802C12554922 @default.
• W1523362802 hasConceptScore W1523362802C170493617 @default.
• W1523362802 hasConceptScore W1523362802C181199279 @default.
• W1523362802 hasConceptScore W1523362802C185592680 @default.
• W1523362802 hasConceptScore W1523362802C202751555 @default.
• W1523362802 hasConceptScore W1523362802C27239569 @default.
• W1523362802 hasConceptScore W1523362802C2776200302 @default.
• W1523362802 hasConceptScore W1523362802C2781116636 @default.
• W1523362802 hasConceptScore W1523362802C28005876 @default.
• W1523362802 hasConceptScore W1523362802C55493867 @default.
• W1523362802 hasConceptScore W1523362802C86803240 @default.
• W1523362802 hasConceptScore W1523362802C95444343 @default.
• W1523362802 hasLocation W15233628021 @default.
• W1523362802 hasOpenAccess W1523362802 @default.
• W1523362802 hasPrimaryLocation W15233628021 @default.
• W1523362802 hasRelatedWork W1490154674 @default.
• W1523362802 hasRelatedWork W1491606924 @default.
• W1523362802 hasRelatedWork W1500095039 @default.
• W1523362802 hasRelatedWork W1515120551 @default.

• next