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• W1525023896 abstract "2-Bromoethanesulfonate (BES) inhibition of methanogenesis from methanol by resting-cell suspensions or cell extracts of Methanosarcina was reversed by coenzyme M. BES inhibition of methylcoenzyme M methylreductase activity in cell-free extracts was reversed by methylcoenzyme M but not by coenzyme M. Methanol/coenzyme M methyltransferase activity was not inhibited by 10 microM BES. Inhibition of methylreductase by BES and 3-bromopropionate was competitive with methylcoenzyme M, but inhibition by 2-bromoethanol exhibited mixed kinetics. The Ki values for the inhibitors in cell-free extracts were similar to the concentrations which inhibited intact cells. BES-resistant mutants of strain 227 were apparently permeability mutants because in vitro assays showed that mutant and parent strain methylreductases were equally sensitive to BES." @default.
• W1525023896 created "2016-06-24" @default.
• W1525023896 creator A5076585216 @default.
• W1525023896 date "1983-11-01" @default.
• W1525023896 modified "2023-10-17" @default.
• W1525023896 title "Reversal of 2-bromoethanesulfonate inhibition of methanogenesis in Methanosarcina sp" @default.
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• W1525023896 doi "https://doi.org/10.1128/jb.156.2.516-523.1983" @default.
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