Matches in SemOpenAlex for { <https://semopenalex.org/work/W1525868044> ?p ?o ?g. }
- W1525868044 endingPage "4214" @default.
- W1525868044 startingPage "4206" @default.
- W1525868044 abstract "ABSTRACT Plasmodium falciparum is transmitted from humans to Anopheles mosquito vectors via the sexual erythrocytic forms termed gametocytes. Erythrocyte filtration through microsphere layers (microsphiltration) had shown that circulating gametocytes are deformable. Compounds reducing gametocyte deformability would induce their splenic clearance, thus removing them from the blood circulation and blocking malaria transmission. The hand-made, single-sample prototype for microsphiltration was miniaturized to a 96-well microtiter plate format, and gametocyte retention in the microsphere filters was quantified by high-content imaging. The stiffening activity of 40 pharmacological compounds was assessed in microtiter plates, using a small molecule (calyculin) as a positive control. The stiffening activity of calyculin was assessed in spleen-mimetic microfluidic chips and in macrophage-depleted mice. Marked mechanical retention (80% to 90%) of mature gametocytes was obtained in microplates following exposure to calyculin at concentrations with no effect on parasite viability. Of the 40 compounds tested, including 20 antimalarials, only 5 endoperoxides significantly increased gametocyte retention (1.5- to 2.5-fold; 24 h of exposure at 1 μM). Mature gametocytes exposed to calyculin accumulated in microfluidic chips and were cleared from the circulation of macrophage-depleted mice as rapidly as heat-stiffened erythrocytes, thus confirming results obtained using the microsphiltration assay. An automated miniaturized approach to select compounds for their gametocyte-stiffening effect has been established. Stiffening induces gametocyte clearance both in vitro and in vivo . Based on physiologically validated tools, this screening cascade can identify novel compounds and uncover new targets to block malaria transmission. Innovative applications in hematology are also envisioned." @default.
- W1525868044 created "2016-06-24" @default.
- W1525868044 creator A5003076755 @default.
- W1525868044 creator A5008341541 @default.
- W1525868044 creator A5010368343 @default.
- W1525868044 creator A5010742701 @default.
- W1525868044 creator A5012305166 @default.
- W1525868044 creator A5029382890 @default.
- W1525868044 creator A5029453857 @default.
- W1525868044 creator A5043673615 @default.
- W1525868044 creator A5043917716 @default.
- W1525868044 creator A5055080787 @default.
- W1525868044 creator A5059081442 @default.
- W1525868044 creator A5065889464 @default.
- W1525868044 creator A5068629830 @default.
- W1525868044 creator A5076084805 @default.
- W1525868044 creator A5087460582 @default.
- W1525868044 date "2015-07-01" @default.
- W1525868044 modified "2023-10-09" @default.
- W1525868044 title "Splenic Retention of Plasmodium falciparum Gametocytes To Block the Transmission of Malaria" @default.
- W1525868044 cites W1514025932 @default.
- W1525868044 cites W1531980086 @default.
- W1525868044 cites W1982297674 @default.
- W1525868044 cites W1982679597 @default.
- W1525868044 cites W1995485815 @default.
- W1525868044 cites W2011128579 @default.
- W1525868044 cites W2013792612 @default.
- W1525868044 cites W2022066534 @default.
- W1525868044 cites W2048368768 @default.
- W1525868044 cites W2048671997 @default.
- W1525868044 cites W2053758185 @default.
- W1525868044 cites W2062396541 @default.
- W1525868044 cites W2062627902 @default.
- W1525868044 cites W2067841560 @default.
- W1525868044 cites W2070251534 @default.
- W1525868044 cites W2081711923 @default.
- W1525868044 cites W2089837291 @default.
- W1525868044 cites W2090344797 @default.
- W1525868044 cites W2096937165 @default.
- W1525868044 cites W2101792514 @default.
- W1525868044 cites W2111166381 @default.
- W1525868044 cites W2112076496 @default.
- W1525868044 cites W2120482401 @default.
- W1525868044 cites W2121751652 @default.
- W1525868044 cites W2122753628 @default.
- W1525868044 cites W2124658904 @default.
- W1525868044 cites W2125306514 @default.
- W1525868044 cites W2125306573 @default.
- W1525868044 cites W2125851600 @default.
- W1525868044 cites W2135304792 @default.
- W1525868044 cites W2136461580 @default.
- W1525868044 cites W2138741610 @default.
- W1525868044 cites W2142817661 @default.
- W1525868044 cites W2147713260 @default.
- W1525868044 cites W2148868397 @default.
- W1525868044 cites W2155009848 @default.
- W1525868044 cites W2163816642 @default.
- W1525868044 cites W2166854800 @default.
- W1525868044 cites W2169303083 @default.
- W1525868044 cites W2171323885 @default.
- W1525868044 cites W2172062656 @default.
- W1525868044 cites W2262173735 @default.
- W1525868044 cites W2324280426 @default.
- W1525868044 cites W2413009189 @default.
- W1525868044 cites W4211007026 @default.
- W1525868044 cites W4211136995 @default.
- W1525868044 cites W4231646317 @default.
- W1525868044 cites W47133697 @default.
- W1525868044 doi "https://doi.org/10.1128/aac.05030-14" @default.
- W1525868044 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4468678" @default.
- W1525868044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25941228" @default.
- W1525868044 hasPublicationYear "2015" @default.
- W1525868044 type Work @default.
- W1525868044 sameAs 1525868044 @default.
- W1525868044 citedByCount "24" @default.
- W1525868044 countsByYear W15258680442015 @default.
- W1525868044 countsByYear W15258680442016 @default.
- W1525868044 countsByYear W15258680442017 @default.
- W1525868044 countsByYear W15258680442018 @default.
- W1525868044 countsByYear W15258680442019 @default.
- W1525868044 countsByYear W15258680442020 @default.
- W1525868044 countsByYear W15258680442021 @default.
- W1525868044 countsByYear W15258680442022 @default.
- W1525868044 countsByYear W15258680442023 @default.
- W1525868044 crossrefType "journal-article" @default.
- W1525868044 hasAuthorship W1525868044A5003076755 @default.
- W1525868044 hasAuthorship W1525868044A5008341541 @default.
- W1525868044 hasAuthorship W1525868044A5010368343 @default.
- W1525868044 hasAuthorship W1525868044A5010742701 @default.
- W1525868044 hasAuthorship W1525868044A5012305166 @default.
- W1525868044 hasAuthorship W1525868044A5029382890 @default.
- W1525868044 hasAuthorship W1525868044A5029453857 @default.
- W1525868044 hasAuthorship W1525868044A5043673615 @default.
- W1525868044 hasAuthorship W1525868044A5043917716 @default.
- W1525868044 hasAuthorship W1525868044A5055080787 @default.
- W1525868044 hasAuthorship W1525868044A5059081442 @default.
- W1525868044 hasAuthorship W1525868044A5065889464 @default.
- W1525868044 hasAuthorship W1525868044A5068629830 @default.