FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1525517607> ?p ?o ?g. }

• W1525517607 endingPage "26109" @default.
• W1525517607 startingPage "26104" @default.
• W1525517607 abstract "Protein C activation is catalyzed on endothelium by a complex between thrombin and thrombomodulin. Ca2+ stimulates protein C activation in the presence, and inhibits in the absence, of thrombomodulin. Protein C has Asp residues at the P3 and P3' positions relative to the scissile bond at Arg169-Leu. To determine the contribution of these residues to the Ca2+ effect on activation, we have expressed human 4-carboxyglutamic acid (Gla)-domainless protein C and 3 mutants with Asp-->Gly substitutions at P3, P3', and both positions. Ca2+ interaction with the protein C derivatives was monitored by changes in intrinsic fluorescence, and the Ca2+ dependence of activation by thrombin and a complex of thrombin-thrombomodulin with a soluble thrombomodulin derivative (the fourth through sixth epidermal growth factor domains). The affinity for Ca2+ of the mutants was reduced 3-6-fold, which was reflected by a comparable change in the Ca2+ concentration required for the half-maximal rate of activation by the thrombin-thrombomodulin complex. However, Ca2+ no longer effectively inhibited activation of the mutants by thrombin alone. We conclude that 1) the Asp residues play a specific role in the Ca(2+)-dependent inhibition of protein C activation by thrombin; 2) these mutations alter the affinity of Ca2+ for the high affinity binding site; and 3) the Asp residues in the P3 and P3' sites do not contribute in a positive fashion to rapid activation by the thrombin-thrombomodulin complex." @default.
• W1525517607 created "2016-06-24" @default.
• W1525517607 creator A5019234325 @default.
• W1525517607 creator A5056607779 @default.
• W1525517607 date "1992-12-01" @default.
• W1525517607 modified "2023-10-06" @default.
• W1525517607 title "The function of calcium in protein C activation by thrombin and the thrombin-thrombomodulin complex can be distinguished by mutational analysis of protein C derivatives." @default.
• W1525517607 cites W107781538 @default.
• W1525517607 cites W123355458 @default.
• W1525517607 cites W1493544534 @default.
• W1525517607 cites W1500832609 @default.
• W1525517607 cites W1518197977 @default.
• W1525517607 cites W1525549376 @default.
• W1525517607 cites W1526553788 @default.
• W1525517607 cites W1528450945 @default.
• W1525517607 cites W1531765158 @default.
• W1525517607 cites W1536726659 @default.
• W1525517607 cites W1549141958 @default.
• W1525517607 cites W1552197098 @default.
• W1525517607 cites W1578821085 @default.
• W1525517607 cites W1585926352 @default.
• W1525517607 cites W1589621417 @default.
• W1525517607 cites W1595041052 @default.
• W1525517607 cites W1597632233 @default.
• W1525517607 cites W1865475665 @default.
• W1525517607 cites W1965372133 @default.
• W1525517607 cites W1965873813 @default.
• W1525517607 cites W1981765089 @default.
• W1525517607 cites W1985228189 @default.
• W1525517607 cites W2002208036 @default.
• W1525517607 cites W2018235565 @default.
• W1525517607 cites W2021157376 @default.
• W1525517607 cites W2040089477 @default.
• W1525517607 cites W2068496108 @default.
• W1525517607 cites W2069926611 @default.
• W1525517607 cites W2097803943 @default.
• W1525517607 cites W2100837269 @default.
• W1525517607 cites W2138270253 @default.
• W1525517607 cites W2145607527 @default.
• W1525517607 cites W2218435201 @default.
• W1525517607 cites W2417964224 @default.
• W1525517607 cites W4247666569 @default.
• W1525517607 cites W87735197 @default.
• W1525517607 doi "https://doi.org/10.1016/s0021-9258(18)35723-5" @default.
• W1525517607 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1334492" @default.
• W1525517607 hasPublicationYear "1992" @default.
• W1525517607 type Work @default.
• W1525517607 sameAs 1525517607 @default.
• W1525517607 citedByCount "109" @default.
• W1525517607 countsByYear W15255176072012 @default.
• W1525517607 countsByYear W15255176072013 @default.
• W1525517607 countsByYear W15255176072014 @default.
• W1525517607 countsByYear W15255176072015 @default.
• W1525517607 countsByYear W15255176072017 @default.
• W1525517607 countsByYear W15255176072020 @default.
• W1525517607 countsByYear W15255176072021 @default.
• W1525517607 countsByYear W15255176072023 @default.
• W1525517607 crossrefType "journal-article" @default.
• W1525517607 hasAuthorship W1525517607A5019234325 @default.
• W1525517607 hasAuthorship W1525517607A5056607779 @default.
• W1525517607 hasBestOaLocation W15255176071 @default.
• W1525517607 hasConcept C14036430 @default.
• W1525517607 hasConcept C178790620 @default.
• W1525517607 hasConcept C185592680 @default.
• W1525517607 hasConcept C201267052 @default.
• W1525517607 hasConcept C203014093 @default.
• W1525517607 hasConcept C2777292125 @default.
• W1525517607 hasConcept C2781287897 @default.
• W1525517607 hasConcept C3018972837 @default.
• W1525517607 hasConcept C519063684 @default.
• W1525517607 hasConcept C55493867 @default.
• W1525517607 hasConcept C86803240 @default.
• W1525517607 hasConcept C89560881 @default.
• W1525517607 hasConcept C95444343 @default.
• W1525517607 hasConceptScore W1525517607C14036430 @default.
• W1525517607 hasConceptScore W1525517607C178790620 @default.
• W1525517607 hasConceptScore W1525517607C185592680 @default.
• W1525517607 hasConceptScore W1525517607C201267052 @default.
• W1525517607 hasConceptScore W1525517607C203014093 @default.
• W1525517607 hasConceptScore W1525517607C2777292125 @default.
• W1525517607 hasConceptScore W1525517607C2781287897 @default.
• W1525517607 hasConceptScore W1525517607C3018972837 @default.
• W1525517607 hasConceptScore W1525517607C519063684 @default.
• W1525517607 hasConceptScore W1525517607C55493867 @default.
• W1525517607 hasConceptScore W1525517607C86803240 @default.
• W1525517607 hasConceptScore W1525517607C89560881 @default.
• W1525517607 hasConceptScore W1525517607C95444343 @default.
• W1525517607 hasIssue "36" @default.
• W1525517607 hasLocation W15255176071 @default.
• W1525517607 hasOpenAccess W1525517607 @default.
• W1525517607 hasPrimaryLocation W15255176071 @default.
• W1525517607 hasRelatedWork W2001243713 @default.
• W1525517607 hasRelatedWork W2073806240 @default.
• W1525517607 hasRelatedWork W2076916940 @default.
• W1525517607 hasRelatedWork W2089750436 @default.
• W1525517607 hasRelatedWork W2399776670 @default.
• W1525517607 hasRelatedWork W2411901489 @default.
• W1525517607 hasRelatedWork W2423592126 @default.

• next