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- W152833152 abstract "Making wheat plants with novel seed protein composition is still a very laborious and time consuming task, so it is advisable to gather as much information as possible about a particular protein molecule before transferring its gene into the wheat genome. Various biochemical and biophysical methods are available for the in vitro study of the molecular structures and interactions of individual gluten proteins and their roles in determining the functional properties of gluten. Here, we report on how heterologous expression, a recently developed method for protein incorporation into gluten and a set of pilot scale equipment can be used to evaluate properties of wild-type and mutant proteins before introducing them into wheat. The structure of a storage protein gene was manipulated, using recombinant DNA technology, to change the number and distribution of cysteine residues in the molecule. Proteins from these genes were produced in a well established Escherichia coli expression system. Functional studies were carried out on purified proteins using the 2g Mixograph, small-scale Extensograph and a method called micro-baking. Transgenic plants were verified both at the DNA and protein levels. Functional data of the same proteins expressed in bacteria and in transgenic plants will be presented." @default.
- W152833152 created "2016-06-24" @default.
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- W152833152 date "2001-01-01" @default.
- W152833152 modified "2023-10-07" @default.
- W152833152 title "Small Scale Dough Testing as a Research Tool for Evaluating Properties of Single Storage Proteins Chosen for Wheat Transformation" @default.
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- W152833152 doi "https://doi.org/10.1007/978-94-017-3674-9_36" @default.
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