Matches in SemOpenAlex for { <https://semopenalex.org/work/W1526010080> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W1526010080 endingPage "3523" @default.
- W1526010080 startingPage "3516" @default.
- W1526010080 abstract "Abstract Induction of mouse peritoneal macrophage cytotoxicity against SV3T3, a line of virally transformed mouse cells correlated with the distribution of cytoplasmic calmodulin in the macrophages. The organization of the cytoskeleton was examined by fluorescent microscopy and by transmission electron microscopy, using immunogold tagging after Triton-X-100 (TX-100) extraction of the macrophages. Macrophages that had been activated to a tumoricidal state in vivo by vaccinia virus or in vitro by lymphokine stimulation displayed cytoskeletal networks that were more extended and weblike than did resident macrophages. The organization of microfilaments and microtubules in the cytoskeleton was displayed by using either anti-actin or anti-tubulin. Immunogold labeling of tumoricidal macrophage cytoskeletons with anti-calmodulin revealed strong binding to the microfilament network and no binding to microtubules. Anti-calmodulin reacted weakly with the cytoskeletal network of resident macrophages, and this was not demonstrably greater than the reaction with normal sheep serum. However, resident macrophages displayed a high density of calmodulin (CAM) associated with unidentifiable structures in the perinuclear region when reacted with anti-calmodulin. These characteristic distributions of CAM in resident and activated macrophages was confirmed by immunofluorescence. The total and cytoskeletal-associated amounts of calmodulin per unit of protein were determined by radioimmune assay and 125I labeling followed by SDS-PAGE. No statistically significant differences were detected between resident and activated macrophages in either the total cell or cytoskeleton fractions. In summary, our results suggest that induction of tumoricidal activity of mouse peritoneal macrophages correlates with the translocation of calmodulin to the microfilament network of the cytoskeleton." @default.
- W1526010080 created "2016-06-24" @default.
- W1526010080 creator A5000679376 @default.
- W1526010080 creator A5021197625 @default.
- W1526010080 creator A5034792642 @default.
- W1526010080 creator A5036288870 @default.
- W1526010080 creator A5050594568 @default.
- W1526010080 creator A5056374872 @default.
- W1526010080 date "1985-05-01" @default.
- W1526010080 modified "2023-10-04" @default.
- W1526010080 title "Binding of calmodulin to the microfilament network correlates with induction of a macrophage tumoricidal response." @default.
- W1526010080 doi "https://doi.org/10.4049/jimmunol.134.5.3516" @default.
- W1526010080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3884713" @default.
- W1526010080 hasPublicationYear "1985" @default.
- W1526010080 type Work @default.
- W1526010080 sameAs 1526010080 @default.
- W1526010080 citedByCount "10" @default.
- W1526010080 crossrefType "journal-article" @default.
- W1526010080 hasAuthorship W1526010080A5000679376 @default.
- W1526010080 hasAuthorship W1526010080A5021197625 @default.
- W1526010080 hasAuthorship W1526010080A5034792642 @default.
- W1526010080 hasAuthorship W1526010080A5036288870 @default.
- W1526010080 hasAuthorship W1526010080A5050594568 @default.
- W1526010080 hasAuthorship W1526010080A5056374872 @default.
- W1526010080 hasBestOaLocation W15260100801 @default.
- W1526010080 hasConcept C125705527 @default.
- W1526010080 hasConcept C142669718 @default.
- W1526010080 hasConcept C1491633281 @default.
- W1526010080 hasConcept C159654299 @default.
- W1526010080 hasConcept C181199279 @default.
- W1526010080 hasConcept C189040839 @default.
- W1526010080 hasConcept C202751555 @default.
- W1526010080 hasConcept C203014093 @default.
- W1526010080 hasConcept C20418707 @default.
- W1526010080 hasConcept C2779244956 @default.
- W1526010080 hasConcept C29688787 @default.
- W1526010080 hasConcept C30030713 @default.
- W1526010080 hasConcept C55493867 @default.
- W1526010080 hasConcept C86803240 @default.
- W1526010080 hasConcept C95444343 @default.
- W1526010080 hasConceptScore W1526010080C125705527 @default.
- W1526010080 hasConceptScore W1526010080C142669718 @default.
- W1526010080 hasConceptScore W1526010080C1491633281 @default.
- W1526010080 hasConceptScore W1526010080C159654299 @default.
- W1526010080 hasConceptScore W1526010080C181199279 @default.
- W1526010080 hasConceptScore W1526010080C189040839 @default.
- W1526010080 hasConceptScore W1526010080C202751555 @default.
- W1526010080 hasConceptScore W1526010080C203014093 @default.
- W1526010080 hasConceptScore W1526010080C20418707 @default.
- W1526010080 hasConceptScore W1526010080C2779244956 @default.
- W1526010080 hasConceptScore W1526010080C29688787 @default.
- W1526010080 hasConceptScore W1526010080C30030713 @default.
- W1526010080 hasConceptScore W1526010080C55493867 @default.
- W1526010080 hasConceptScore W1526010080C86803240 @default.
- W1526010080 hasConceptScore W1526010080C95444343 @default.
- W1526010080 hasIssue "5" @default.
- W1526010080 hasLocation W15260100801 @default.
- W1526010080 hasLocation W15260100802 @default.
- W1526010080 hasOpenAccess W1526010080 @default.
- W1526010080 hasPrimaryLocation W15260100801 @default.
- W1526010080 hasRelatedWork W144838933 @default.
- W1526010080 hasRelatedWork W1531816097 @default.
- W1526010080 hasRelatedWork W1976433348 @default.
- W1526010080 hasRelatedWork W2050736627 @default.
- W1526010080 hasRelatedWork W2061887935 @default.
- W1526010080 hasRelatedWork W2081451190 @default.
- W1526010080 hasRelatedWork W2082302791 @default.
- W1526010080 hasRelatedWork W2352927845 @default.
- W1526010080 hasRelatedWork W2471276139 @default.
- W1526010080 hasRelatedWork W4245474806 @default.
- W1526010080 hasVolume "134" @default.
- W1526010080 isParatext "false" @default.
- W1526010080 isRetracted "false" @default.
- W1526010080 magId "1526010080" @default.
- W1526010080 workType "article" @default.