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- W1526987889 abstract "Abstract De novo synthesis of long double‐stranded DNA constructs has a myriad of applications in biology and biological engineering. However, its widespread adoption has been hindered by high costs. Cost can be significantly reduced by using oligonucleotides synthesized on high‐density DNA chips. However, most methods for using off‐chip DNA for gene synthesis have failed to scale due to the high error rates, low yields, and high chemical complexity of the chip‐synthesized oligonucleotides. We have recently demonstrated that some commercial DNA chip manufacturers have improved error rates, and that the issues of chemical complexity and low yields can be solved by using barcoded primers to accurately and efficiently amplify subpools of oligonucleotides. This unit includes protocols for computationally designing the DNA chip, amplifying the oligonucleotide subpools, and assembling 500‐ to 800‐bp constructs. Curr. Protoc. Chem. Biol . 4:1‐17 © 2012 by John Wiley & Sons, Inc." @default.
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- W1526987889 date "2012-03-01" @default.
- W1526987889 modified "2023-10-11" @default.
- W1526987889 title "Gene Assembly from Chip‐Synthesized Oligonucleotides" @default.
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- W1526987889 doi "https://doi.org/10.1002/9780470559277.ch110190" @default.
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