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• W1525121299 abstract "Astrocytes, a subtype of glial cell, have recently been shown to exhibit Ca2+ elevations in response to neurotransmitters. A Ca2+ elevation can propagate to adjacent astrocytes as a Ca2+ wave, which allows an astrocyte to communicate with its neighbors. Additionally, glutamate can be released from astrocytes via a Ca2+-dependent mechanism, thus modulating neuronal activity and synaptic transmission. In this dissertation, the author investigated the roles of another endogenous signal, nitric oxide (NO), in astrocyte-neuron signaling. First the author tested if NO is generated during astrocytic Ca2+ signaling by imaging NO in purified murine cortical astrocyte cultures. Physiological concentrations of a natural messenger, ATP, caused a Ca2+-dependent NO production. To test the roles of NO in astrocytic Ca2+ signaling, the author applied NO to astrocyte cultures via addition of a NO donor, S-nitrosol-N-acetylpenicillamine (SNAP). NO induced an influx of external Ca2+, possibly through store-operated Ca2+ channels. The NO-induced Ca2+ signaling is cGMP-independent since 8-Br-cGMP, an agonistic analog of cGMP, did not induce a detectable Ca2+ change. The consequence of this NO-induced Ca2+ influx was assessed by simultaneously monitoring of cytosolic and internal store Ca2+ using fluorescent Ca2+ indicators x-rhod-1 and mag-fluo-4. Blockage of NO signaling with the NO scavenger PTIO significantly reduced the refilling percentage of internal stores following ATP-induced Ca2+ release, suggesting that NO modulates internal store refilling. Furthermore, locally photo-release of NO to a single astrocyte led to a Ca2+ elevation in the stimulated astrocyte and a subsequent Ca2+ wave to neighbors. Finally, the author tested the role of NO inglutamate-mediated astrocyte-neuron signaling by recording the astrocyte-evoked glutamate-dependent neuronal slow inward current (SIC). Although NO is not required for the SIC,PTIO reduced SIC amplitude, suggesting that NO modulates glutamate release from astrocytes or glutamate receptor sensitivity of neurons." @default.
• W1525121299 created "2016-06-24" @default.
• W1525121299 creator A5041895206 @default.
• W1525121299 date "2002-01-01" @default.
• W1525121299 modified "2023-09-27" @default.
• W1525121299 title "Nitric Oxide in Astrocyte-Neuron Signaling" @default.
• W1525121299 cites W109894262 @default.
• W1525121299 cites W1269597682 @default.
• W1525121299 cites W1424754343 @default.
• W1525121299 cites W1492951734 @default.
• W1525121299 cites W1507841416 @default.
• W1525121299 cites W1511098597 @default.
• W1525121299 cites W1535641516 @default.
• W1525121299 cites W1543601456 @default.
• W1525121299 cites W1563544244 @default.
• W1525121299 cites W1568328657 @default.
• W1525121299 cites W1572171312 @default.
• W1525121299 cites W1586815960 @default.
• W1525121299 cites W1599277155 @default.
• W1525121299 cites W1600549717 @default.
• W1525121299 cites W1601936385 @default.
• W1525121299 cites W1629086159 @default.
• W1525121299 cites W1642980781 @default.
• W1525121299 cites W1845580528 @default.
• W1525121299 cites W1853966560 @default.
• W1525121299 cites W1864765563 @default.
• W1525121299 cites W1888295889 @default.
• W1525121299 cites W1902112862 @default.
• W1525121299 cites W1936866440 @default.
• W1525121299 cites W1941172229 @default.
• W1525121299 cites W1954747488 @default.
• W1525121299 cites W1957992664 @default.
• W1525121299 cites W1963924076 @default.
• W1525121299 cites W1965529878 @default.
• W1525121299 cites W1967453616 @default.
• W1525121299 cites W1969350873 @default.
• W1525121299 cites W1969403218 @default.
• W1525121299 cites W1969530182 @default.
• W1525121299 cites W1971274218 @default.
• W1525121299 cites W1971473766 @default.
• W1525121299 cites W1973839541 @default.
• W1525121299 cites W1974601722 @default.
• W1525121299 cites W1975333295 @default.
• W1525121299 cites W1975421593 @default.
• W1525121299 cites W1975693073 @default.
• W1525121299 cites W1975700762 @default.
• W1525121299 cites W1976142920 @default.
• W1525121299 cites W1976251878 @default.
• W1525121299 cites W1979521526 @default.
• W1525121299 cites W1980286364 @default.
• W1525121299 cites W1981837661 @default.
• W1525121299 cites W1983944086 @default.
• W1525121299 cites W1985799920 @default.
• W1525121299 cites W1985956285 @default.
• W1525121299 cites W1986271944 @default.
• W1525121299 cites W1987885030 @default.
• W1525121299 cites W1987923616 @default.
• W1525121299 cites W1988333213 @default.
• W1525121299 cites W1991692667 @default.
• W1525121299 cites W1992184689 @default.
• W1525121299 cites W1993629125 @default.
• W1525121299 cites W1996603253 @default.
• W1525121299 cites W1998901137 @default.
• W1525121299 cites W199952077 @default.
• W1525121299 cites W2000075945 @default.
• W1525121299 cites W2000886554 @default.
• W1525121299 cites W2002947972 @default.
• W1525121299 cites W2003683523 @default.
• W1525121299 cites W2005432117 @default.
• W1525121299 cites W2005591501 @default.
• W1525121299 cites W2006662265 @default.
• W1525121299 cites W2007219916 @default.
• W1525121299 cites W2007737411 @default.
• W1525121299 cites W2007910408 @default.
• W1525121299 cites W2010055809 @default.
• W1525121299 cites W2010158663 @default.
• W1525121299 cites W2010795846 @default.
• W1525121299 cites W2011564032 @default.
• W1525121299 cites W2012302730 @default.
• W1525121299 cites W2013759078 @default.
• W1525121299 cites W2015173171 @default.
• W1525121299 cites W2016204376 @default.
• W1525121299 cites W2016548049 @default.
• W1525121299 cites W2016633207 @default.
• W1525121299 cites W2017523469 @default.
• W1525121299 cites W2017869601 @default.
• W1525121299 cites W2018101787 @default.
• W1525121299 cites W2019070413 @default.
• W1525121299 cites W2019738475 @default.
• W1525121299 cites W2020669038 @default.
• W1525121299 cites W2022187477 @default.
• W1525121299 cites W2022867171 @default.
• W1525121299 cites W2023170094 @default.
• W1525121299 cites W2023883316 @default.
• W1525121299 cites W2025097191 @default.
• W1525121299 cites W2026697337 @default.
• W1525121299 cites W2027659564 @default.
• W1525121299 cites W2030033383 @default.
• W1525121299 cites W2031611126 @default.
• W1525121299 cites W2032895185 @default.

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