FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1528052802> ?p ?o ?g. }

• W1528052802 endingPage "46" @default.
• W1528052802 startingPage "29" @default.
• W1528052802 abstract "The cloning of DNA fragments into vectors or host genomes has traditionally been performed using Escherichia coli with restriction enzymes and DNA ligase or homologous recombination-based reactions. We report here a novel DNA cloning method that does not require DNA end processing or homologous recombination, but that ensures highly accurate cloning. The method exploits the efficient non-homologous end-joining (NHEJ) activity of the yeast Kluyveromyces marxianus and consists of a novel functional marker selection system. First, to demonstrate the applicability of NHEJ to DNA cloning, a C-terminal-truncated non-functional ura3 selection marker and the truncated region were PCR-amplified separately, mixed and directly used for the transformation. URA3(+) transformants appeared on the selection plates, indicating that the two DNA fragments were correctly joined by NHEJ to generate a functional URA3 gene that had inserted into the yeast chromosome. To develop the cloning system, the shortest URA3 C-terminal encoding sequence that could restore the function of a truncated non-functional ura3 was determined by deletion analysis, and was included in the primers to amplify target DNAs for cloning. Transformation with PCR-amplified target DNAs and C-terminal truncated ura3 produced numerous transformant colonies, in which a functional URA3 gene was generated and was integrated into the chromosome with the target DNAs. Several K. marxianus circular plasmids with different selection markers were also developed for NHEJ-based cloning and recombinant DNA construction. The one-step DNA cloning method developed here is a relatively simple and reliable procedure among the DNA cloning systems developed to date." @default.
• W1528052802 created "2016-06-24" @default.
• W1528052802 creator A5010627311 @default.
• W1528052802 creator A5011662830 @default.
• W1528052802 creator A5028862416 @default.
• W1528052802 creator A5041379019 @default.
• W1528052802 creator A5046391255 @default.
• W1528052802 creator A5048623060 @default.
• W1528052802 creator A5050840503 @default.
• W1528052802 creator A5086214931 @default.
• W1528052802 creator A5090411353 @default.
• W1528052802 date "2013-12-19" @default.
• W1528052802 modified "2023-10-06" @default.
• W1528052802 title "Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast<i>Kluyveromyces marxianus</i>" @default.
• W1528052802 cites W1487130044 @default.
• W1528052802 cites W14875095 @default.
• W1528052802 cites W1517699507 @default.
• W1528052802 cites W1542802788 @default.
• W1528052802 cites W1559808711 @default.
• W1528052802 cites W1881426718 @default.
• W1528052802 cites W1940574227 @default.
• W1528052802 cites W1968143878 @default.
• W1528052802 cites W1975425948 @default.
• W1528052802 cites W1978676779 @default.
• W1528052802 cites W1995953468 @default.
• W1528052802 cites W1996023701 @default.
• W1528052802 cites W1997972699 @default.
• W1528052802 cites W1998592297 @default.
• W1528052802 cites W2003581594 @default.
• W1528052802 cites W2007049021 @default.
• W1528052802 cites W2010939464 @default.
• W1528052802 cites W2013947447 @default.
• W1528052802 cites W2021845150 @default.
• W1528052802 cites W2027625002 @default.
• W1528052802 cites W2033896965 @default.
• W1528052802 cites W2040108963 @default.
• W1528052802 cites W2041509866 @default.
• W1528052802 cites W2041746508 @default.
• W1528052802 cites W2057967928 @default.
• W1528052802 cites W2059464705 @default.
• W1528052802 cites W2073082596 @default.
• W1528052802 cites W2075075690 @default.
• W1528052802 cites W2078591700 @default.
• W1528052802 cites W2080408951 @default.
• W1528052802 cites W2090015639 @default.
• W1528052802 cites W2095904508 @default.
• W1528052802 cites W2100057937 @default.
• W1528052802 cites W2107006351 @default.
• W1528052802 cites W2116207915 @default.
• W1528052802 cites W2122043909 @default.
• W1528052802 cites W2127073167 @default.
• W1528052802 cites W2136451426 @default.
• W1528052802 cites W2137082459 @default.
• W1528052802 cites W2146503498 @default.
• W1528052802 cites W2155692678 @default.
• W1528052802 cites W2156715038 @default.
• W1528052802 cites W2164425287 @default.
• W1528052802 cites W2169333620 @default.
• W1528052802 cites W2169565570 @default.
• W1528052802 cites W2169805130 @default.
• W1528052802 cites W33972585 @default.
• W1528052802 cites W4230565692 @default.
• W1528052802 cites W52154540 @default.
• W1528052802 cites W6375506 @default.
• W1528052802 cites W8074104 @default.
• W1528052802 doi "https://doi.org/10.1002/yea.2993" @default.
• W1528052802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24307396" @default.
• W1528052802 hasPublicationYear "2013" @default.
• W1528052802 type Work @default.
• W1528052802 sameAs 1528052802 @default.
• W1528052802 citedByCount "34" @default.
• W1528052802 countsByYear W15280528022014 @default.
• W1528052802 countsByYear W15280528022015 @default.
• W1528052802 countsByYear W15280528022016 @default.
• W1528052802 countsByYear W15280528022017 @default.
• W1528052802 countsByYear W15280528022018 @default.
• W1528052802 countsByYear W15280528022019 @default.
• W1528052802 countsByYear W15280528022020 @default.
• W1528052802 countsByYear W15280528022021 @default.
• W1528052802 countsByYear W15280528022022 @default.
• W1528052802 countsByYear W15280528022023 @default.
• W1528052802 crossrefType "journal-article" @default.
• W1528052802 hasAuthorship W1528052802A5010627311 @default.
• W1528052802 hasAuthorship W1528052802A5011662830 @default.
• W1528052802 hasAuthorship W1528052802A5028862416 @default.
• W1528052802 hasAuthorship W1528052802A5041379019 @default.
• W1528052802 hasAuthorship W1528052802A5046391255 @default.
• W1528052802 hasAuthorship W1528052802A5048623060 @default.
• W1528052802 hasAuthorship W1528052802A5050840503 @default.
• W1528052802 hasAuthorship W1528052802A5086214931 @default.
• W1528052802 hasAuthorship W1528052802A5090411353 @default.
• W1528052802 hasBestOaLocation W15280528021 @default.
• W1528052802 hasConcept C102744134 @default.
• W1528052802 hasConcept C104317684 @default.
• W1528052802 hasConcept C107618985 @default.
• W1528052802 hasConcept C121050878 @default.
• W1528052802 hasConcept C153911025 @default.
• W1528052802 hasConcept C168374021 @default.

• next