Matches in SemOpenAlex for { <https://semopenalex.org/work/W1528208346> ?p ?o ?g. }
- W1528208346 endingPage "21559" @default.
- W1528208346 startingPage "21553" @default.
- W1528208346 abstract "Omeprazole and E3810 were found to inhibit gastric H+,K(+)-ATPase following different biochemical mechanisms. Effects of the specific binding of the inhibitors on the conformational state of the enzyme were studied by measuring the fluorescence of the enzyme labeled with fluorescein 5'-isothiocyanate. The absolute fluorescence level of the omeprazole-bound enzyme was lower than that of the control enzyme, and reduction of S-S cross-linking between the enzyme and omeprazole increased the fluorescence. Addition of K+ into the control vesicle solution quenched the fluorescence (E1-->E2K+). The quench was inhibited in the omeprazole-bound enzyme but not in the E3810-bound enzyme. These results suggest that the omeprazole-bound enzyme has a low fluorescence conformation (E2 form). On the other hand, the conformation of the E3810-bound enzyme was the same as that of the control enzyme (E1 form). Phosphoenzyme formation in the absence of K+ was inhibited in both the E3810- and omeprazole-bound enzymes. Binding of 2',3'-o-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate to the enzyme was equally inhibited by E3810 and omeprazole. K(+)-dependent dephosphorylation from the phosphoenzyme was inhibited in the E3810-bound enzyme but not in the omeprazole-bound enzyme. These experimental results have shown that the inhibition mechanism of H+,K(+)-ATPase by omeprazole was different from that by E3810; the partial reaction that was the most differently affected by the inhibitors was the conformational change from the E2 to E1 form for omeprazole and the luminal K(+)-dependent dephosphorylation for E3810." @default.
- W1528208346 created "2016-06-24" @default.
- W1528208346 creator A5015038980 @default.
- W1528208346 creator A5036123924 @default.
- W1528208346 date "1993-10-01" @default.
- W1528208346 modified "2023-09-28" @default.
- W1528208346 title "Different biochemical modes of action of two irreversible H+,K(+)-ATPase inhibitors, omeprazole and E3810." @default.
- W1528208346 cites W1482638264 @default.
- W1528208346 cites W1490607841 @default.
- W1528208346 cites W1492663625 @default.
- W1528208346 cites W1495357536 @default.
- W1528208346 cites W1513710592 @default.
- W1528208346 cites W1516001873 @default.
- W1528208346 cites W1539696604 @default.
- W1528208346 cites W1542845577 @default.
- W1528208346 cites W1554578020 @default.
- W1528208346 cites W1559742800 @default.
- W1528208346 cites W1572051922 @default.
- W1528208346 cites W1574349607 @default.
- W1528208346 cites W1579824808 @default.
- W1528208346 cites W1583022528 @default.
- W1528208346 cites W1585677287 @default.
- W1528208346 cites W1588028833 @default.
- W1528208346 cites W1593706770 @default.
- W1528208346 cites W1596185709 @default.
- W1528208346 cites W1602271438 @default.
- W1528208346 cites W1602506816 @default.
- W1528208346 cites W1671666965 @default.
- W1528208346 cites W1775749144 @default.
- W1528208346 cites W1971688135 @default.
- W1528208346 cites W1979087886 @default.
- W1528208346 cites W2005009549 @default.
- W1528208346 cites W2009346539 @default.
- W1528208346 cites W2009740509 @default.
- W1528208346 cites W2014433662 @default.
- W1528208346 cites W2021345688 @default.
- W1528208346 cites W2034257145 @default.
- W1528208346 cites W2036739534 @default.
- W1528208346 cites W2046075488 @default.
- W1528208346 cites W2050494502 @default.
- W1528208346 cites W2053171951 @default.
- W1528208346 cites W2057001748 @default.
- W1528208346 cites W2064210744 @default.
- W1528208346 cites W2066596442 @default.
- W1528208346 cites W2077613046 @default.
- W1528208346 cites W2104654345 @default.
- W1528208346 cites W2408765362 @default.
- W1528208346 cites W2414371662 @default.
- W1528208346 cites W50475275 @default.
- W1528208346 doi "https://doi.org/10.1016/s0021-9258(20)80577-8" @default.
- W1528208346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8408006" @default.
- W1528208346 hasPublicationYear "1993" @default.
- W1528208346 type Work @default.
- W1528208346 sameAs 1528208346 @default.
- W1528208346 citedByCount "37" @default.
- W1528208346 countsByYear W15282083462012 @default.
- W1528208346 countsByYear W15282083462013 @default.
- W1528208346 countsByYear W15282083462015 @default.
- W1528208346 countsByYear W15282083462016 @default.
- W1528208346 countsByYear W15282083462017 @default.
- W1528208346 crossrefType "journal-article" @default.
- W1528208346 hasAuthorship W1528208346A5015038980 @default.
- W1528208346 hasAuthorship W1528208346A5036123924 @default.
- W1528208346 hasBestOaLocation W15282083461 @default.
- W1528208346 hasConcept C121332964 @default.
- W1528208346 hasConcept C181199279 @default.
- W1528208346 hasConcept C185592680 @default.
- W1528208346 hasConcept C23265538 @default.
- W1528208346 hasConcept C2777498785 @default.
- W1528208346 hasConcept C2780791683 @default.
- W1528208346 hasConcept C55493867 @default.
- W1528208346 hasConcept C62520636 @default.
- W1528208346 hasConcept C86803240 @default.
- W1528208346 hasConcept C98274493 @default.
- W1528208346 hasConceptScore W1528208346C121332964 @default.
- W1528208346 hasConceptScore W1528208346C181199279 @default.
- W1528208346 hasConceptScore W1528208346C185592680 @default.
- W1528208346 hasConceptScore W1528208346C23265538 @default.
- W1528208346 hasConceptScore W1528208346C2777498785 @default.
- W1528208346 hasConceptScore W1528208346C2780791683 @default.
- W1528208346 hasConceptScore W1528208346C55493867 @default.
- W1528208346 hasConceptScore W1528208346C62520636 @default.
- W1528208346 hasConceptScore W1528208346C86803240 @default.
- W1528208346 hasConceptScore W1528208346C98274493 @default.
- W1528208346 hasIssue "29" @default.
- W1528208346 hasLocation W15282083461 @default.
- W1528208346 hasOpenAccess W1528208346 @default.
- W1528208346 hasPrimaryLocation W15282083461 @default.
- W1528208346 hasRelatedWork W1155355392 @default.
- W1528208346 hasRelatedWork W1989419945 @default.
- W1528208346 hasRelatedWork W1995672127 @default.
- W1528208346 hasRelatedWork W2060043280 @default.
- W1528208346 hasRelatedWork W2116185206 @default.
- W1528208346 hasRelatedWork W2959217856 @default.
- W1528208346 hasRelatedWork W3093028580 @default.
- W1528208346 hasRelatedWork W4214886483 @default.
- W1528208346 hasRelatedWork W4240721065 @default.
- W1528208346 hasRelatedWork W4377967821 @default.