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• W1525660737 abstract "The vaccinia virus gene A18R is essential for virus infection. The loss of A18R protein function results in unregulated transcription late during virus infection from regions of the viral genome which are normally transcriptionally quiescent. We have characterized A18R protein expression in cells infected with wild-type virus and the A18R temperature-sensitive mutant Cts23. The A18R protein is expressed during early and late phases of infection. The A18R protein expressed by Cts23 virus at permissive and nonpermissive temperatures is significantly less stable than the wild-type A18R protein. The A18R protein was identified as a virion component and localized by detergent extraction to the virion core. Virions purified from cells infected with the A18R temperature-sensitive mutants Cts4, Cts22, and Cts23 are defective in early viral transcription in vitro. The mutant transcription defect is not attributable to gross defects in virion structure or virion DNA-dependent RNA polymerase activity. We conclude that the A18R protein plays a role in viral transcription during the early phase of infection as well as during the late phase." @default.
• W1525660737 created "2016-06-24" @default.
• W1525660737 creator A5002964391 @default.
• W1525660737 creator A5008062442 @default.
• W1525660737 date "1994-06-01" @default.
• W1525660737 modified "2023-10-17" @default.
• W1525660737 title "The vaccinia virus A18R protein plays a role in viral transcription during both the early and the late phases of infection" @default.
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• W1525660737 doi "https://doi.org/10.1128/jvi.68.6.3642-3649.1994" @default.
• W1525660737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/236868" @default.
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• W1525660737 hasPublicationYear "1994" @default.
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