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- W1525310040 abstract "Abstract Chromatin fragments containing bovine 1.715 g ml-1 satellite DNA (1.715 satellite chromatin) were purified in order to test for differences between satellite chromatin and unfractionated chromatin. The final purification procedure involved digestion of isolated nuclei with Eco RI restriction endonuclease, solubilization of a fraction of the chromatin by lysis of the nuclei at low ionic strength, and chromatography of the soluble chromatin fragments on malachite green DNA affinity resin. Digestion of chromatin within steer kidney and thymus nuclei by Eco RI reached limits at which 22% and 37%, respectively, of the potential sites for the enzyme in the 1.715 satellite chromatin were cleaved. A test of the 1.715 g ml-1 satellite DNA extracted from digested thymus nuclei showed it was not significantly nicked at Eco RI sites. Fractionation of soluble chromatin fragments produced by Eco RI digestion on the basis of size using either sucrose gradient centrifugation or chromatin gel electrophoresis yielded 1.715 satellite chromatin of 60-80% purity. However, chromatography of the soluble chromatin fragments on columns of malachite green resin gave 1.715 satellite chromatin of higher purity and in greater amounts than could be obtained with the sucrose gradients or chromatin gels. Using malachite green resin, hundreds of micrograms of 1;715 satellite chromatin could be obtained with a purity that was usually at least 95% as determined by melting of the extracted DNA." @default.
- W1525310040 created "2016-06-24" @default.
- W1525310040 creator A5023208717 @default.
- W1525310040 creator A5041114832 @default.
- W1525310040 date "1982-10-01" @default.
- W1525310040 modified "2023-09-28" @default.
- W1525310040 title "Chromatin fragments containing bovine 1.715 g ml-1 satellite DNA. Purification by chromatography on malachite green DNA affinity resin." @default.
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- W1525310040 cites W1433461690 @default.
- W1525310040 cites W1485070292 @default.
- W1525310040 cites W1491269109 @default.
- W1525310040 cites W1529512777 @default.
- W1525310040 cites W1549075386 @default.
- W1525310040 cites W1553226807 @default.
- W1525310040 cites W1556041268 @default.
- W1525310040 cites W1568089172 @default.
- W1525310040 cites W1575875619 @default.
- W1525310040 cites W1600258676 @default.
- W1525310040 cites W1601806328 @default.
- W1525310040 cites W1603035059 @default.
- W1525310040 cites W1775749144 @default.
- W1525310040 cites W1913193070 @default.
- W1525310040 cites W1963602582 @default.
- W1525310040 cites W1964159868 @default.
- W1525310040 cites W1964534575 @default.
- W1525310040 cites W1966611122 @default.
- W1525310040 cites W1967446284 @default.
- W1525310040 cites W1968896207 @default.
- W1525310040 cites W1974292158 @default.
- W1525310040 cites W1975350106 @default.
- W1525310040 cites W1978927650 @default.
- W1525310040 cites W1979197786 @default.
- W1525310040 cites W1984695511 @default.
- W1525310040 cites W1990083709 @default.
- W1525310040 cites W1994820848 @default.
- W1525310040 cites W1996407941 @default.
- W1525310040 cites W2001567674 @default.
- W1525310040 cites W2005063559 @default.
- W1525310040 cites W2012500257 @default.
- W1525310040 cites W2018626566 @default.
- W1525310040 cites W2021310901 @default.
- W1525310040 cites W2024565658 @default.
- W1525310040 cites W2027393724 @default.
- W1525310040 cites W2029001616 @default.
- W1525310040 cites W2030146791 @default.
- W1525310040 cites W2030330199 @default.
- W1525310040 cites W2031572941 @default.
- W1525310040 cites W2034565654 @default.
- W1525310040 cites W2039232418 @default.
- W1525310040 cites W2043120352 @default.
- W1525310040 cites W2043264912 @default.
- W1525310040 cites W2044024506 @default.
- W1525310040 cites W2044569955 @default.
- W1525310040 cites W2045807153 @default.
- W1525310040 cites W2048374199 @default.
- W1525310040 cites W2049778420 @default.
- W1525310040 cites W2051035237 @default.
- W1525310040 cites W2056862042 @default.
- W1525310040 cites W2057092211 @default.
- W1525310040 cites W2059352301 @default.
- W1525310040 cites W2062924645 @default.
- W1525310040 cites W2064281248 @default.
- W1525310040 cites W2064480857 @default.
- W1525310040 cites W2067752183 @default.
- W1525310040 cites W2069937906 @default.
- W1525310040 cites W2070892061 @default.
- W1525310040 cites W2077365788 @default.
- W1525310040 cites W2080188266 @default.
- W1525310040 cites W2082532569 @default.
- W1525310040 cites W2084324725 @default.
- W1525310040 cites W2087070628 @default.
- W1525310040 cites W2089158354 @default.
- W1525310040 cites W2090921810 @default.
- W1525310040 cites W2091270822 @default.
- W1525310040 cites W2091752191 @default.
- W1525310040 cites W2094973062 @default.
- W1525310040 cites W209659648 @default.
- W1525310040 cites W2099114692 @default.
- W1525310040 cites W2100837269 @default.
- W1525310040 cites W2110375027 @default.
- W1525310040 cites W2143796868 @default.
- W1525310040 cites W2151764335 @default.
- W1525310040 cites W2163991860 @default.
- W1525310040 cites W2179903983 @default.
- W1525310040 cites W2215944031 @default.
- W1525310040 cites W2401504911 @default.
- W1525310040 cites W2994590824 @default.
- W1525310040 cites W3206570346 @default.
- W1525310040 cites W4242845182 @default.
- W1525310040 cites W951961178 @default.
- W1525310040 doi "https://doi.org/10.1016/s0021-9258(18)33831-6" @default.
- W1525310040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6288699" @default.
- W1525310040 hasPublicationYear "1982" @default.
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