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- W1526507834 abstract "1. The distribution of phosphatidic acid phosphatase is studied in the liver, kidney, brain, and intestinal mucosa of the ox, pig, rabbit, guinea-pig, and rat. Cell fractionation of pig kidney revealed that 63% of the total activity is in the microsomal fraction. 2. The microsomal enzyme from pig kidney is particulate, shows a pH optimum at 6.0, has a Km of 2.2·10−4 for phosphatidic acid, and is inhibited by fluoride ions. SH groups of the enzyme seem to be essential for activity. 3. Using the microsomal enzyme, the effect of metal ions, chelating agents, detergents, some hydrolytic enzymes, autolysis, and of some higher alcohols is investigated. 4. Procedures for the partial purification and solubilisation of the enzyme are described. The purified enzyme is highly specific for phosphatidic acid and hydrolyses only the phosphoric acid ester bond." @default.
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- W1526507834 date "1962-01-01" @default.
- W1526507834 modified "2023-09-30" @default.
- W1526507834 title "Metabolism of phospholipids" @default.
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- W1526507834 doi "https://doi.org/10.1016/0006-3002(62)90600-5" @default.
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