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- W1524008445 abstract "A number of recent ion channel investigations have involved heterologous expression in Saccharomyces cerevisiae. With this system, the advantages of yeast genetic analysis are now applicable toward the study of ion channels. In addition, the microbial aspect of the system allows experiments to be designed on a scale much larger than can be accommodated by traditional electrophysiologic analysis. For instance, the ability to perform genetic screens and selections can be a powerful method for sorting through libraries of channel mutations. Similarly, screening a cDNA library for novel ion channels can be as straightforward as performing a yeast transformation and plating for growth on the appropriate test medium. The ability to generate specific host cell genotypes and the availability of the entire genome sequence are also important components of the system. The analysis of heterologous ion channels expressed in S. cerevisiae can produce information from a new perspective: simple growth phenotypes of S. cerevisiae are used to characterize channel function. Although such output is less quantitative than standard electrophysiologic analysis, it can nevertheless be compelling. Indeed, for some channel characteristics the yeast system can provide greater sensitivity than conventional electrophysiology. This chapter describes the yeast microbial genetic system for analysis of heterologous ion channels. Although ion channels expressed in S. cerevisiae cells have been analyzed by conventional electrophysiologic protocols or by microphysiometry, these techniques will not be extensively discussed. Instead, the emphasis is on using genetic techniques to alter the function of K+ channels and the reliance on growth rates to measure the output of the altered channels." @default.
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- W1524008445 date "1998-01-01" @default.
- W1524008445 modified "2023-09-23" @default.
- W1524008445 title "[6] Studying ion channels using yeast genetics" @default.
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- W1524008445 doi "https://doi.org/10.1016/s0076-6879(98)93009-9" @default.
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