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- W1528658466 abstract "To the Editor:We want to thank Knaak et al. (1) for their interest in ourresearchworkandfortheircomplimentsonourstudy(2).Inresponse to their minor comments we would like to makethe following remarks:(1) We disagree that the rise in ALT within the first hour ofperfusion can be interpreted as ongoing ischemiaduring that time period. We used a closed perfusionsystem and concentrations of ALT are a result ofcumulative washout of transaminases from the graftafter cold preservation, as has been shown in severalanimal studies (3). Values obtained in our ex vivosystem cannot be interpreted the same way as in vivovalues in blood of patients, as the perfusion circuitcontains only 2 L compared to an average of 5 L ofblood in vivo. Moreover, we strongly disagree thatthere was ongoing ischemic damage due to hypoper-fusion during the first hour. First, as shown in Figure 4there were no histological signs of zone III injury after6 h of perfusion. Second, it is a mistake to extrapolateflow data obtained from in vivo studies in cirrhoticpatientswith portal hypertensionsuchas by Kelly etal.one-to-one to the ex vivo settings used in our system.O" @default.
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- W1528658466 date "2013-09-01" @default.
- W1528658466 modified "2023-10-16" @default.
- W1528658466 title "Normothermic Machine Perfusion of Discarded Liver Grafts" @default.
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- W1528658466 doi "https://doi.org/10.1111/ajt.12374" @default.
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