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- W152800948 abstract "Publisher Summary This chapter focuses on the application of spin labeling technique to membrane-bound enzymes. The technique is the epr spin labeling method was developed by H.M. McConnel and is based on the use of stable free radicals called nitroxides. The theory suggests that in the presence of a magnetic field, the spin angular momentum of an electron is quantized. The chapter presents a useful case of anisotropic motion of spin labels is axially symmetric motion. The spin labeling studies shows that denaturation by alcohols proceeds by disruption of the phospholipid bilayer with no major disruption of protein structure. Denaturation by guanidine HC1 disrupts protein structure with little effect on phospholipid structure. Sodium dodecyl sulfate disrupts neither protein nor phospholipid. Another spin label that may be useful is the amidinonitroxide VII. This lipophilic label has a pKa of near 7, and on protonation shows a dramatic change in its epr structure. The label should be useful as a probe of pH changes at the membrane surface." @default.
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- W152800948 date "1974-01-01" @default.
- W152800948 modified "2023-09-24" @default.
- W152800948 title "APPLICATION OF THE SPIN LABELING TECHNIQUE TO BIOLOGICAL MEMBRANES" @default.
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- W152800948 doi "https://doi.org/10.1016/b978-0-12-107950-5.50007-2" @default.
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