FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1527960670> ?p ?o ?g. }

• W1527960670 endingPage "1756" @default.
• W1527960670 startingPage "1748" @default.
• W1527960670 abstract "Type IIa bacteriocins, which are isolated from lactic acid bacteria that are useful for food preservation, are potent antimicrobial peptides with considerable potential as therapeutic agents for gastrointestinal infections in mammals. They are ribosomally synthesized as precursors with an N-terminal leader, typically 18-24 amino acid residues in length, which is cleaved during export from the producing cell. We have chemically synthesized the full precursor of carnobacteriocin B2, precarnobacteriocin (preCbnB2), which has a C-terminal amide rather than a carboxyl, and also produced preCbnB2(1-64), which is missing two amino acid residues at the C-terminus (Arg65 and Pro66), via expression in Escherichia coli as a maltose-binding protein fusion that is then cut with Factor Xa. PreCbnB2(1-64) is readily labeled with (15)N and (13)C for NMR studies using the latter approach. Multidimensional NMR analysis of preCbnB2(1-64) shows that, like the parent bacteriocin, it exists as a random coil in water but assumes a defined conformation in water/trifluoroethanol mixtures. In 70 : 30 trifluoroethanol/water, the 3D structure of the preCbnB2 section corresponding to the mature bacteriocin is essentially the same as reported previously by us for carnobacteriocin B2 (CbnB2). This structure maintains the highly conserved alpha-helix corresponding to residues 20-38 of CbnB2 that is believed to be responsible for interaction with a target receptor in sensitive cells, including Listeria monocytogenes. PreCbnB2 also has a second alpha-helix from residues 3-13 (i.e. -15 to -5 relative to CbnB2) in the leader section of the peptide. This helix appears to be conserved in related type IIa bacteriocin precursors based on sequence analysis. It is likely to be a key recognition element for export and processing, and is probably responsible for the considerably reduced antimicrobial activity of preCbnB2. The latter effect may assist the producing cell in avoiding the toxic effects of the bacteriocin. This is the first 3D structure determined for a prebacteriocin from lactic acid bacteria." @default.
• W1527960670 created "2016-06-24" @default.
• W1527960670 creator A5003573061 @default.
• W1527960670 creator A5007325812 @default.
• W1527960670 creator A5010530966 @default.
• W1527960670 creator A5037621647 @default.
• W1527960670 creator A5042573551 @default.
• W1527960670 date "2004-05-01" @default.
• W1527960670 modified "2023-10-12" @default.
• W1527960670 title "NMR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide from Carnobacterium piscicola. Implications of the alpha-helical leader section for export and inhibition of type IIa bacteriocin activity" @default.
• W1527960670 cites W130146406 @default.
• W1527960670 cites W1509140125 @default.
• W1527960670 cites W1638851988 @default.
• W1527960670 cites W1727914715 @default.
• W1527960670 cites W1889960381 @default.
• W1527960670 cites W1929740025 @default.
• W1527960670 cites W1966120465 @default.
• W1527960670 cites W1973259178 @default.
• W1527960670 cites W1984683656 @default.
• W1527960670 cites W1986191025 @default.
• W1527960670 cites W1995017064 @default.
• W1527960670 cites W1995601636 @default.
• W1527960670 cites W1999291944 @default.
• W1527960670 cites W2003219033 @default.
• W1527960670 cites W2003340039 @default.
• W1527960670 cites W2006452034 @default.
• W1527960670 cites W2012689130 @default.
• W1527960670 cites W2015032046 @default.
• W1527960670 cites W2022162043 @default.
• W1527960670 cites W2030433731 @default.
• W1527960670 cites W2035218417 @default.
• W1527960670 cites W2043172297 @default.
• W1527960670 cites W2047696520 @default.
• W1527960670 cites W2057138984 @default.
• W1527960670 cites W2061144516 @default.
• W1527960670 cites W2068227670 @default.
• W1527960670 cites W2069602336 @default.
• W1527960670 cites W2082176639 @default.
• W1527960670 cites W2083789703 @default.
• W1527960670 cites W2085998427 @default.
• W1527960670 cites W2099858678 @default.
• W1527960670 cites W2109077033 @default.
• W1527960670 cites W2117207469 @default.
• W1527960670 cites W2123119918 @default.
• W1527960670 cites W2130332666 @default.
• W1527960670 cites W2133338325 @default.
• W1527960670 cites W2139021858 @default.
• W1527960670 cites W2142340262 @default.
• W1527960670 cites W2162623537 @default.
• W1527960670 cites W2163454941 @default.
• W1527960670 cites W2169821755 @default.
• W1527960670 cites W2170008914 @default.
• W1527960670 cites W4214939930 @default.
• W1527960670 cites W4246525150 @default.
• W1527960670 doi "https://doi.org/10.1111/j.1432-1033.2004.04085.x" @default.
• W1527960670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15096213" @default.
• W1527960670 hasPublicationYear "2004" @default.
• W1527960670 type Work @default.
• W1527960670 sameAs 1527960670 @default.
• W1527960670 citedByCount "35" @default.
• W1527960670 countsByYear W15279606702012 @default.
• W1527960670 countsByYear W15279606702013 @default.
• W1527960670 countsByYear W15279606702014 @default.
• W1527960670 countsByYear W15279606702016 @default.
• W1527960670 countsByYear W15279606702018 @default.
• W1527960670 countsByYear W15279606702019 @default.
• W1527960670 countsByYear W15279606702021 @default.
• W1527960670 countsByYear W15279606702022 @default.
• W1527960670 countsByYear W15279606702023 @default.
• W1527960670 crossrefType "journal-article" @default.
• W1527960670 hasAuthorship W1527960670A5003573061 @default.
• W1527960670 hasAuthorship W1527960670A5007325812 @default.
• W1527960670 hasAuthorship W1527960670A5010530966 @default.
• W1527960670 hasAuthorship W1527960670A5037621647 @default.
• W1527960670 hasAuthorship W1527960670A5042573551 @default.
• W1527960670 hasBestOaLocation W15279606701 @default.
• W1527960670 hasConcept C104317684 @default.
• W1527960670 hasConcept C115574571 @default.
• W1527960670 hasConcept C167625842 @default.
• W1527960670 hasConcept C178790620 @default.
• W1527960670 hasConcept C185592680 @default.
• W1527960670 hasConcept C2779281246 @default.
• W1527960670 hasConcept C34349673 @default.
• W1527960670 hasConcept C4937899 @default.
• W1527960670 hasConcept C515207424 @default.
• W1527960670 hasConcept C540938839 @default.
• W1527960670 hasConcept C547475151 @default.
• W1527960670 hasConcept C55493867 @default.
• W1527960670 hasConcept C62614982 @default.
• W1527960670 hasConcept C66974803 @default.
• W1527960670 hasConcept C71240020 @default.
• W1527960670 hasConceptScore W1527960670C104317684 @default.
• W1527960670 hasConceptScore W1527960670C115574571 @default.
• W1527960670 hasConceptScore W1527960670C167625842 @default.
• W1527960670 hasConceptScore W1527960670C178790620 @default.
• W1527960670 hasConceptScore W1527960670C185592680 @default.
• W1527960670 hasConceptScore W1527960670C2779281246 @default.
• W1527960670 hasConceptScore W1527960670C34349673 @default.

• next