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• W1528269861 abstract "There is increasing complexity in the organization of seven transmembrane domain (7TM) receptor signalling pathways, and in the ability of their ligands to modulate and direct this signalling. Underlying these events is a network of protein interactions between the 7TM receptors themselves and associated effectors, such as G proteins and beta-arrestins. Bimolecular fluorescence complementation, or BiFC, is a technique capable of detecting these protein-protein events essential for 7TM receptor function. Fluorescent proteins, such as those from Aequorea victoria, are split into two non-fluorescent halves, which then tag the proteins under study. On association, these fragments refold and regenerate a mature fluorescent protein, producing a BiFC signal indicative of complex formation. Here, we review the experimental criteria for successful application of BiFC, considered in the context of 7TM receptor signalling events such as receptor dimerization, G protein and beta-arrestin signalling. The advantages and limitations of BiFC imaging are compared with alternative resonance energy transfer techniques. We show that the essential simplicity of the fluorescent BiFC measurement allows high-content and advanced imaging applications, and that it can probe more complex multi-protein interactions alone or in combination with resonance energy transfer. These capabilities suggest that BiFC techniques will become ever more useful in the analysis of ligand and 7TM receptor pharmacology at the molecular level of protein-protein interactions." @default.
• W1528269861 created "2016-06-24" @default.
• W1528269861 creator A5009862868 @default.
• W1528269861 creator A5070048387 @default.
• W1528269861 creator A5076927839 @default.
• W1528269861 date "2009-12-10" @default.
• W1528269861 modified "2023-09-27" @default.
• W1528269861 title "Bimolecular fluorescence complementation: lighting up seven transmembrane domain receptor signalling networks" @default.
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• W1528269861 doi "https://doi.org/10.1111/j.1476-5381.2009.00480.x" @default.
• W1528269861 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2829200" @default.
• W1528269861 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20015298" @default.
• W1528269861 hasPublicationYear "2009" @default.
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