Matches in SemOpenAlex for { <https://semopenalex.org/work/W1528698913> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1528698913 endingPage "42" @default.
- W1528698913 startingPage "34" @default.
- W1528698913 abstract "The accurate diagnosis of tuberculosis caused by members of the Mycobacterium tuberculosis complex (MTBC) has remained a major challenge in clinical laboratories world-wide. Several studies have evaluated the use of highly specific in-house PCR assays targeting the IS6110, hupB, rpoB, oxyR, and IS1081 genes in the detection of MTBC species with reports on variable sensitivities depending on the geographical sourcing of isolates. In the present investigations, we evaluated the sensitivities of these PCR assays on 125 MTBC cultured isolates from five (West, Centre, Littoral, North West and South West) of the ten regions of Cameroon. Of this number, 124 (99.2%), 117 (93.6%), 123 (99.1%), 119 (95.2%) and 118 (94.4%) were positive by the IS6110, hupB, rpoB, oxyR, and IS1081-based PCR assays respectively. A total of 110 (88%) of the cultured isolates were also identified as MTBC by standard biochemical tests. Of this number, 109 (99.1%), 104 (94.5%), 109 (99.1%), 106 (96.4%) and 104 (94.5%) were positive in the IS6110, hupB, rpoB, oxyR, and IS1081-based PCR assays respectively. Concordant PCR results were obtained for 108 of the 125 samples. The 15 isolates that were negative biochemically scored sensitivities ranging from 100% (for the IS6110 assay) to 86.7% (for the hupB and oxyR assay). The combination of the IS6110 assay, which turned out to be the most sensitive, and each of the other assays gave 100% sensitivity. We conclude that the combined targeting of the IS6110 and rpoB genes is likely to yield the most sensitive PCR procedure for the diagnosis of MTBC infection in the five regions of Cameroon." @default.
- W1528698913 created "2016-06-24" @default.
- W1528698913 creator A5003618551 @default.
- W1528698913 creator A5004234277 @default.
- W1528698913 creator A5020483534 @default.
- W1528698913 creator A5023586737 @default.
- W1528698913 creator A5033010156 @default.
- W1528698913 creator A5052529834 @default.
- W1528698913 date "2015-01-01" @default.
- W1528698913 modified "2023-10-17" @default.
- W1528698913 title "Comparison of the Sensitivities of Different PCR Assays for the Detection of Mycobacterium tuberculosis Complex Isolates from Five Regions of Cameroon" @default.
- W1528698913 cites W1531374249 @default.
- W1528698913 cites W1884882993 @default.
- W1528698913 cites W1968649252 @default.
- W1528698913 cites W1988908300 @default.
- W1528698913 cites W2015069060 @default.
- W1528698913 cites W2062358224 @default.
- W1528698913 cites W2089427964 @default.
- W1528698913 cites W2113991352 @default.
- W1528698913 cites W2114658413 @default.
- W1528698913 cites W2120254719 @default.
- W1528698913 cites W2120418557 @default.
- W1528698913 cites W2145392766 @default.
- W1528698913 cites W2163047037 @default.
- W1528698913 cites W2168627120 @default.
- W1528698913 cites W2170457111 @default.
- W1528698913 cites W300344787 @default.
- W1528698913 doi "https://doi.org/10.4236/jtr.2015.32005" @default.
- W1528698913 hasPublicationYear "2015" @default.
- W1528698913 type Work @default.
- W1528698913 sameAs 1528698913 @default.
- W1528698913 citedByCount "2" @default.
- W1528698913 countsByYear W15286989132018 @default.
- W1528698913 countsByYear W15286989132020 @default.
- W1528698913 crossrefType "journal-article" @default.
- W1528698913 hasAuthorship W1528698913A5003618551 @default.
- W1528698913 hasAuthorship W1528698913A5004234277 @default.
- W1528698913 hasAuthorship W1528698913A5020483534 @default.
- W1528698913 hasAuthorship W1528698913A5023586737 @default.
- W1528698913 hasAuthorship W1528698913A5033010156 @default.
- W1528698913 hasAuthorship W1528698913A5052529834 @default.
- W1528698913 hasBestOaLocation W15286989131 @default.
- W1528698913 hasConcept C104317684 @default.
- W1528698913 hasConcept C142724271 @default.
- W1528698913 hasConcept C159047783 @default.
- W1528698913 hasConcept C2775891814 @default.
- W1528698913 hasConcept C2777975735 @default.
- W1528698913 hasConcept C2778340795 @default.
- W1528698913 hasConcept C2779790121 @default.
- W1528698913 hasConcept C2780311561 @default.
- W1528698913 hasConcept C2781069245 @default.
- W1528698913 hasConcept C49105822 @default.
- W1528698913 hasConcept C54355233 @default.
- W1528698913 hasConcept C6557445 @default.
- W1528698913 hasConcept C71924100 @default.
- W1528698913 hasConcept C86803240 @default.
- W1528698913 hasConcept C89423630 @default.
- W1528698913 hasConceptScore W1528698913C104317684 @default.
- W1528698913 hasConceptScore W1528698913C142724271 @default.
- W1528698913 hasConceptScore W1528698913C159047783 @default.
- W1528698913 hasConceptScore W1528698913C2775891814 @default.
- W1528698913 hasConceptScore W1528698913C2777975735 @default.
- W1528698913 hasConceptScore W1528698913C2778340795 @default.
- W1528698913 hasConceptScore W1528698913C2779790121 @default.
- W1528698913 hasConceptScore W1528698913C2780311561 @default.
- W1528698913 hasConceptScore W1528698913C2781069245 @default.
- W1528698913 hasConceptScore W1528698913C49105822 @default.
- W1528698913 hasConceptScore W1528698913C54355233 @default.
- W1528698913 hasConceptScore W1528698913C6557445 @default.
- W1528698913 hasConceptScore W1528698913C71924100 @default.
- W1528698913 hasConceptScore W1528698913C86803240 @default.
- W1528698913 hasConceptScore W1528698913C89423630 @default.
- W1528698913 hasIssue "02" @default.
- W1528698913 hasLocation W15286989131 @default.
- W1528698913 hasOpenAccess W1528698913 @default.
- W1528698913 hasPrimaryLocation W15286989131 @default.
- W1528698913 hasRelatedWork W1567338735 @default.
- W1528698913 hasRelatedWork W1903979725 @default.
- W1528698913 hasRelatedWork W2006509003 @default.
- W1528698913 hasRelatedWork W2028105514 @default.
- W1528698913 hasRelatedWork W2127158526 @default.
- W1528698913 hasRelatedWork W2146456540 @default.
- W1528698913 hasRelatedWork W2399991959 @default.
- W1528698913 hasRelatedWork W2938138842 @default.
- W1528698913 hasRelatedWork W3116175965 @default.
- W1528698913 hasRelatedWork W4307178141 @default.
- W1528698913 hasVolume "03" @default.
- W1528698913 isParatext "false" @default.
- W1528698913 isRetracted "false" @default.
- W1528698913 magId "1528698913" @default.
- W1528698913 workType "article" @default.