FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1524778875> ?p ?o ?g. }

• W1524778875 endingPage "19903" @default.
• W1524778875 startingPage "19897" @default.
• W1524778875 abstract "Prostacyclin synthase catalyzes the conversion of prostaglandin H2 to prostacyclin, which is a powerful vasodilator and the most potent natural occurring inhibitor of platelet aggregation. In the present study, we determined the amino acid sequence of bovine prostacyclin synthase by combined protein chemical and molecular cloning techniques. The enzyme was purified and characterized from bovine aorta microsomes, and the partial amino acid sequences were determined with the native enzyme and endoproteinase Lys-C-cleaved peptides. Using primers synthesized according to the amino acid sequences, cDNA coding for prostacyclin synthase was amplified by polymerase chain reaction with bovine endothelial cell poly(A)+ RNA and cloned into pBluescript II. Nucleotide sequence analyses of the cloned cDNA inserts revealed that cDNA for this enzyme contained a 1500-base pair open reading frame coding for a 500-amino acid polypeptide with a M(r) of 56,628. COS-7 cells transfected with an expression plasmid harboring this cDNA clone expressed prostacyclin synthase activity. The primary structure of the enzyme showed structural characteristics of cytochrome P450 and exhibited a 32% identity to that of human cholesterol 7 alpha-hydroxylase. However, the identity between the amino acid sequences of bovine prostacyclin synthase and human thromboxane synthase was only 16%, and no P450 showed an identity higher than 40%, suggesting that prostacyclin synthase represents a new family in the P450 superfamily. RNA blot analysis indicated that the mRNA for prostacyclin synthase from bovine endothelial cells showed a size of approximately 2.7 kilobases and that the mRNA level increased about 3-fold by treatment of tumor necrosis factor-alpha." @default.
• W1524778875 created "2016-06-24" @default.
• W1524778875 creator A5008260162 @default.
• W1524778875 creator A5009724731 @default.
• W1524778875 creator A5014507709 @default.
• W1524778875 creator A5041718533 @default.
• W1524778875 creator A5047414438 @default.
• W1524778875 creator A5068069545 @default.
• W1524778875 creator A5072650136 @default.
• W1524778875 creator A5072653607 @default.
• W1524778875 date "1994-08-01" @default.
• W1524778875 modified "2023-10-05" @default.
• W1524778875 title "Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells." @default.
• W1524778875 cites W1214365975 @default.
• W1524778875 cites W1514194392 @default.
• W1524778875 cites W1529367350 @default.
• W1524778875 cites W1544157645 @default.
• W1524778875 cites W1585560453 @default.
• W1524778875 cites W1585808802 @default.
• W1524778875 cites W1641540149 @default.
• W1524778875 cites W1745529008 @default.
• W1524778875 cites W1775749144 @default.
• W1524778875 cites W1966843231 @default.
• W1524778875 cites W1980581864 @default.
• W1524778875 cites W1985240141 @default.
• W1524778875 cites W1996831533 @default.
• W1524778875 cites W1999772218 @default.
• W1524778875 cites W2004114416 @default.
• W1524778875 cites W2007321993 @default.
• W1524778875 cites W2009105413 @default.
• W1524778875 cites W2011611199 @default.
• W1524778875 cites W2014669590 @default.
• W1524778875 cites W2027894523 @default.
• W1524778875 cites W2038472226 @default.
• W1524778875 cites W2051107933 @default.
• W1524778875 cites W2051897188 @default.
• W1524778875 cites W2056802836 @default.
• W1524778875 cites W2057230687 @default.
• W1524778875 cites W2089749563 @default.
• W1524778875 cites W2090796233 @default.
• W1524778875 cites W2100837269 @default.
• W1524778875 cites W2134785329 @default.
• W1524778875 cites W2150946709 @default.
• W1524778875 cites W2165191737 @default.
• W1524778875 cites W2263048471 @default.
• W1524778875 cites W2280821353 @default.
• W1524778875 cites W4294216491 @default.
• W1524778875 cites W962067443 @default.
• W1524778875 doi "https://doi.org/10.1016/s0021-9258(17)32104-x" @default.
• W1524778875 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8051072" @default.
• W1524778875 hasPublicationYear "1994" @default.
• W1524778875 type Work @default.
• W1524778875 sameAs 1524778875 @default.
• W1524778875 citedByCount "120" @default.
• W1524778875 countsByYear W15247788752012 @default.
• W1524778875 countsByYear W15247788752013 @default.
• W1524778875 countsByYear W15247788752014 @default.
• W1524778875 countsByYear W15247788752015 @default.
• W1524778875 countsByYear W15247788752017 @default.
• W1524778875 countsByYear W15247788752018 @default.
• W1524778875 countsByYear W15247788752019 @default.
• W1524778875 countsByYear W15247788752020 @default.
• W1524778875 countsByYear W15247788752021 @default.
• W1524778875 countsByYear W15247788752022 @default.
• W1524778875 countsByYear W15247788752023 @default.
• W1524778875 crossrefType "journal-article" @default.
• W1524778875 hasAuthorship W1524778875A5008260162 @default.
• W1524778875 hasAuthorship W1524778875A5009724731 @default.
• W1524778875 hasAuthorship W1524778875A5014507709 @default.
• W1524778875 hasAuthorship W1524778875A5041718533 @default.
• W1524778875 hasAuthorship W1524778875A5047414438 @default.
• W1524778875 hasAuthorship W1524778875A5068069545 @default.
• W1524778875 hasAuthorship W1524778875A5072650136 @default.
• W1524778875 hasAuthorship W1524778875A5072653607 @default.
• W1524778875 hasBestOaLocation W15247788751 @default.
• W1524778875 hasConcept C104317684 @default.
• W1524778875 hasConcept C121050878 @default.
• W1524778875 hasConcept C153911025 @default.
• W1524778875 hasConcept C167625842 @default.
• W1524778875 hasConcept C185592680 @default.
• W1524778875 hasConcept C19924922 @default.
• W1524778875 hasConcept C199360897 @default.
• W1524778875 hasConcept C2775941552 @default.
• W1524778875 hasConcept C2781128415 @default.
• W1524778875 hasConcept C41008148 @default.
• W1524778875 hasConcept C55493867 @default.
• W1524778875 hasConcept C86803240 @default.
• W1524778875 hasConcept C89423630 @default.
• W1524778875 hasConceptScore W1524778875C104317684 @default.
• W1524778875 hasConceptScore W1524778875C121050878 @default.
• W1524778875 hasConceptScore W1524778875C153911025 @default.
• W1524778875 hasConceptScore W1524778875C167625842 @default.
• W1524778875 hasConceptScore W1524778875C185592680 @default.
• W1524778875 hasConceptScore W1524778875C19924922 @default.
• W1524778875 hasConceptScore W1524778875C199360897 @default.
• W1524778875 hasConceptScore W1524778875C2775941552 @default.
• W1524778875 hasConceptScore W1524778875C2781128415 @default.
• W1524778875 hasConceptScore W1524778875C41008148 @default.

• next