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- W1528144502 abstract "Abstract A new sensor is decribed for a specific protein; enzyme-linked immunosorbent assays are combined with electrochemical measurements. Specific sensors are proposed based on immobilization of an antibody by ligands onto artificial protein-based membranes, combined with a computerized system, for the determination of various antigens or haptens. Specific antibodies labelled by ribonuclease are reversibly bound to the membrane by using cysteine as the ligand. Two enzymes are used: ribonuclease is used for reversibly linking the immuno-complex to the insoluble matrix via disulfide bridges; β- d -glucose oxidase is used for labelling the antigen. The measurement consists of an immuno-logical process and an enzymatic reaction. The protein-based membrane activated with thiol groups is fixed over an oxygen electrode. After incubation of the free antigen and the antigen labelled with glucose oxidase with specific antibodies linked by ribonuclease, the reaction medium is introduced in a continuous flow cell. The oxygen consumption by the enzyme reaction is measured on-line with the electrode in contact with a standard glucose solution. This response is correlated to the antigen concentration of the sample. The signal is directly proportional to the oxygen consumption. The reproducibility with use of the same membrane is" @default.
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- W1528144502 date "1987-01-01" @default.
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- W1528144502 title "Reversible immobilization of an antibody with a thiol-substituted sorbent" @default.
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- W1528144502 doi "https://doi.org/10.1016/s0003-2670(00)84731-5" @default.
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