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- W1526093314 abstract "The chapter uses a series of ocular tissues to demonstrate how intact or thick specimens can be prepared and analyzed using confocal microscopy and computerized three-dimensional (3-D) reconstruction. The chapter describes protocols for the use of histochemical and immunocytochemical reagents for analysis of specimens by confocal microscopy. The descriptions given in tables relate to analysis of ophthalmic tissue, a particular area of research interest. The chapter describes the use of picrosirius red (PSR) for the analysis of connective tissue in thick sections of the optic nerve. Connective tissue, such as collagen and elastin, is known to be present along the length of the optic nerve, where it provides structural support to the axon bundles that transmit visual impulses from the retina to the brain. Accurate analysis of the 3-D structure of biological specimens using confocal microscopy and computerized 3-D reconstruction first requires that the original 3-D data sets are optimally collected and processed. The combined use of these stereological tools with confocal microscopy and computerized 3-D reconstruction will improve the understanding of structural features and relationships that occur within normal and diseased tissues." @default.
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- W1526093314 date "1999-01-01" @default.
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- W1526093314 title "[28] Characterization of ocular cellular and extracellular structures using confocal microscopy and computerized three-dimensional reconstruction" @default.
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- W1526093314 doi "https://doi.org/10.1016/s0076-6879(99)07030-5" @default.
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