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- W1524983122 abstract "Despite the success of conventional Sangersequencing, significant regions of many genomesstill present major obstacles to sequencing. Here wepropose a novel approach with the potential to alleviatea wide range of sequencing difficulties. Thetechnique involves extracting target DNA sequencefrom variants generated by introduction of randommutations. The introduction of mutations does notdestroy original sequence information, but distributesit amongst multiple variants. Some of thesevariants lack problematic features of the target andare more amenable to conventional sequencing.The technique has been successfully demonstratedwith mutation levels up to an average 18% base substitutionand has been used to read previouslyintractable poly(A), AT-rich and GC-rich motifs." @default.
- W1524983122 created "2016-06-24" @default.
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- W1524983122 date "2004-01-01" @default.
- W1524983122 modified "2023-09-26" @default.
- W1524983122 title "Unlocking hidden genomic sequence" @default.
- W1524983122 hasPublicationYear "2004" @default.
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