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- W1528649928 abstract "Rats weighing about 200 g were force-fed diets containing amino acid mixtures proportioned as in casein (21% of diet) but with varying amounts of methionine for a 3-day period. One group received a complete amino acid mixture (CAA). Other groups were given CAA diet devoid of methionine and cystine (-S) or CAA diet supplemented with 2% (+2% Met) or 4% (+4% Met) methionine. Plasma, liver and gastrocnemius muscle of the three groups of animals were analyzed for amino acids, and various hepatic amino acid-degrading enzymes were measured. With the -S diet as compared to the CAA diet, the methionine content of the tissues was similar but the cystine level in plasma was greatly decreased, and some other amino acids were increased, as for example, threonine and arginine. With the +4% Met diet as compared to the CAA diet, methionine was elevated in all tissues but a number of other amino acids were decreased including ornithine, arginine, glycine and glutamic acid. The activity of the amino acid-degrading enzymes in liver tissue tended to be lower with -S diets than with CAA diets and higher with +4% Met diets. For the -S diets a low threonine dehydratase activity was associated with high tissue concentrations of threonine and serine. The converse was found with +4% Met diet: high enzyme activity and low concentrations of the same amino acids." @default.
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- W1528649928 date "1969-10-01" @default.
- W1528649928 modified "2023-10-03" @default.
- W1528649928 title "Amino Acid Levels and Enzyme Activity in Tissues of Rats Force-fed Diets Differing in Methionine Content" @default.
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- W1528649928 doi "https://doi.org/10.1093/jn/99.2.145" @default.
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