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- W1523432689 abstract "As part of a program to develop transgenic `Royal Gala' apple (Malus x domestica Borkh), long-term exposure to antibiotics was compared with short-term vacuum infiltration with an acidified (pH 3.0) medium and/or a range of antibiotics for their effect on shoot regeneration and on eliminating Agrobacterium tumefaciens, supervirulent strain EHA101, from apple leaf explants following co-cultivation. None of the antibiotics, carbenicillin (crb), cefotaxime (cef) or mefoxin (mef), when incorporated into regeneration medium at 100 μg ml -1 1 eliminated A. tumefaciens from explants and cef inhibited shoot regeneration. Vacuum infiltration with either crb, cef, or mef at 2000 μg ml - for 30 min did not inhibit shoot regeneration; however, none of the treatments eliminated A. tumefaciens from explants. Vacuum infiltration with either acidified medium for 1 h or cef (5000 μg ml -1 ) for 18 h reduced contamination by about 20 - 30 %, whereas combining the two treatments reduced contamination with A. tumefaciens by 87 %. Kanamycin (kan) resistant, putative transformants without A. tumefaciens were generated when explants, following a 2-d co-cultivation with strain EHA101, were vacuum infiltrated for 1 h with an acidified medium, followed by an 18 h vacuum infiltration with cef at 5000 μg ml -1 and incubated for 52 days on regeneration and elongation media with crb (100 μg ml -1 ), mef (100 μg ml -1 ), and kan (10 μg ml -1 )." @default.
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- W1523432689 date "2000-09-01" @default.
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- W1523432689 title "GENERATING APPLE TRANSFORMANTS FREE OF AGROBACTERIUM TUMEFACIENS BY VACUUM INFILTRATING EXPLANTS WITH AN ACIDIFIED MEDIUM AND WITH ANTIBIOTICS" @default.
- W1523432689 doi "https://doi.org/10.17660/actahortic.2000.530.10" @default.
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