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- W1523733521 abstract "Ocimum sanctum (Holy basil), belonging to Lamiaceae family, is a herb native toIndia. It is regarded as one of the most important plant used in Ayurvedic medicine which constituteursolic acid (3-â-hydroxyurs-12-en-oic acid), a pentacyclic triterpene acid that is used in cosmeticsand also capable of inhibiting various types of cancer cells. The current study describes thedevelopment and validation of a simple and rapid reverse phase HPLC assay method for estimationof Ursolic acid in Ocimum sanctum herb powders and extract in human plasma. A HPLC systemcomprising of RP-C18 column (250 x 4.6 mm I.D., 5 micrometer (im) particle size) with solventsystem consisting of acetonitrile and water (90:10, v/v) in isocratic mode using diethyl ether-hexane(8:2, v/v) as extracting solvent system and detection at 210 nanometer (nm) using PDA detector.The proposed method was validated for its LOD, LOQ, linearity, accuracy, precision, sensitivity,system suitability and stability. The proposed method was found to be linear in the range of 2– 100 microgram (ig)/ml with correlation coefficient of 0.9999. The LOD was 1ig/ml and LOQ was5 ig/ml. The developed HPLC method was found to be precise, sensitive, accurate and reproducibleand may be used for qualitative as well as quantitative estimation of ursolic acid in Ocimumsanctum herb powders and extracts in human plasma." @default.
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- W1523733521 date "2012-01-01" @default.
- W1523733521 modified "2023-09-23" @default.
- W1523733521 title "DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FORDETERMINATION OF URSOLIC ACID IN Ocimum sanctum LINNIN HUMAN PLASMA -" @default.
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