Matches in SemOpenAlex for { <https://semopenalex.org/work/W1525221108> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1525221108 endingPage "300" @default.
- W1525221108 startingPage "295" @default.
- W1525221108 abstract "Muteins, i.e. proteins altered by mutation of their genes, of interleukin 2 (I12) were generated by oligonucleotide-directed mutagenesis in vitro. All acidic and basic residues conserved between man and mouse were exchanged as well as four lipophilic residues contained within four hydrophobic segments of the protein. The muteins were produced in Escherichia coli and submitted to a renaturation and purification protocol, before bioactivity and receptor binding of each of them was determined. All muteins besides two (K44/T125 and Q110/T125) could be renatured and purified. One mutein (K94/T125) exhibited a more than tenfold-improved renaturation yield. One amino exchange (Asp-20 to Asn) resulted in an about 20-fold reduction in proliferative activity and high-affinity receptor binding. The binding to the low-affinity I12-binding protein (Tac antigen) was unimpaired. A second exchange (Arg-38 to Gln) had no effect on proliferative activity. The binding to both the high- and the low-affinity receptor, however, was reduced about 20-fold. Preliminary trials on the stability of these muteins by guanidinium hydrochloride denaturation studies detected no differences between wild-type interleukin 2 and muteins. It is suggested that Asp-20 forms part of the binding site for the large receptor subunit whereas Arg-38 is involved in the contact site to the small subunit." @default.
- W1525221108 created "2016-06-24" @default.
- W1525221108 creator A5025036907 @default.
- W1525221108 creator A5052740312 @default.
- W1525221108 creator A5076721870 @default.
- W1525221108 date "1989-03-01" @default.
- W1525221108 modified "2023-10-14" @default.
- W1525221108 title "Mutant proteins of human interleukin 2. Renaturation yield, proliferative activity and receptor binding" @default.
- W1525221108 cites W1506157243 @default.
- W1525221108 cites W1508418323 @default.
- W1525221108 cites W1565589271 @default.
- W1525221108 cites W1582531628 @default.
- W1525221108 cites W1775749144 @default.
- W1525221108 cites W1970162149 @default.
- W1525221108 cites W1978134609 @default.
- W1525221108 cites W1986888564 @default.
- W1525221108 cites W1993051112 @default.
- W1525221108 cites W2011060311 @default.
- W1525221108 cites W2017533728 @default.
- W1525221108 cites W2022776676 @default.
- W1525221108 cites W2051636382 @default.
- W1525221108 cites W2051879599 @default.
- W1525221108 cites W2056666064 @default.
- W1525221108 cites W2062482701 @default.
- W1525221108 cites W2090008343 @default.
- W1525221108 cites W2090737357 @default.
- W1525221108 cites W2090741624 @default.
- W1525221108 cites W2098552669 @default.
- W1525221108 cites W2100837269 @default.
- W1525221108 cites W2116076986 @default.
- W1525221108 cites W2129228470 @default.
- W1525221108 cites W2138151549 @default.
- W1525221108 cites W2141645976 @default.
- W1525221108 cites W2142301692 @default.
- W1525221108 cites W215434846 @default.
- W1525221108 cites W2157182807 @default.
- W1525221108 cites W2240211010 @default.
- W1525221108 cites W939009159 @default.
- W1525221108 doi "https://doi.org/10.1111/j.1432-1033.1989.tb14647.x" @default.
- W1525221108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2647490" @default.
- W1525221108 hasPublicationYear "1989" @default.
- W1525221108 type Work @default.
- W1525221108 sameAs 1525221108 @default.
- W1525221108 citedByCount "59" @default.
- W1525221108 countsByYear W15252211082016 @default.
- W1525221108 countsByYear W15252211082019 @default.
- W1525221108 crossrefType "journal-article" @default.
- W1525221108 hasAuthorship W1525221108A5025036907 @default.
- W1525221108 hasAuthorship W1525221108A5052740312 @default.
- W1525221108 hasAuthorship W1525221108A5076721870 @default.
- W1525221108 hasBestOaLocation W15252211081 @default.
- W1525221108 hasConcept C103408237 @default.
- W1525221108 hasConcept C104292427 @default.
- W1525221108 hasConcept C104317684 @default.
- W1525221108 hasConcept C13965031 @default.
- W1525221108 hasConcept C143065580 @default.
- W1525221108 hasConcept C153911025 @default.
- W1525221108 hasConcept C16318435 @default.
- W1525221108 hasConcept C170493617 @default.
- W1525221108 hasConcept C185592680 @default.
- W1525221108 hasConcept C2776923230 @default.
- W1525221108 hasConcept C547475151 @default.
- W1525221108 hasConcept C55493867 @default.
- W1525221108 hasConcept C86803240 @default.
- W1525221108 hasConceptScore W1525221108C103408237 @default.
- W1525221108 hasConceptScore W1525221108C104292427 @default.
- W1525221108 hasConceptScore W1525221108C104317684 @default.
- W1525221108 hasConceptScore W1525221108C13965031 @default.
- W1525221108 hasConceptScore W1525221108C143065580 @default.
- W1525221108 hasConceptScore W1525221108C153911025 @default.
- W1525221108 hasConceptScore W1525221108C16318435 @default.
- W1525221108 hasConceptScore W1525221108C170493617 @default.
- W1525221108 hasConceptScore W1525221108C185592680 @default.
- W1525221108 hasConceptScore W1525221108C2776923230 @default.
- W1525221108 hasConceptScore W1525221108C547475151 @default.
- W1525221108 hasConceptScore W1525221108C55493867 @default.
- W1525221108 hasConceptScore W1525221108C86803240 @default.
- W1525221108 hasIssue "2" @default.
- W1525221108 hasLocation W15252211081 @default.
- W1525221108 hasLocation W15252211082 @default.
- W1525221108 hasLocation W15252211083 @default.
- W1525221108 hasOpenAccess W1525221108 @default.
- W1525221108 hasPrimaryLocation W15252211081 @default.
- W1525221108 hasRelatedWork W1942501778 @default.
- W1525221108 hasRelatedWork W1985345599 @default.
- W1525221108 hasRelatedWork W2019576136 @default.
- W1525221108 hasRelatedWork W2022242968 @default.
- W1525221108 hasRelatedWork W2035318838 @default.
- W1525221108 hasRelatedWork W2040122145 @default.
- W1525221108 hasRelatedWork W2078718478 @default.
- W1525221108 hasRelatedWork W2147494701 @default.
- W1525221108 hasRelatedWork W2327483790 @default.
- W1525221108 hasRelatedWork W2471767838 @default.
- W1525221108 hasVolume "180" @default.
- W1525221108 isParatext "false" @default.
- W1525221108 isRetracted "false" @default.
- W1525221108 magId "1525221108" @default.
- W1525221108 workType "article" @default.