Matches in SemOpenAlex for { <https://semopenalex.org/work/W1523608913> ?p ?o ?g. }
- W1523608913 endingPage "n/a" @default.
- W1523608913 startingPage "n/a" @default.
- W1523608913 abstract "We compared airway epithelial cell models relevant for cystic fibrosis (CF): 16HBE cells with endogenous wild-type cystic fibrosis transmembrane conductance regulator (CFTR), CFBE cells with mutated ΔF508-CFTR, corrected CFBE cells overexpressing CFTR, CFSME (CF submucosal) and Calu-3 (non-CF submucosal) cells with respect to the epithelial sodium channel (ENaC), inducible NO synthase (iNOS) and mucins (MUC) (studied by quantitative Real-Time-Polymerase Chain Reaction, qRT-PCR and Western blot), and wound healing. CFBE cells had significantly more expression of β- and γ-ENaC mRNA and of β-ENaC protein than 16HBE cells. Compared to corrected CFBE cells, CFBE cells had increased mRNA expression of all ENaC subunits and β-ENaC protein. For ENaC, the CFSME/Calu-3 mRNA ratio was very low and contradictory to the ENaC upregulation in CF cells. CFBE cells showed decreased expression of iNOS at both mRNA and protein levels compared to 16HBE cells and only at the mRNA level compared to corrected CFBE cells. CFSME cells showed expression of iNOS whereas Calu-3 cells did not. Higher expression of MUC2 and MUC5B was found in corrected CFBE cells compared to CFBE cells. Wound healing in CFBE cells was delayed compared to corrected CFBE cells, but not to 16HBE cells, and in CFSME cells compared to Calu-3 cells. Our data suggest CFSME as an inappropriate CF cell model for Calu-3 cells, and provide partial support for the theory that the differences (in ENaC, iNOS and wound healing) between these cell lines are associated to the presence of CFTR in the bronchial airway epithelial cells." @default.
- W1523608913 created "2016-06-24" @default.
- W1523608913 creator A5003327629 @default.
- W1523608913 creator A5051210574 @default.
- W1523608913 creator A5062644038 @default.
- W1523608913 creator A5085821237 @default.
- W1523608913 date "2013-12-01" @default.
- W1523608913 modified "2023-09-27" @default.
- W1523608913 title "ENaC, iNOS, mucins, and wound healing in cystic fibrosis airway epithelial and submucosal cells" @default.
- W1523608913 cites W1538162788 @default.
- W1523608913 cites W1545359264 @default.
- W1523608913 cites W1603121970 @default.
- W1523608913 cites W1770692756 @default.
- W1523608913 cites W1775439135 @default.
- W1523608913 cites W1964066906 @default.
- W1523608913 cites W1964151355 @default.
- W1523608913 cites W1967398861 @default.
- W1523608913 cites W1976607551 @default.
- W1523608913 cites W1976916554 @default.
- W1523608913 cites W1977743111 @default.
- W1523608913 cites W1980738765 @default.
- W1523608913 cites W1981772538 @default.
- W1523608913 cites W1983052547 @default.
- W1523608913 cites W1984350355 @default.
- W1523608913 cites W1985931909 @default.
- W1523608913 cites W1993993814 @default.
- W1523608913 cites W1996260537 @default.
- W1523608913 cites W1997303727 @default.
- W1523608913 cites W2000228858 @default.
- W1523608913 cites W2001709871 @default.
- W1523608913 cites W2005644246 @default.
- W1523608913 cites W2013014565 @default.
- W1523608913 cites W2019099644 @default.
- W1523608913 cites W2022941489 @default.
- W1523608913 cites W2028000801 @default.
- W1523608913 cites W2028628898 @default.
- W1523608913 cites W2030819680 @default.
- W1523608913 cites W2034226301 @default.
- W1523608913 cites W2041103703 @default.
- W1523608913 cites W2041272659 @default.
- W1523608913 cites W2041344034 @default.
- W1523608913 cites W2041500253 @default.
- W1523608913 cites W2046096275 @default.
- W1523608913 cites W2050573110 @default.
- W1523608913 cites W2055936876 @default.
- W1523608913 cites W2058499954 @default.
- W1523608913 cites W2062364311 @default.
- W1523608913 cites W2063565576 @default.
- W1523608913 cites W2069648225 @default.
- W1523608913 cites W2075017474 @default.
- W1523608913 cites W2077264898 @default.
- W1523608913 cites W2080797320 @default.
- W1523608913 cites W2085176861 @default.
- W1523608913 cites W2087499467 @default.
- W1523608913 cites W2088493680 @default.
- W1523608913 cites W2089942817 @default.
- W1523608913 cites W2090852087 @default.
- W1523608913 cites W2094438822 @default.
- W1523608913 cites W2097063987 @default.
- W1523608913 cites W2097968494 @default.
- W1523608913 cites W2098005321 @default.
- W1523608913 cites W2107277218 @default.
- W1523608913 cites W2120886526 @default.
- W1523608913 cites W2128867464 @default.
- W1523608913 cites W2140473761 @default.
- W1523608913 cites W2144142969 @default.
- W1523608913 cites W2148309013 @default.
- W1523608913 cites W2149437725 @default.
- W1523608913 cites W2151310499 @default.
- W1523608913 cites W2151902325 @default.
- W1523608913 cites W2154164444 @default.
- W1523608913 cites W2154335708 @default.
- W1523608913 cites W2154748602 @default.
- W1523608913 cites W2165915629 @default.
- W1523608913 cites W2169715286 @default.
- W1523608913 cites W2241091797 @default.
- W1523608913 cites W2281285245 @default.
- W1523608913 cites W2316694065 @default.
- W1523608913 cites W2477675629 @default.
- W1523608913 cites W3170075439 @default.
- W1523608913 doi "https://doi.org/10.1002/cbi3.10014" @default.
- W1523608913 hasPublicationYear "2013" @default.
- W1523608913 type Work @default.
- W1523608913 sameAs 1523608913 @default.
- W1523608913 citedByCount "1" @default.
- W1523608913 countsByYear W15236089132020 @default.
- W1523608913 crossrefType "journal-article" @default.
- W1523608913 hasAuthorship W1523608913A5003327629 @default.
- W1523608913 hasAuthorship W1523608913A5051210574 @default.
- W1523608913 hasAuthorship W1523608913A5062644038 @default.
- W1523608913 hasAuthorship W1523608913A5085821237 @default.
- W1523608913 hasConcept C104317684 @default.
- W1523608913 hasConcept C105580179 @default.
- W1523608913 hasConcept C126322002 @default.
- W1523608913 hasConcept C127561419 @default.
- W1523608913 hasConcept C142724271 @default.
- W1523608913 hasConcept C1491633281 @default.
- W1523608913 hasConcept C153911025 @default.