FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1528752018> ?p ?o ?g. }

• W1528752018 endingPage "543" @default.
• W1528752018 startingPage "537" @default.
• W1528752018 abstract "We demonstrate, for the first time in fish, that a Ca2+-independent and cyclic-nucleotide-independent histone H1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them. The kinase, M-phase-specific histone H1 kinase (M-H1K), was purified from mature carp oocytes by using two exogenous substrates for assaying its activity: histone H1 and a synthetic peptide (SP peptide, KKAAKSPKKAKK) containing the sequence KSPKK, which includes the consensus sequence of the site phosphorylated by a serine/threonine-specific protein kinase encoded by the fission yeast cdc2+ gene (cdc2 kinase). The M-H1K and maturation-promoting factor (MPF) activities coincided closely throughout four steps of purification, strongly suggesting the identity of M-H1K and MPF. The final preparation was purified 5000-fold with a recovery of 4%, when histone H1 was used for the kinase assay, and 10000-fold with a recovery of 7% when SP peptide was used. The purified molecular mass of the kinase was estimated to be 100 kDa by gel filtration and contained four proteins of 33,34,46 and 48 kDa. Anti-PSTAIR antibody recognizing cdc2 kinase cross-reacted with the 33-kDa and 34-kDa proteins, while the 46-kDa and 48-kDa bands cross-reacted with monoclonal antibodies raised against cyclin B. The 33-kDa protein was also recognized by an antibody against a goldfish cdk2 (Eg1) kinase, a cdc2-related kinase which has the PSTAIR sequence and binds to p13suc1 but does not form a complex with cyclin B. M-H1K activity corresponded well to the 34-kDa, 46-kDa and 48-kDa proteins but not to the 33-kDa protein. These results strongly suggest that M-H1K consists of cdc2 kinase forming a complex with cyclin B, and that cdk2 kinase is not a component of M-H1K, although it is found in the highly purified M-H1K. The purified M-H1K utilized Mg2+, Mn2+, ATP and GTP, and had a wide pH optimum ranging over 8.0–10.5. The kinase was thermolabile and sensitive to freezing/thawing." @default.
• W1528752018 created "2016-06-24" @default.
• W1528752018 creator A5003931302 @default.
• W1528752018 creator A5020600038 @default.
• W1528752018 creator A5021937825 @default.
• W1528752018 creator A5032367423 @default.
• W1528752018 creator A5057717292 @default.
• W1528752018 creator A5061728715 @default.
• W1528752018 creator A5069144309 @default.
• W1528752018 creator A5070064607 @default.
• W1528752018 creator A5078854680 @default.
• W1528752018 date "1992-04-01" @default.
• W1528752018 modified "2023-10-18" @default.
• W1528752018 title "M-phase-specific histone H1 kinase in fish oocytes. Purification, components and biochemical properties" @default.
• W1528752018 cites W1001167770 @default.
• W1528752018 cites W132788771 @default.
• W1528752018 cites W1512390959 @default.
• W1528752018 cites W1547579138 @default.
• W1528752018 cites W1558051976 @default.
• W1528752018 cites W1566217027 @default.
• W1528752018 cites W1583109919 @default.
• W1528752018 cites W1587213566 @default.
• W1528752018 cites W1595865541 @default.
• W1528752018 cites W1723327061 @default.
• W1528752018 cites W1802783087 @default.
• W1528752018 cites W1873949485 @default.
• W1528752018 cites W1898229949 @default.
• W1528752018 cites W1973461983 @default.
• W1528752018 cites W1977646528 @default.
• W1528752018 cites W1979629909 @default.
• W1528752018 cites W1985232413 @default.
• W1528752018 cites W1992697092 @default.
• W1528752018 cites W2000146554 @default.
• W1528752018 cites W2008066024 @default.
• W1528752018 cites W2014234155 @default.
• W1528752018 cites W2014445311 @default.
• W1528752018 cites W2014484676 @default.
• W1528752018 cites W2015150670 @default.
• W1528752018 cites W2016043069 @default.
• W1528752018 cites W2031576542 @default.
• W1528752018 cites W2032413267 @default.
• W1528752018 cites W2037149474 @default.
• W1528752018 cites W2037355625 @default.
• W1528752018 cites W2059577145 @default.
• W1528752018 cites W2064755731 @default.
• W1528752018 cites W2066899718 @default.
• W1528752018 cites W2067109731 @default.
• W1528752018 cites W2071732331 @default.
• W1528752018 cites W2077262533 @default.
• W1528752018 cites W2081290676 @default.
• W1528752018 cites W2090827556 @default.
• W1528752018 cites W2092455067 @default.
• W1528752018 cites W2100238612 @default.
• W1528752018 cites W2100837269 @default.
• W1528752018 cites W2102242413 @default.
• W1528752018 cites W2107423274 @default.
• W1528752018 cites W2114685781 @default.
• W1528752018 cites W2120626070 @default.
• W1528752018 cites W2123930515 @default.
• W1528752018 cites W2127283099 @default.
• W1528752018 cites W2138430171 @default.
• W1528752018 doi "https://doi.org/10.1111/j.1432-1033.1992.tb16810.x" @default.
• W1528752018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1315270" @default.
• W1528752018 hasPublicationYear "1992" @default.
• W1528752018 type Work @default.
• W1528752018 sameAs 1528752018 @default.
• W1528752018 citedByCount "53" @default.
• W1528752018 countsByYear W15287520182012 @default.
• W1528752018 countsByYear W15287520182013 @default.
• W1528752018 countsByYear W15287520182014 @default.
• W1528752018 countsByYear W15287520182016 @default.
• W1528752018 countsByYear W15287520182023 @default.
• W1528752018 crossrefType "journal-article" @default.
• W1528752018 hasAuthorship W1528752018A5003931302 @default.
• W1528752018 hasAuthorship W1528752018A5020600038 @default.
• W1528752018 hasAuthorship W1528752018A5021937825 @default.
• W1528752018 hasAuthorship W1528752018A5032367423 @default.
• W1528752018 hasAuthorship W1528752018A5057717292 @default.
• W1528752018 hasAuthorship W1528752018A5061728715 @default.
• W1528752018 hasAuthorship W1528752018A5069144309 @default.
• W1528752018 hasAuthorship W1528752018A5070064607 @default.
• W1528752018 hasAuthorship W1528752018A5078854680 @default.
• W1528752018 hasConcept C101152365 @default.
• W1528752018 hasConcept C104317684 @default.
• W1528752018 hasConcept C120504264 @default.
• W1528752018 hasConcept C137361374 @default.
• W1528752018 hasConcept C153911025 @default.
• W1528752018 hasConcept C159479382 @default.
• W1528752018 hasConcept C184235292 @default.
• W1528752018 hasConcept C29537977 @default.
• W1528752018 hasConcept C51853150 @default.
• W1528752018 hasConcept C55493867 @default.
• W1528752018 hasConcept C59143045 @default.
• W1528752018 hasConcept C64927066 @default.
• W1528752018 hasConcept C6902291 @default.
• W1528752018 hasConcept C81963 @default.
• W1528752018 hasConcept C82495950 @default.
• W1528752018 hasConcept C86803240 @default.

• next